Small heat shock proteins: molecular structure and chaperone function

Protein folding and misfolding

2003 • 4,484 citations

Hsp70 chaperones: Cellular functions and molecular mechanism

2005 • 2,649 citations

Macromolecular crowding: obvious but underappreciated

2001 • 2,198 citations

RNA-Binding Proteins Tia-1 and Tiar Link the Phosphorylation of Eif-2α to the Assembly of Mammalian Stress Granules

1999 • 1,169 citations

Folding of Newly Translated Proteins In Vivo: The Role of Molecular Chaperones

2001 • 1,157 citations

Pathways of chaperone-mediated protein folding in the cytosol

2004 • 1,092 citations

A missense mutation in the αB-crystallin chaperone gene causes a desmin-related myopathy

1998 • 1,061 citations

Crystal structure of a small heat-shock protein

1998 • 876 citations

Join the crowd

2003 • 846 citations

A small heat shock protein stably binds heat-denatured model substrates and can maintain a substrate in a folding-competent state

1997 • 772 citations

Binding of non-native protein to Hsp25 during heat shock creates a reservoir of folding intermediates for reactivation

1997 • 750 citations

Regulation of Hsp27 Oligomerization, Chaperone Function, and Protective Activity against Oxidative Stress/Tumor Necrosis Factor α by Phosphorylation

1999 • 716 citations

Regulation of actin filament dynamics by p38 map kinase-mediated phosphorylation of heat shock protein 27

1997 • 704 citations

Crystal structure and assembly of a eukaryotic small heat shock protein.

2001 • 698 citations

CHIP and Hsp70 regulate tau ubiquitination, degradation and aggregation

2004 • 673 citations

Small heat shock proteins and stress tolerance in plants

2002 • 628 citations

α-Crystallin-Type Heat Shock Proteins: Socializing Minichaperones in the Context of a Multichaperone Network

2002 • 528 citations

Phosphorylation and supramolecular organization of murine small heat shock protein HSP25 abolish its actin polymerization-inhibiting activity.

1994 • 493 citations

HSP27 phosphorylation-mediated resistance against actin fragmentation and cell death induced by oxidative stress.

1996 • 471 citations

Hsp26: a temperature-regulated chaperone

1999 • 446 citations

Autosomal dominant congenital cataract associated with a missense mutation in the human alpha crystallin gene CRYAA

1998 • 441 citations

Mutation R120G in αB-crystallin, which is linked to a desmin-related myopathy, results in an irregular structure and defective chaperone-like function

1999 • 369 citations

Intermediate filament interactions can be altered by HSP27 and αB-crystallin

1999 • 359 citations

Actin cytoskeleton and small heat shock proteins: how do they interact?

2002 • 352 citations

Small heat-shock proteins regulate membrane lipid polymorphism

2002 • 326 citations

HSP27 Multimerization Mediated by Phosphorylation-sensitive Intermolecular Interactions at the Amino Terminus

1999 • 324 citations

The small heat shock proteins and their role in human disease

2005 • 315 citations

Membrane physical state controls the signaling mechanism of the heat shock response in Synechocystis PCC 6803: Identification of hsp17 as a “fluidity gene”

1998 • 310 citations

sHsps and their role in the chaperone network

2002 • 288 citations

Subunit Exchange of αA-Crystallin

1997 • 286 citations

Synechocystis HSP17 is an amphitropic protein that stabilizes heat-stressed membranes and binds denatured proteins for subsequent chaperone-mediated refolding

2001 • 264 citations

Structure and function of small heat shock/α-crystallin proteins: established concepts and emerging ideas

2000 • 254 citations

The Tomato Hsf System: HsfA2 Needs Interaction with HsfA1 for Efficient Nuclear Import and May Be Localized in Cytoplasmic Heat Stress Granules

1998 • 251 citations

Subunit Exchange of Small Heat Shock Proteins

2000 • 239 citations

Mycobacterium tuberculosis 16-kDa Antigen (Hsp16.3) Functions as an Oligomeric Structure in Vitro to Suppress Thermal Aggregation

