nup1 mutants exhibit pleiotropic defects in nuclear pore complex function.

A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae.

1989 • 8,705 citations

Methods in yeast genetics

1979 • 7,824 citations

Transformation of intact yeast cells treated with alkali cations

1983 • 7,688 citations

Rapid and efficient site-specific mutagenesis without phenotypic selection.

1985 • 7,573 citations

[19] Rapid and efficient site-specific mutagenesis without phenotypic selection

1987 • 5,413 citations

Unidirectional digestion with exonuclease III creates targeted breakpoints for DNA sequencing

1984 • 4,231 citations

A positive selection for mutants lacking orotidine-5′-phosphate decarboxylase activity in yeast: 5-fluoro-orotic acid resistance

1984 • 2,401 citations

From cadherins to catenins: cytoplasmic protein interactions and regulation of cell adhesion

1993 • 997 citations

Structural rearrangements of tubulin and actin during the cell cycle of the yeast Saccharomyces.

1984 • 827 citations

Identification and characterization of a nuclear pore complex protein

1986 • 610 citations

How proteins enter the nucleus

1991 • 555 citations

Monoclonal antibodies identify a group of nuclear pore complex glycoproteins.

1987 • 533 citations

Inhibition of in vitro nuclear transport by a lectin that binds to nuclear pores.

1987 • 510 citations

Correlation between structure and mass distribution of the nuclear pore complex and of distinct pore complex components.

1990 • 510 citations

Architecture and design of the nuclear pore complex

1992 • 452 citations

Nuclear pore complex glycoproteins contain cytoplasmically disposed O-linked N-acetylglucosamine.

1987 • 428 citations

Functions of microtubules in the Saccharomyces cerevisiae cell cycle.

1988 • 410 citations

Architecture of the Xenopus nuclear pore complex revealed by three-dimensional cryo-electron microscopy

1993 • 408 citations

Cytosolic proteins that specifically bind nuclear location signals are receptors for nuclear import

1991 • 391 citations

THE NUCLEAR PORE COMPLEX

1995 • 383 citations

Isolation and characterization of RAT1: an essential gene of Saccharomyces cerevisiae required for the efficient nucleocytoplasmic trafficking of mRNA.

1992 • 370 citations

Nuclear pore complex contains a family of glycoproteins that includes p62: glycosylation through a previously unidentified cellular pathway.

1987 • 330 citations

A large particle associated with the perimeter of the nuclear pore complex.

1982 • 314 citations

A nuclear pore complex protein that contains zinc finger motifs, binds DNA, and faces the nucleoplasm

1993 • 308 citations

AGENTS THAT CAUSE A HIGH FREQUENCY OF GENETIC CHANGE FROM [psi +] TO [psi -] IN SACCHAROMYCES CEREVISIAE

1981 • 303 citations

O-linked N-acetylglucosamine is attached to proteins of the nuclear pore. Evidence for cytoplasmic and nucleoplasmic glycoproteins.

1987 • 268 citations

A new family of yeast nuclear pore complex proteins.

1992 • 256 citations

Toward a more complete 3-D structure of the nuclear pore complex

1991 • 254 citations

Structure and Function of the Nuclear Pore Complex

1992 • 237 citations

Reversible inhibition of protein import into the nucleus by wheat germ agglutinin injected into cultured cells

1987 • 233 citations

The yeast RNA1 gene product necessary for RNA processing is located in the cytosol and apparently excluded from the nucleus.

1990 • 228 citations

Reconstitution of biochemically altered nuclear pores: Transport can be eliminated and restored

1990 • 228 citations

High resolution scanning electron microscopy of the nuclear envelope: demonstration of a new, regular, fibrous lattice attached to the baskets of the nucleoplasmic face of the nuclear pores.

1992 • 225 citations

An integral membrane protein of the pore membrane domain of the nuclear envelope contains a nucleoporin-like region

1993 • 220 citations

The NUP1 gene encodes an essential component of the yeast nuclear pore complex

1990 • 219 citations

A temperature-sensitive NUP116 null mutant forms a nuclear envelope seal over the yeast nuclear pore complex thereby blocking nucleocytoplasmic traffic.

