Prohormone-Converting Enzymes: Regulation and Evaluation of Function Using Antisense RNA

Single-Step Method of RNA Isolation by Acid Guanidinium Thiocyanate–Phenol–Chloroform Extraction

1987 • 64,270 citations

Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease

1979 • 22,482 citations

A simple method for displaying the hydropathic character of a protein

1982 • 22,471 citations

Point mutations define a sequence flanking the AUG initiator codon that modulates translation by eukaryotic ribosomes

1986 • 4,785 citations

A new method for predicting signal sequence cleavage sites

1986 • 4,629 citations

Characterization of a 41-Residue Ovine Hypothalamic Peptide that Stimulates Secretion of Corticotropin and β-Endorphin

1982 • 3,896 citations

The signal peptide

1990 • 1,096 citations

Mammalian subtilisins: The long-sought dibasic processing endoproteases

1991 • 756 citations

PC1 and PC2 are proprotein convertases capable of cleaving proopiomelanocortin at distinct pairs of basic residues.

1991 • 589 citations

Identification of a human insulinoma cDNA encoding a novel mammalian protein structurally related to the yeast dibasic processing protease Kex2.

1990 • 579 citations

Downstream secondary structure facilitates recognition of initiator codons by eukaryotic ribosomes.

1990 • 550 citations

Cloning and Primary Sequence of a Mouse Candidate Prohormone Convertase PC1 Homologous to PC2, Furin, and Kex2: Distinct Chromosomal Localization and Messenger RNA Distribution in Brain and Pituitary Compared to PC2

1991 • 475 citations

Identification of a cDNA encoding a second putative prohormone convertase related to PC2 in AtT20 cells and islets of Langerhans.

1991 • 449 citations

Intracellular Targeting and Structural Conservation of a Prohormone-Processing Endoprotease

1989 • 395 citations

How the messenger got its tail: addition of poly(A) in the nucleus

1990 • 390 citations

Yeast prohormone processing enzyme (KEX2 gene product) is a Ca2+-dependent serine protease.

1989 • 386 citations

Human fur gene encodes a yeast KEX2-like endoprotease that cleaves pro-beta-NGF in vivo.

1990 • 378 citations

Kex2-like endoproteases PC2 and PC3 accurately cleave a model prohormone in mammalian cells: evidence for a common core of neuroendocrine processing enzymes.

1991 • 317 citations

Expression of a human proprotein processing enzyme: correct cleavage of the von Willebrand factor precursor at a paired basic amino acid site.

1990 • 296 citations

Furin is a subtilisin-like proprotein processing enzyme in higher eukaryotes

1990 • 259 citations

Structure and expression of mouse furin, a yeast Kex2-related protease. Lack of processing of coexpressed prorenin in GH4C1 cells.

1990 • 219 citations

Structural homology between the human fur gene product and the subtilisin-like protease encoded by yeast KEX2

1990 • 202 citations

Yeast KEX2 Endopeptidase Correctly Cleaves a Neuroendocrine Prohormone in Mammalian Cells

1988 • 173 citations

Proteolytic processing in the biosynthesis of insulin and other proteins.

1974 • 172 citations

Structure of the Precursor to an Enzyme Mediating COOH-Terminal Amidation in Peptide Biosynthesis

1987 • 163 citations

Clathrin: A Role in the Intracellular Retention of a Golgi Membrane Protein

1989 • 154 citations

Simplified northern blot hybridization using 5% sodium dodecyl sulfate.

1990 • 149 citations

fur gene expression as a discriminating marker for small cell and nonsmall cell lung carcinomas.

1987 • 144 citations

Alternative mRNA splicing generates multiple forms of peptidyl-glycine alpha-amidating monooxygenase in rat atrium.

1989 • 116 citations

The Mechanism of Action of Nerve Growth Factor

1991 • 102 citations

Coordinate, equimolar secretion of smaller peptide products derived from pro-ACTH/endorphin by mouse pituitary tumor cells.

1981 • 98 citations

Cloning and Functional Expression of a Novel Endoprotease Involved in Prohormone Processing at Dibasic Sites1

1991 • 96 citations

Characterization of PC2, a mammalian Kex2 homologue, following expression of the cDNA in microinjected Xenopus oocytes

1991 • 77 citations

Subtilisin-like proteinases involved in the activation of proproteins of the eukaryotic secretory pathway

1990 • 74 citations

Sequence of the cDNA encoding rat furin, a possible propeptide-processing endoprotease

1990 • 55 citations

In vitro mutagenesis of trypsinogen: role of the amino terminus in intracellular protein targeting to secretory granules.

1987 • 54 citations

Cell-Type Specific Posttranslational Processing of Peptides by Different Pituitary Cell Lines*

1990 • 49 citations

Neuropeptide Y (NPY) in pc12 phaeochromocytoma cultures: Responses to dexamethasone and nerve growth factor

1984 • 45 citations

Coordinate Regulation of Peptide Acetyltransferase Activity and Proopiomelanocortin Gene Expression in the Intermediate Lobe of the Rat Pituitary*

1986 • 45 citations

Positive regulation of proopiomelanocortin gene expression in corticotropes and melanotropes

1991 • 44 citations

The tissue-specific processing of Pro-ACTH/Endorphin recent advances and unsolved problems

1990 • 42 citations

Steroidogenic activity of high molecular weight forms of corticotropin

1979 • 41 citations

Manipulation of Neuropeptide Biosynthesis through the Expression of Antisense RNA for Peptidylglycine α-Amidating Monooxygenase

1991 • 39 citations

Endoproteolytic processing of the human protein C precursor by the yeast Kex2 endopeptidase coexpressed in mammalian cells

1991 • 39 citations

Effect of Secretagogues on Components of the Secretory System in AtT-20 Cells*

1990 • 36 citations

Immunocytochemical and in Situ Hybridization Studies of Peptidylglycine α-Amidating Monooxygenase in Pituitary Gland*

1990 • 31 citations

Partial processing of the neuropeptide Y precursor in transfected CHO cells

1990 • 30 citations

Investigation of the Structural Requirements for Peptide Precursor Processing in AtT-20 Cells Using Site-Directed Mutagenesis of Proadrenocorticotropin/Endorphin

1991 • 25 citations

Prorenin is sorted into the regulated secretory pathway independent of its processing to renin in mouse pituitary AtT‐20 cells

1989 • 24 citations

How Peptide Hormones Get Ready for Work

1991 • 23 citations

Characterization of mouse tumor cell beta-lipotropin.

1979 • 21 citations

Functional diversity of bioactive peptides in the nervous system itself: “How the brain may understand”

1989 • 8 citations

Expression and Sorting of Rat Plasma Kallikrein in POMC-Producing AtT-20 Cells

1991 • 7 citations

Prohormone-Converting Enzymes

1986 • 6 citations

Homology cloning of aspartic proteases from an endocrine cell line using the polymerase chain reaction

1991 • 5 citations

Deleted Work

1955 • 0 citations