Yeast KEX1 gene encodes a putative protease with a carboxypeptidase B-like function involved in killer toxin and α-factor precursor processing

DNA sequencing with chain-terminating inhibitors

1977 • 69,181 citations

Detection of specific sequences among DNA fragments separated by gel electrophoresis

1975 • 33,059 citations

A simple method for displaying the hydropathic character of a protein

1982 • 22,471 citations

Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectors

1985 • 15,222 citations

Studies on transformation of Escherichia coli with plasmids

1983 • 11,179 citations

Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.

1980 • 8,711 citations

Methods in yeast genetics

1979 • 7,824 citations

Transformation of intact yeast cells treated with alkali cations

1983 • 7,688 citations

[12] One-step gene disruption in yeast

1983 • 3,302 citations

Transformation of yeast.

1978 • 2,267 citations

DNA sequence required for efficient transcription termination in yeast

1982 • 1,014 citations

The sequence 5′-AAUAAA-3′ forms part of the recognition site for polyadenylation of late SV40 mRNAs

1981 • 819 citations

An electrophoretic karyotype for yeast.

1985 • 787 citations

Compilation of published signal sequences

1984 • 784 citations

Oligonucleotide-directed mutagenesis using M13-derived vectors: an efficient and general procedure for the production of point mutations in any fragment of DNA

1982 • 764 citations

How signal sequences maintain cleavage specificity

1984 • 725 citations

Isolation of the putative structural gene for the lysine-arginine-cleaving endopeptidase required for processing of yeast prepro-α-factor

1984 • 698 citations

Structure of a yeast pheromone gene (MFα): A putative α-factor precursor contains four tandem copies of mature α-factor

1982 • 587 citations

[49] Rapid DNA isolations for enzymatic and hybridization analysis

1980 • 572 citations

POLYPROTEIN GENE EXPRESSION: Generation of Diversity of Neuroendocrine Peptides

1984 • 497 citations

PROCESSING MECHANISMS IN THE BIOSYNTHESIS OF PROTEINS*

1980 • 461 citations

Glycosylation and processing of prepro-α-factor through the yeast secretory pathway

1984 • 410 citations

Yeast α factor is processed from a larger precursor polypeptide: The essential role of a membrane-bound dipeptidyl aminopeptidase

1983 • 403 citations

PEP4 gene of Saccharomyces cerevisiae encodes proteinase A, a vacuolar enzyme required for processing of vacuolar precursors.

1986 • 400 citations

Evidence for an intron-contained sequence required for the splicing of yeast RNA polymerase II transcripts

1983 • 396 citations

Protein sorting in yeast: The localization determinant of yeast vacuolar carboxypeptidase Y resides in the propeptide

1987 • 329 citations

The PEP4 Gene Encodes an Aspartyl Protease Implicated in the Posttranslational Regulation of Saccharomyces cerevisiae Vacuolar Hydrolases

1986 • 292 citations

Purification and characterization of enkephalin convertase, an enkephalin-synthesizing carboxypeptidase.

1983 • 265 citations

The processing of peptide precursors

1986 • 249 citations

Secretion and processing of insulin precursors in yeast.

1986 • 227 citations

Physiological characterization of Saccharomyces cerevisiae mutants supersensitive to G1 arrest by a factor and alpha factor pheromones.

1982 • 216 citations

Sequence of the preprotoxin dsRNA gene of type I killer yeast: Multiple processing events produce a two-component toxin

1984 • 214 citations

Cloning and sequence analysis of cDNA for bovine carboxypeptidase E

1986 • 196 citations

Cloning of Saccharomyces cerevisiae DNA replication genes: isolation of the CDC8 gene and two genes that compensate for the cdc8-1 mutation.

1983 • 192 citations

The PEP4 gene encodes an aspartyl protease implicated in the posttranslational regulation of Saccharomyces cerevisiae vacuolar hydrolases.

1986 • 192 citations

Serine carboxypeptidases. A review

1986 • 183 citations

NEUROPEPTIDES: MULTIPLE MOLECULAR FORMS, METABOLIC PATHWAYS, AND RECEPTORS

1986 • 177 citations

Saccharomyces cerevisiaecontains two discrete genes coding for the α-factor pheromone

1983 • 165 citations

RAD6 gene of Saccharomyces cerevisiae encodes a protein containing a tract of 13 consecutive aspartates.

1985 • 151 citations

Control of yeast cell type by the mating type locus: Positive regulation of the α-specific STE3 gene by the MATα 1 product

1983 • 135 citations

TWO CHROMOSOMAL GENES REQUIRED FOR KILLING EXPRESSION IN KILLER STRAINS OF SACCHAROMYCES CEREVISIAE

1976 • 127 citations

The Kinetics of Carboxypeptidase B Activity

1962 • 118 citations

Hormone processing and membrane-bound proteinases in yeast.

1985 • 114 citations

Preparation and characterization of highly radioactive in vitro labeled adenovirus DNA and DNA restriction fragments

1977 • 106 citations

A chromosomal gene required for killer plasmid expression, mating, and spore maturation in Saccharomyces cerevisiae.

1976 • 99 citations

Alpha-factor structural gene mutations in Saccharomyces cerevisiae: effects on alpha-factor production and mating.

1985 • 93 citations

Yeast killer toxin: Site-directed mutations implicate the precursor protein as the immunity component

1986 • 87 citations

Secretion ofSaccharomyces cerevisiaeKiller Toxin: Processing of the Glycosylated Precursor

1983 • 85 citations

Cloning and sequencing of the preprotoxin-coding region of the yeast M1 double-stranded RNA.

1984 • 80 citations

Yeast killer plasmid mutations affecting toxin secretion and activity and toxin immunity function.

1982 • 73 citations

Evidence for an essential histidine in carboxypeptidase Y. Reaction with the chloromethyl ketone derivative of benzyloxycarbonyl-L-phenylalanine.

1975 • 67 citations

The expression of cDNA clones of yeast M1 double-stranded RNA in yeast confers both killer and immunity phenotypes.

1984 • 57 citations

A Functional Prepro-α-Factor Gene in Saccharomyces Yeasts Can Contain Three, Four, or Five Repeats of the Mature Pheromone Sequence

1983 • 54 citations

Solid phase peptide synthesis of α-factor, a yeast mating pheromone

1977 • 53 citations

Sequence and expression of the dCMP deaminase gene (DCD1) of Saccharomyces cerevisiae.

1986 • 49 citations

Determination of the carboxyl termini of the alpha and beta subunits of yeast K1 killer toxin. Requirement of a carboxypeptidase B-like activity for maturation.

1987 • 44 citations

From organelle to organelle

1987 • 37 citations

Structure and expression of the M₂genomic segment of a type 2 killer virus of yeast

1985 • 36 citations

Amino acid sequence of carboxypeptidase Y. II. Peptides from enzymatic cleavages

1982 • 31 citations

In Vivo Evidence for Posttranslational Translocation and Signal Cleavage of the Killer Preprotoxin of Saccharomyces cerevisiae

1986 • 31 citations

A functional prepro-alpha-factor gene in Saccharomyces yeasts can contain three, four, or five repeats of the mature pheromone sequence.

1983 • 30 citations

In vivo evidence for posttranslational translocation and signal cleavage of the killer preprotoxin of Saccharomyces cerevisiae.

1986 • 22 citations

Radiochemical determination of a unique sequence around the reactive serine residue of a di-isopropyl phosphorofluoridate-sensitive plant carboxypeptidase and a yeast peptidase

1972 • 20 citations

Amino acid sequence of carboxypeptidase Y. I. Peptides from cleavage with cyanogen bromide

1982 • 18 citations