The conserved dinucleotide AG of the 3′ splice site may be recognized twice during in vitro splicing of mammalian mRNA precursors

Rapid and efficient site-specific mutagenesis without phenotypic selection.

1985 • 7,573 citations

Efficientin vitrosynthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter

1984 • 6,459 citations

Are snRNPs involved in splicing?

1980 • 1,206 citations

A compensatory base change in U1 snRNA suppresses a 5′ splice site mutation

1986 • 755 citations

Exon definition may facilitate splice site selection in RNAs with multiple exons.

1990 • 726 citations

The U1 small nuclear RNA-protein complex selectively binds a 5′ splice site in vitro

1983 • 593 citations

A mechanism for RNA splicing.

1980 • 553 citations

A factor, U2AF, is required for U2 snRNP binding and splicing complex assembly

1988 • 535 citations

Interactions between small nuclear ribonucleoprotein particles in formation of spliceosomes

1987 • 530 citations

Recognition of the TACTAAC box during mRNA splicing in yeast involves base pairing to the U2-like snRNA

1987 • 508 citations

Electrophoretic separation of complexes involved in the splicing of precursors to mRNAs

1986 • 490 citations

Spliceosomal RNA U6 is remarkably conserved from yeast to mammals

1988 • 428 citations

Spliceosome assembly in yeast.

1987 • 402 citations

Intron sequences involved in lariat formation during pre-mRNA splicing

1985 • 402 citations

Identification of functional U1 snRNA-pre-mRNA complexes committed to spliceosome assembly and splicing

1989 • 391 citations

A U1 snRNA:pre-mRNA base pairing interaction is required early in yeast spliceosome assembly but does not uniquely define the 5′ cleavage site.

1988 • 364 citations

Identification and purification of a 62,000-dalton protein that binds specifically to the polypyrimidine tract of introns.

1989 • 349 citations

Mammalian pre-mRNA branch site selection by U2 snRNP involves base pairing.

1989 • 314 citations

The organization of 3' splice-site sequences in mammalian introns.

1989 • 310 citations

The 3′ Splice Site of Pre-Messenger RNA Is Recognized by a Small Nuclear Ribonucleoprotein

1985 • 310 citations

5' splice site selection in yeast: genetic alterations in base-pairing with U1 reveal additional requirements.

1988 • 307 citations

A compensatory base change in human U2 snRNA can suppress a branch site mutation.

1989 • 284 citations

Electrophoresis of ribonucleoproteins reveals an ordered assembly pathway of yeast splicing complexes

1986 • 283 citations

A protein associated with small nuclear ribonucleoprotein particles recognizes the 3′ splice site of premessenger RNA

1986 • 283 citations

A protein that specifically recognizes the 3′ splice site of mammalian pre-mRNA introns is associated with a small nuclear ribonucleoprotein

1986 • 274 citations

An Early Hierarchic Role of U1 Small Nuclear Ribonucleoprotein in Spliceosome Assembly

1988 • 271 citations

RNA binding specificity of hnRNP proteins: a subset bind to the 3′ end of introns.

1988 • 251 citations

U4 and U6 RNAs coexist in a single small nuclear ribonucleoprotein particle

1984 • 238 citations

Mutually exclusive splicing of α-tropomyosin exons enforced by an unusual lariat branch point location: Implications for constitutive splicing

1989 • 238 citations

Cryptic branch point activation allows accurate in vitro splicing of human β-globin intron mutants

1985 • 236 citations

β+ Thalassemia: Aberrant splicing results from a single point mutation in an intron

1981 • 235 citations

Evidence for the existence of snRNAs U4 and U6 in a single ribonucleoprotein complex and for their association by intermolecular base pairing.

1984 • 213 citations

Spliceosome assembly involves the binding and release of U4 small nuclear ribonucleoprotein.

1988 • 188 citations

Gel electrophoretic isolation of splicing complexes containing U1 small nuclear ribonucleoprotein particles.

1988 • 183 citations

UACUAAC is the preferred branch site for mammalian mRNA splicing.

1989 • 180 citations

Identification of a yeast snRNP protein and detection of snRNP-snRNP interactions

1987 • 180 citations

A mutational analysis of spliceosome assembly: evidence for splice site collaboration during spliceosome formation.

1987 • 179 citations

Antisense probing of the human U4U6 snRNP with biotinylated 2′-OMe RNA oligonucleotides

1989 • 169 citations

Splice site selection and ribonucleoprotein complex assembly during in vitro pre-mRNA splicing.

1988 • 163 citations

Early commitment of yeast pre-mRNA to the spliceosome pathway.

1988 • 143 citations

Localization of a base-paired interaction between small nuclear RNAs U4 and U6 in intact U4/U6 ribonucleoprotein particles by psoralen cross-linking

1985 • 137 citations

The role of the polypyrimidine stretch at the SV40 early pre-mRNA 3′ splice site in alternative splicing.

1988 • 105 citations

Mammalian U2 snRNP has a sequence-specific RNA-binding activity.

1989 • 98 citations

Gel Electrophoretic Isolation of Splicing Complexes Containing U1 Small Nuclear Ribonucleoprotein Particles

1988 • 95 citations

Abnormally spliced messenger RNA in erythroid cells from patients with β+ thalassemia and monkey cells expressing a cloned β+-thalassemic gene

1982 • 91 citations

Unusual branch point selection in processing of human growth hormone pre-mRNA.

1988 • 72 citations

Mutational analysis of the interactions between u1 small nuclear rna and pre-mrna of yeast

1989 • 42 citations

Purification and RNA binding properties of a C-type hnRNP protein from HeLa cells.

1987 • 33 citations

Mechanisms of human β-globin pre-mRNA splicing

1985 • 2 citations