How the messenger got its tail: addition of poly(A) in the nucleus

Methods in Enzymology , Vol

1966 • 26,261 citations

Steps in the processing of Ad2 mRNA: Poly(A)+ Nuclear sequences are conserved and poly(A) addition precedes splicing

1978 • 478 citations

Accurate cleavage and polyadenylation of exogenous RNA substrate

1985 • 334 citations

Four factors are required for 3'-end cleavage of pre-mRNAs.

1989 • 217 citations

Polyadenylation of mRNA precursors

1988 • 210 citations

3′ cleavage and polyadenylation of mRNA precursors in vitro requires a poly(A) polymerase, a cleavage factor, and a snRNP

1988 • 205 citations

Separation and characterization of a poly(A) polymerase and a cleavage/specificity factor required for pre-mRNA polyadenylation

1988 • 195 citations

An ordered pathway of assembly of components required for polyadenylation site recognition and processing.

1989 • 194 citations

The AAUAAA sequence is required both for cleavage and for polyadenylation of simian virus 40 pre-mRNA in vitro.

1986 • 147 citations

Compensatory mutations suggest that base-pairing with a small nuclear RNA is required to form the 3′ end of H3 messenger RNA

1986 • 146 citations

A small nuclear ribonucleoprotein associates with the AAUAAA polyadenylation signal in vitro

1986 • 139 citations

RNA Processing Generates the Mature 3′ End of Yeast CYC1 Messenger RNA in Vitro

1988 • 129 citations

Enzymes of Nucleic Acid Synthesis and Modification

2018 • 116 citations

Poly(A) polymerase purified from HeLa cell nuclear extract is required for both cleavage and polyadenylation of pre-mRNA in vitro.

1989 • 104 citations

Two phases in the addition of a poly(A) tail.

1989 • 98 citations

RNA sequence containing hexanucleotide AAUAAA directs efficient mRNA polyadenylation in vitro.

1985 • 95 citations

Analysis of RNA cleavage at the adenovirus-2 L3 polyadenylation site.

1986 • 94 citations

Functional analysis of point mutations in the AAUAAA motif of the SV40 late polyadenylation signal

1989 • 89 citations

RNA Processing In Vitro Produces Mature 3′ Ends of a Variety of Saccharomyces cerevisiae mRNAs

1990 • 84 citations

A beginning to the biochemistry of polyadenylation

1988 • 84 citations

Analysis of mRNA 3′ end formation by modification interference: the only modifications which prevent processing lie in AAUAAA and the poly(A) site.

1987 • 77 citations

Multiple forms of poly(A) polymerases purified from HeLa cells function in specific mRNA 3'-end formation.

1989 • 65 citations

Products of in vitro cleavage and polyadenylation of simian virus 40 late pre-mRNAs.

1987 • 64 citations

A Poly(A) Polymerase from Calf Thymus

1973 • 61 citations

The enzyme that adds poly(A) to mRNAs is a classical poly(A) polymerase.

1990 • 42 citations

RNA processing in vitro produces mature 3' ends of a variety of Saccharomyces cerevisiae mRNAs.

1990 • 37 citations

Role of poly(A) polymerase in the cleavage and polyadenylation of mRNA precursor.

1989 • 36 citations

Polyadenylation of mRNA: minimal substrates and a requirement for the 2' hydroxyl of the U in AAUAAA.

1990 • 35 citations

RNA 3'-end formation

1989 • 20 citations

Polyadenylation-specific complexes undergo a transition early in the polymerization of a poly(A) tail.

1990 • 12 citations