1996 • 230 citations

Structure-Function Studies on Small Heat Shock Protein Oligomeric Assembly and Interaction with Unfolded Polypeptides

1997 • 223 citations

The Small Heat Shock Protein αB-crystallin Is a Novel Inhibitor of TRAIL-induced Apoptosis That Suppresses the Activation of Caspase-3

2005 • 217 citations

Life in a crowded world

2004 • 208 citations

Hsp42 is the general small heat shock protein in the cytosol of Saccharomyces cerevisiae

2004 • 205 citations

Subunit Exchange of Multimeric Protein Complexes

2002 • 194 citations

Small heat-shock protein structures reveal a continuum from symmetric to variable assemblies

2000 • 193 citations

The Cardiomyopathy and Lens Cataract Mutation in αB-crystallin Alters Its Protein Structure, Chaperone Activity, and Interaction with Intermediate Filaments in Vitro

1999 • 186 citations

Analysis of the Interaction of Small Heat Shock Proteins with Unfolding Proteins

2003 • 180 citations

Evolutionary Diversity of Vertebrate Small Heat Shock Proteins

2004 • 178 citations

Temperature-dependent Chaperone Activity and Structural Properties of Human αA- and αB-crystallins

2000 • 170 citations

The Dynamics of Hsp25 Quaternary Structure

1999 • 166 citations

Thermal Dissociation of Multimeric Protein Complexes by Using Nanoelectrospray Mass Spectrometry

2003 • 165 citations

Cloning, Expression, and Chaperone-like Activity of Human αA-Crystallin

1996 • 163 citations

The Identity of Proteins Associated with a Small Heat Shock Protein during Heat Stress in Vivo Indicates That These Chaperones Protect a Wide Range of Cellular Functions

2004 • 162 citations

Small heat shock protein of Methanococcus jannaschii , a hyperthermophile

1998 • 157 citations

The Synthesis of a Small Heat Shock/α-Crystallin Protein inArtemiaand Its Relationship to Stress Tolerance during Development

1999 • 155 citations

Effects of Site-directed Mutations on the Chaperone-like Activity of αB-Crystallin

1996 • 146 citations

α‐crystallin: a review of its structure and function

2004 • 146 citations

Analysis of the Regulation of the Molecular Chaperone Hsp26 by Temperature-induced Dissociation

2004 • 134 citations

Subunit Exchange, Conformational Stability, and Chaperone-like Function of the Small Heat Shock Protein 16.5 fromMethanococcus jannaschii

2002 • 127 citations

Immobilization of the C-terminal Extension of Bovine αA-Crystallin Reduces Chaperone-like Activity

1996 • 126 citations

Mouse Hsp25, a small heat shock protein

2000 • 125 citations

αB-Crystallin Modulates Protein Aggregation of Abnormal Desmin

2003 • 124 citations

Characterization of α-Crystallin-Plasma Membrane Binding

2000 • 123 citations

Some properties of human small heat shock protein Hsp20 (HspB6)

2003 • 120 citations

The Interaction of the Molecular Chaperone α-Crystallin with Unfolding α-Lactalbumin: A Structural and Kinetic Spectroscopic Study

2002 • 119 citations

Mammalian Hsp22 is a heat-inducible small heat-shock protein with chaperone-like activity

2004 • 119 citations

The C-terminal region of α-crystallin: involvement in protection against heat-induced denaturation

1993 • 118 citations

Expression and aggregation of recombinant αA-crystallin and its two domains

1992 • 116 citations

Essential Role of the NH2-terminal WD/EPF Motif in the Phosphorylation-activated Protective Function of Mammalian Hsp27

2004 • 115 citations

Molecular chaperones, stress resistance and development in Artemia franciscana

2003 • 115 citations

Cytotoxic effects induced by oxidative stress in cultured mammalian cells and protection provided by Hsp27 expression

2004 • 115 citations

Role of ATP on the Interaction of α-Crystallin with Its Substrates and Its Implications for the Molecular Chaperone Function

2004 • 113 citations

Subunit Exchange of Polydisperse Proteins

2005 • 113 citations

Chaperone activity of cytosolic small heat shock proteins from wheat

2004 • 113 citations

A Novel Quaternary Structure of the Dimeric α-Crystallin Domain with Chaperone-like Activity

