Expression of a human proprotein processing enzyme: correct cleavage of the von Willebrand factor precursor at a paired basic amino acid site.

Improved tools for biological sequence comparison.

1988 • 11,455 citations

Enzymatic Amplification of β-Globin Genomic Sequences and Restriction Site Analysis for Diagnosis of Sickle Cell Anemia

1985 • 9,128 citations

Transfer of proteins across membranes. I. Presence of proteolytically processed and unprocessed nascent immunoglobulin light chains on membrane-bound ribosomes of murine myeloma.

1975 • 3,026 citations

The molecular basis of blood coagulation

1988 • 1,240 citations

Serine Proteases: Structure and Mechanism of Catalysis

1977 • 1,238 citations

Calcium phosphate-mediated gene transfer: a highly efficient transfection system for stably transforming cells with plasmid DNA.

1988 • 897 citations

How signal sequences maintain cleavage specificity

1984 • 725 citations

THE BIOSYNTHESIS OF INSULIN AND A PROBABLE PRECURSOR OF INSULIN BY A HUMAN ISLET CELL ADENOMA

1967 • 608 citations

Endoproteolytic cleavage of gp160 is required for the activation of human immunodeficiency virus

1988 • 608 citations

Post-Translational Proteolysis in Polypeptide Hormone Biosynthesis

1982 • 590 citations

Identification of a human insulinoma cDNA encoding a novel mammalian protein structurally related to the yeast dibasic processing protease Kex2.

1990 • 579 citations

POLYPROTEIN GENE EXPRESSION: Generation of Diversity of Neuroendocrine Peptides

1984 • 497 citations

Intracellular Targeting and Structural Conservation of a Prohormone-Processing Endoprotease

1989 • 395 citations

The Phosphorylation State of Eucaryotic Initiation Factor 2 Alters Translational Efficiency of Specific mRNAs

1989 • 393 citations

Yeast prohormone processing enzyme (KEX2 gene product) is a Ca2+-dependent serine protease.

1989 • 386 citations

Ligand-affinity cloning and structure of a cell surface heparan sulfate proteoglycan that binds basic fibroblast growth factor.

1990 • 274 citations

Yeast KEX2 gene encodes an endopeptidase homologous to subtilisin-like serine proteases

1988 • 236 citations

Secretion and processing of insulin precursors in yeast.

1986 • 227 citations

Type 1 Transforming Growth Factor Beta: Amplified Expression and Secretion of Mature and Precursor Polypeptides in Chinese Hamster Ovary Cells

1987 • 224 citations

Structure of pre-pro-von Willebrand factor and its expression in heterologous cells

1986 • 212 citations

Evolutionary conserved close linkage of the c-fes/fps proto-oncogene and genetic sequences encoding a receptor-like protein.

1986 • 207 citations

Structural homology between the human fur gene product and the subtilisin-like protease encoded by yeast KEX2

1990 • 202 citations

The propeptide of rat bone gamma-carboxyglutamic acid protein shares homology with other vitamin K-dependent protein precursors.

1985 • 195 citations

The propeptide of von Willebrand Factor independently mediates the assembly of von Willebrand multimers

1988 • 192 citations

Intracellular processing of the gp160 HIV-1 envelope precursor. Endoproteolytic cleavage occurs in a cis or medial compartment of the Golgi complex.

1990 • 190 citations

Yeast KEX2 Endopeptidase Correctly Cleaves a Neuroendocrine Prohormone in Mammalian Cells

1988 • 173 citations

Isolation of a Glucagon-containing Peptide: Primary Structure of a Possible Fragment of Proglucagon

1973 • 165 citations

Protease B of the lysosomelike vacuole of the yeast Saccharomyces cerevisiae is homologous to the subtilisin family of serine proteases.

1987 • 161 citations

Propolypeptide of von Willebrand Factor Circulates in Blood and Is Identical to von Willebrand Antigen II

1986 • 160 citations

Nucleotide sequence of pre-pro-von Willebrand factor cDNA

1986 • 152 citations

Processing of the native nerve growth factor precursor to form biologically active nerve growth factor.

1988 • 139 citations

Molecular Genetic Neuroscience

1983 • 121 citations

The Secretion of Human Serum Albumin from the Yeast Saccharomyces cerevisiae Using Five Different Leader Sequences

1990 • 117 citations

Hormone processing and membrane-bound proteinases in yeast.

1985 • 114 citations

Cloning of three human multifunctional de novo purine biosynthetic genes by functional complementation of yeast mutations.

1990 • 110 citations

Amino-terminal sequences of prosomatostatin direct intracellular targeting but not processing specificity

1989 • 108 citations

Human pituitary tryptase: molecular forms, NH2-terminal sequence, immunocytochemical localization, and specificity with prohormone and fluorogenic substrates.

1987 • 99 citations

Expression of preprosomatostatin in heterologous cells: Biosynthesis, posttranslational processing, and secretion of mature somatostatin

1984 • 96 citations

Characterization of human c-fes/fps reveals a new transcription unit (fur) in the immediately upstream region of the proto-oncogene

1986 • 94 citations

Yeast KEX2 Protease Has the Properties of a Human Proalbumin Converting Enzyme

1987 • 85 citations

Purification and characterization of a paired basic residue-specific prohormone-converting enzyme from bovine pituitary neural lobe secretory vesicles.

1986 • 85 citations

Recombinant polypeptide from the endonuclease region of the acquired immune deficiency syndrome retrovirus polymerase (pol) gene detects serum antibodies in most infected individuals

1986 • 71 citations

[44] Analysis of synthesis, processing, and secretion of proteins expressed in mammalian cells

1990 • 65 citations

Endoproteolytic processing of the dibasic cleavage site in the human protein C precursor in transfected mammalian cells: effects of sequence alterations on efficiency of cleavage

1990 • 60 citations

Gene Transfer Techniques to Study Neuropeptide Processing

1988 • 55 citations

Identification of a 31,500 molecular weight islet cell protease as cathepsin B.

1983 • 52 citations

Biosynthesis and posttranslational processing of site-directed endoproteolytic cleavage mutants of pro-neuropeptide Y in mouse pituitary cells.

1990 • 44 citations

Expression of Mouse Proopiomelanocortin in an Insulinoma Cell Line

1989 • 38 citations

Protease B of the Lysosomelike Vacuole of the Yeast Saccharomyces Cerevisiae Is Homologous to the Subtilisin Family of Serine Proteases

1987 • 38 citations

Progress in Hemostasis and Thrombosis

1978 • 34 citations

Proalbumin is processed to serum albumin in COS-1 cells transfected with cDNA for rat albumin

1989 • 15 citations