1993 • 211 citations

A monoclonal antibody against the nuclear pore complex inhibits nucleocytoplasmic transport of protein and RNA in vivo.

1988 • 206 citations

Amino acid sequences that determine the nuclear localization of yeast histone 2B.

1987 • 194 citations

Purification of NSP1 reveals complex formation with ‘GLFG’ nucleoporins and a novel nuclear pore protein NIC96.

1993 • 189 citations

NSP1: A yeast nuclear envelope protein localized at the nuclear pores exerts its essential function by its carboxy-terminal domain

1990 • 180 citations

BIK1, a protein required for microtubule function during mating and mitosis in Saccharomyces cerevisiae, colocalizes with tubulin.

1990 • 178 citations

The NSR1 gene encodes a protein that specifically binds nuclear localization sequences and has two RNA recognition motifs.

1991 • 173 citations

A new subclass of nucleoporins that functionally interact with nuclear pore protein NSP1.

1992 • 172 citations

Astral microtubules are not required for anaphase B in Saccharomyces cerevisiae.

1992 • 171 citations

The nuclear pore complex

1993 • 166 citations

Nuclear PRP20 protein is required for mRNA export.

1993 • 148 citations

Inhibition of nuclear accumulation of karyophilic proteins in living cells by microinjection of the lectin wheat germ agglutinin

1988 • 146 citations

Defects in mRNA 3'-end formation, transcription initiation, and mRNA transport associated with the yeast mutation prp20: possible coupling of mRNA processing and chromatin structure.

1992 • 142 citations

Primary sequence and heterologous expression of nuclear pore glycoprotein p62.

1990 • 137 citations

Yeast Srp1p has homology to armadillo/plakoglobin/beta-catenin and participates in apparently multiple nuclear functions including the maintenance of the nucleolar structure.

1994 • 128 citations

Genetic and physical interactions between Srp1p and nuclear pore complex proteins Nup1p and Nup2p.

1994 • 125 citations

Nuclear pore complex glycoprotein p62 of Xenopus laevis and mouse: cDNA cloning and identification of its glycosylated region.

1991 • 123 citations

NUP2, a novel yeast nucleoporin, has functional overlap with other proteins of the nuclear pore complex.

1993 • 119 citations

Yeast nuclear envelope proteins cross react with an antibody against mammalian pore complex proteins.

1989 • 112 citations

A conditional yeast mutant deficient in mRNA transport from nucleus to cytoplasm.

1992 • 112 citations

Human nucleoporin p62 and the essential yeast nuclear pore protein NSP1 show sequence homology and a similar domain organization.

1991 • 104 citations

[41] Preparation of yeast cells for thin-section electron microscopy

1991 • 97 citations

A functional homologue of the RNA1 gene product in Schizosaccharomyces pombe: purification, biochemical characterization, and identification of a leucine-rich repeat motif.

1993 • 93 citations

The Role of Cytoplasmic Membranes in Controlling the Transport of Nuclear Messenger RNA and Initiation of Protein Synthesis

1974 • 86 citations

Amino Acid Sequences That Determine the Nuclear Localization of Yeast Histone 2B

1987 • 86 citations

O-linked glycoproteins of the nuclear pore complex interact with a cytosolic factor required for nuclear protein import.

1992 • 66 citations

Structural and functional analyses of Saccharomyces cerevisiae wild-type and mutant RNA1 genes.

1989 • 65 citations

In vitro translocation through the yeast nuclear envelope

1989 • 63 citations

NSP1 depletion in yeast affects nuclear pore formation and nuclear accumulation.

1992 • 53 citations

Targeting of a cytosolic protein to the nuclear periphery.

1990 • 32 citations

Structural and Functional Analyses of Saccharomyces cerevisiae Wild-Type and Mutant RNA1 Genes

1989 • 29 citations

Control of nucleocytoplasmic transport

1992 • 26 citations

Cell-type control of membrane biogenesis induced by HMG-CoA reductase.

1990 • 21 citations