2001 • 112 citations

Mechanism of Chaperone Function in Small Heat-shock Proteins

2003 • 112 citations

Folding pattern of the α-crystallin domain in αA-crystallin determined by site-directed spin labeling

1999 • 112 citations

Differential Temperature-dependent Chaperone-like Activity of αA- and αB-crystallin Homoaggregates

1999 • 111 citations

The IXI/V motif in the C-terminal extension of alpha-crystallins: alternative interactions and oligomeric assemblies.

2004 • 110 citations

Interactions between Small Heat Shock Protein Subunits and Substrate in Small Heat Shock Protein-Substrate Complexes

2004 • 110 citations

The Small Heat Shock Protein IbpA of Escherichia coli Cooperates with IbpB in Stabilization of Thermally Aggregated Proteins in a Disaggregation Competent State

2005 • 108 citations

Intermolecular Exchange and Stabilization of Recombinant Human αA- and αB-Crystallin

1998 • 103 citations

On the interaction of α-crystallin with unfolded proteins

1995 • 102 citations

Structural alterations of α‐crystallin during its chaperone action

1998 • 101 citations

Monodisperse Hsp16.3 Nonamer Exhibits Dynamic Dissociation and Reassociation, with the Nonamer Dissociation Prerequisite for Chaperone-like Activity

2002 • 101 citations

Unique Structural Features of a Novel Class of Small Heat Shock Proteins

1997 • 98 citations

Small heat shock proteins from extremophiles: a review

2004 • 94 citations

Structural and Functional Changes in the αA-Crystallin R116C Mutant in Hereditary Cataracts

2000 • 94 citations

A critical motif for oligomerization and chaperone activity of bacterial α‐heat shock proteins

2002 • 94 citations

Site-directed mutations within the core “α-crystallin” domain of the small heat-shock protein, human αB-crystallin, decrease molecular chaperone functions

1999 • 93 citations

Site-directed Spin Labeling Study of Subunit Interactions in the α-Crystallin Domain of Small Heat-shock Proteins

1999 • 89 citations

Chaperone Activity and Homo- and Hetero-oligomer Formation of Bacterial Small Heat Shock Proteins

2000 • 87 citations

The molecular chaperone α-crystallin is in kinetic competition with aggregation to stabilize a monomeric molten-globule form of α-lactalbumin

2001 • 87 citations

Heat stress-induced localization of small heat shock proteins in mouse myoblasts: intranuclear lamin A/C speckles as target for αB-crystallin and Hsp25

2004 • 84 citations

Defined sequence segments of the small heat shock proteins HSP25 and αB‐crystallin inhibit actin polymerization

2001 • 84 citations

Role of the Conserved SRLFDQFFG Region of α-Crystallin, a Small Heat Shock Protein

2003 • 83 citations

HSP27 expression regulates CCK-induced changes of the actin cytoskeleton in CHO-CCK-A cells

1999 • 80 citations

Self-association of a Small Heat Shock Protein

2004 • 80 citations

Caenorhabditis elegans small heat‐shock proteins Hsp12.2 and Hsp12.3 form tetramers and have no chaperone‐like activity

1998 • 77 citations

Interaction of human recombinant αA‐ and αB‐crystallins with early and late unfolding intermediates of citrate synthase on its thermal denaturation

2001 • 76 citations

The R116C Mutation in αA-crystallin Diminishes Its Protective Ability against Stress-induced Lens Epithelial Cell Apoptosis

2002 • 75 citations

On the mechanism of chaperone activity of the small heat-shock protein ofMethanococcus jannaschii

2003 • 72 citations

Small heat shock protein p26 associates with nuclear lamins and HSP70 in nuclei and nuclear matrix fractions from stressed cells

2002 • 71 citations

Mimicking phosphorylation of the small heat‐shock protein αB‐crystallin recruits the F‐box protein FBX4 to nuclear SC35 speckles

