The role of the polypyrimidine stretch at the SV40 early pre-mRNA 3′ splice site in alternative splicing.

[57] Sequencing end-labeled DNA with base-specific chemical cleavages

1980 • 15,302 citations

Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei

1983 • 11,902 citations

Efficientin vitrosynthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter

1984 • 6,459 citations

Organization and Expression of Eucaryotic Split Genes Coding for Proteins

1981 • 4,730 citations

A catalogue of splice junction sequences

1982 • 3,861 citations

SV40-transformed simian cells support the replication of early SV40 mutants

1981 • 2,564 citations

Alternative RNA processing in calcitonin gene expression generates mRNAs encoding different polypeptide products

1982 • 2,114 citations

Mapping of RNA by a modification of the Berk-Sharp procedure: the 5′ termini of 15 S β-globin mRNA precursor and mature 10 S β-globin mRNA have identical map coordinates

1979 • 1,286 citations

[32] Oligonucleotide-directed mutagenesis of DNA fragments cloned into M13 vectors

1983 • 1,093 citations

Tissue-specific generation of two preprotachykinin mRNAs from one gene by alternative RNA splicing

1984 • 563 citations

Stepwise assembly of a pre-mRNA splicing complex requires U-snRNPs and specific intron sequences

1985 • 524 citations

Evidence for an intron-contained sequence required for the splicing of yeast RNA polymerase II transcripts

1983 • 396 citations

Sequence requirements for splicing of higher eukaryotic nuclear pre-mRNA

1986 • 389 citations

A single-base change at a splice site in a β0-thalassemic gene causes abnormal RNA splicing

1982 • 372 citations

Intron splicing: a conserved internal signal in introns of animal pre-mRNAs.

1984 • 342 citations

Point mutations identify the conserved, intron-contained TACTAAC box as an essential splicing signal sequence in yeast

1984 • 339 citations

The 3′ Splice Site of Pre-Messenger RNA Is Recognized by a Small Nuclear Ribonucleoprotein

1985 • 310 citations

An RNA Processing Activity That Debranches RNA Lariats

1985 • 300 citations

Multiple sites of alternative splicing of the rat fibronectin gene transcript.

1987 • 299 citations

A protein associated with small nuclear ribonucleoprotein particles recognizes the 3′ splice site of premessenger RNA

1986 • 283 citations

A protein that specifically recognizes the 3′ splice site of mammalian pre-mRNA introns is associated with a small nuclear ribonucleoprotein

1986 • 274 citations

Evidence for the biochemical role of an internal sequence in yeast nuclear mRNA introns: Implications for U1 RNA and metazoan mRNA splicing

1983 • 239 citations

Human CSF-1: gene structure and alternative splicing of mRNA precursors.

1987 • 238 citations

Cryptic branch point activation allows accurate in vitro splicing of human β-globin intron mutants

1985 • 236 citations

Unusual splice sites revealed by mutagenic inactivation of an authentic splice site of the rabbit β-globin gene

1983 • 235 citations

Pre-mRNA splicing in vitro requires intact U4/U6 small nuclear ribonucleoprotein

1986 • 222 citations

U1, U2, and U4/U6 small nuclear ribonucleoproteins are required for in vitro splicing but not polyadenylation

1986 • 192 citations

Role of the 3′ splice site consensus sequence in mammalian pre-mRNA splicing

1985 • 184 citations

Efficient site-directed mutagenesis by simultaneous use of two primers

1983 • 154 citations

Multiple interactions between the splicing substrate and small nuclear ribonucleoproteins in spliceosomes.

1987 • 143 citations

Factors influencing alternative splice site utilization in vivo.

1987 • 120 citations

Sequence and structure of the dopa decarboxylase gene of Drosophila: evidence for novel RNA splicing variants.

1986 • 115 citations

Spontaneous splicing mutations at the dihydrofolate reductase locus in Chinese hamster ovary cells.

1986 • 109 citations

The role of the polypyrimidine stretch at the SV40 early pre-mRNA 3′ splice site in alternative splicing.

1988 • 105 citations

Alternative RNA Splicing Generates Transcripts Encoding a Thorax-Specific Isoform of Drosophila melanogaster Myosin Heavy Chain

1986 • 103 citations

Regulated splicing produces different forms of dopa decarboxylase in the central nervous system and hypoderm of Drosophila melanogaster.

1986 • 102 citations

Developmental variations in the splicing pattern of transcripts from the Drosophila gene encoding myosin alkali light chain result in different carboxyl-terminal amino acid sequences.

1985 • 101 citations

Splicing pathways of SV40 mRNAs in X. laevis oocytes differ in their requirements for snRNPs

1984 • 98 citations

Nonconsensus branch-site sequences in the in vitro splicing of transcripts of mutant rabbit beta-globin genes.

1985 • 96 citations

Splicing of SV40 early pre-mRNA to large T and small t mRNAs utilizes different patterns of lariat branch sites

1987 • 93 citations

Multiple Interactions between the Splicing Substrate and Small Nuclear Ribonucleoproteins in Spliceosomes

1987 • 86 citations

The natural 5' splice site of simian virus 40 large T antigen can be improved by increasing the base complementarity to U1 RNA.

1987 • 84 citations

The microvillus 110K cytoskeletal protein is an integral membrane protein

1984 • 66 citations

Repression of simian virus 40 early transcription by viral DNA replication in human 293 cells

1985 • 65 citations

Factors Influencing Alternative Splice Site Utilization In Vivo

1987 • 48 citations

Alternative branch points are selected during splicing of a yeast pre-mRNA in mammalian and yeast extracts.

1986 • 48 citations

In vitrosplicing of simian virus 40 early pre mRNA

1986 • 48 citations

mRNA precursor splicing in vivo: sequence requirements determined by deletion analysis of an intervening sequence.

1985 • 36 citations

The Natural 5′ Splice Site of Simian Virus 40 Large T Antigen can be improved by increasing the base Complementarity to U1 RNA

1987 • 29 citations

Splicing of the adenovirus-2 E1A 13S mRNA requires a minimal intron length and specific intron signals

1985 • 25 citations