2004 • 71 citations

Molecular Chaperones: Structure of a Protein Disaggregase

2004 • 68 citations

Distribution, phosphorylation, and activities of Hsp25 in heat-stressed H9c2 myoblasts: a functional link to cytoprotection

2002 • 67 citations

HSP16.6 Is Involved in the Development of Thermotolerance and Thylakoid Stability in the Unicellular Cyanobacterium, Synechocystis sp. PCC 6803

2000 • 67 citations

Mutants in a Small Heat Shock Protein That Affect the Oligomeric State

2004 • 67 citations

¹H NMR spectroscopy reveals that mouse Hsp25 has a flexible C‐terminal extension of 18 amino acids

1995 • 67 citations

Comparison of the homologous carboxy-terminal domain and tail of α-crystallin and small heat shock protein

1993 • 65 citations

αB-Crystallin-coated MAP microtubule resists nocodazole and calcium-induced disassembly

2004 • 64 citations

Nuclear speckle localisation of the small heat shock protein ?B-crystallin and its inhibition by the R120G cardiomyopathy-linked mutation

2003 • 64 citations

The small heat shock protein (HSP) 20 is dynamically associated with the actin cross-linking protein actinin

2003 • 62 citations

The Essential Role of the Flexible Termini in the Temperature-responsiveness of the Oligomeric State and Chaperone-like Activity for the Polydisperse Small Heat Shock Protein IbpB from Escherichia coli

2005 • 62 citations

Chaperone function of mutant versions of αA‐ and αB‐crystallin prepared to pinpoint chaperone binding sites

2001 • 60 citations

Oligomerization, Chaperone Activity, and Nuclear Localization of p26, a Small Heat Shock Protein from Artemia franciscana

2004 • 58 citations

Some properties of human small heat shock protein Hsp22 (H11 or HspB8)

2004 • 57 citations

Structural perturbation of α-crystallin and its chaperone-like activity

1998 • 56 citations

Mechanism of Chaperone Function in Small Heat-shock Proteins

2003 • 56 citations

Hsp90: from structure to phenotype

2004 • 55 citations

Phe71 Is Essential for Chaperone-like Function in αA-crystallin

2001 • 52 citations

Solution Structure and Dynamics of a Heat Shock Protein Assembly Probed by Hydrogen Exchange and Mass Spectrometry

2003 • 52 citations

Temperature-dependent subunit exchange and chaperone-like activities of Hsp16.3, a small heat shock protein from Mycobacterium tuberculosis

2004 • 51 citations

The Small Heat Shock Proteins of the Nematode Caenorhabditis elegans: Structure, Regulation and Biology

2002 • 50 citations

Influence of the C-Terminal Residues on Oligomerization of αA-Crystallin

2003 • 50 citations

The determinants of the oligomeric structure in Hsp16.5 are encoded in the α‐crystallin domain

2002 • 47 citations

Interaction of the small heat shock protein with molecular mass 25 kDa (hsp25) with actin

2003 • 46 citations

The αA-Crystallin R116C Mutant Has a Higher Affinity for Forming Heteroaggregates with αB-Crystallin

2001 • 46 citations

Diapause: diverse states of developmental and metabolic arrest

2005 • 45 citations

A small heat shock/α-crystallin protein from encysted Artemia embryos suppresses tubulin denaturation

2003 • 45 citations

Negative Charges in the C-terminal Domain Stabilize the αB-Crystallin Complex

1998 • 44 citations

Mutation of αB-crystallin: effects on chaperone-like activity

1998 • 42 citations

Native Quaternary Structure of Bovine α-Crystallin

2000 • 42 citations

HSP25, a Small Heat Shock Protein Associated with Dense Bodies and M-lines of Body Wall Muscle in Caenorhabditis elegans

2000 • 42 citations

Enhanced stability of αB-crystallin in the presence of small heat shock protein Hsp27

2003 • 42 citations

Nuclear staining for the small heat shock protein αB-crystallin colocalizes with splicing factor SC35

2003 • 42 citations

Small heat shock protein Hsp16.3 modulates its chaperone activity by adjusting the rate of oligomeric dissociation

2003 • 41 citations

HSP25 Is Involved in Two Steps of the Differentiation of PAM212 Keratinocytes

2004 • 40 citations

Identification of a Site of Hsp27 Binding with Hsp27 and αB-Crystallin as Indicated by the Yeast Two-Hybrid System

1999 • 39 citations

The small heat-shock chaperone protein, α-crystallin, does not recognise stable molten globule states of cytosolic proteins

2000 • 37 citations

Tomato heat stress protein Hsp16.1-CIII represents a member of a new class of nucleocytoplasmic small heat stress proteins in plants

2003 • 35 citations

The molecular chaperone α‐crystallin incorporated into red cell ghosts protects membrane Na/K‐ATPase against glycation and oxidative stress

2003 • 35 citations

Testis-specific human small heat shock protein HSPB9 is a cancer/testis antigen, and potentially interacts with the dynein subunit TCTEL1

2004 • 34 citations

Preheat Treatment for Mycobacterium tuberculosis Hsp16.3: Correlation between a Structural Phase Change at 60°C and a Dramatic Increase in Chaperone-like Activity

2001 • 31 citations

α-Crystallins are involved in specific interactions with the murine γD/E/F-crystallin-encoding gene

1994 • 31 citations

Stress protection by a fluorescent Hsp27 chimera that is independent of nuclear translocation or multimeric dissociation

2002 • 31 citations

αB-Crystallin phosphorylated at Ser-59 is localized in centrosomes and midbodies during mitosis

2001 • 30 citations

Structural Changes in α-Crystallin and Whole Eye Lens During Heating, Observed by Low-angle X-ray Diffraction

2004 • 29 citations

The association of small heat shock protein Hsp16.3 with the plasma membrane of Mycobacterium tuberculosis: Dissociation of oligomers is a prerequisite

2005 • 27 citations

Navigating the ClpB channel to solution

2005 • 27 citations

Probing alpha-crystallin structure using chemical cross-linkers and mass spectrometry.

2004 • 27 citations

Reversible methionine sulfoxidation of Mycobacterium tuberculosis small heat shock protein Hsp16.3 and its possible role in scavenging oxidants

2003 • 24 citations

N-terminal control of small heat shock protein oligomerization: changes in aggregate size and chaperone-like function

2005 • 24 citations

Three-dimensional models corresponding to the C-terminal domain of human αA- and αB-crystallins based on the crystal structure of the small heat-shock protein HSP16.9 from wheat

2003 • 23 citations

Mycobacterium tuberculosis Hsp16.3 Nonamers are Assembled and Re-assembled via Trimer and Hexamer Intermediates

2003 • 20 citations

Molecular characterization of Oryza sativa 16.9 kDa heat shock protein

1999 • 20 citations

C-terminal lysine truncation increases thermostability and enhances chaperone-like function of porcine αB-crystallin

2002 • 19 citations

Structural diversity in the small heat shock protein superfamily: control of aggregation by the N-terminal region

2003 • 17 citations

Bovine lens crystallins do contain helical structure: a circular dichroism study

1999 • 17 citations

The lack of chaperonelike activity of Caenorhabditis elegans Hsp12.2 cannot be restored by domain swapping with human αB-crystallin

2001 • 17 citations

Thermally induced disintegration of the oligomeric structure of alphaB-crystallin mutant F28S is associated with diminished chaperone activity.

2003 • 17 citations

Tsp36, a tapeworm small heat‐shock protein with a duplicated α‐crystallin domain, forms dimers and tetramers with good chaperone‐like activity

2004 • 15 citations

Effect of mutations of murine lens αB crystallin on transfected neural cell viability and cellular translocation in response to stress

1998 • 13 citations

Thermal stability of human α‐crystallins sensed by amide hydrogen exchange

2004 • 12 citations

Interaction of DNA with bovine lens α-crystallin: its functional implications

1998 • 10 citations

Quaternary Structure Of α-Crystallin Is Necessary For The Binding Of Unfolded Proteins: A Surface Plasmon Resonance Study

2004 • 10 citations

Two-dimensional crystallization of a small heat shock protein HSP16.3 on lipid layer

2003 • 8 citations