Variability in Caco-2 and MDCK Cell-Based Intestinal Permeability Assays

Characterization of the Human Colon Carcinoma Cell Line (Caco-2) as a Model System for Intestinal Epithelial Permeability

1989 • 2,127 citations

Correlation between oral drug absorption in humans and apparent drug permeability coefficients in human intestinal epithelial (Caco-2) cells

1991 • 1,787 citations

Enterocyte-like differentiation and polarization of the human colon carcinoma cell line Caco-2 in culture

1983 • 1,765 citations

One Hundred and Twenty-Seven Cultured Human Tumor Cell Lines Producing Tumors in Nude Mice23

1977 • 1,556 citations

Caco-2 monolayers in experimental and theoretical predictions of drug transport1PII of original article: S0169-409X(96)00415-2. The article was originally published in Advanced Drug Delivery Reviews 22 (1996) 67–84.1

2001 • 1,479 citations

The Caco-2 cell line as a model of the intestinal barrier: influence of cell and culture-related factors on Caco-2 cell functional characteristics

2005 • 1,317 citations

MDCK (Madin-Darby Canine Kidney) Cells: A Tool for Membrane Permeability Screening

1999 • 895 citations

Polarized monolayers formed by epithelial cells on a permeable and translucent support

1978 • 869 citations

Epithelial Transport Of Drugs In Cell Culture. I: A Model For Studying The Passive Diffusion Of Drugs Over Intestinal Absorbtive (Caco-2) Cells

1990 • 820 citations

Caco-2 cell monolayers as a model for drug transport across the intestinal mucosa.

1990 • 565 citations

Optimized conditions for prediction of intestinal drug permeability using Caco-2 cells

2000 • 533 citations

Transepithelial transport in cell culture.

1976 • 499 citations

Epithelial polarity, villin expression, and enterocytic differentiation of cultured human colon carcinoma cells: a survey of twenty cell lines.

1988 • 458 citations

Correlation of gene expression of ten drug efflux proteins of the ATP-binding cassette transporter family in normal human jejunum and in human intestinal epithelial Caco-2 cell monolayers.

2001 • 438 citations

A Human Colonic Cell Line Sharing Similarities With Enterocytes as a Model to Examine Oral Absorption: Advantages and Limitations of the Caco-2 Model

1997 • 433 citations

The human intestinal epithelial cell line Caco-2; pharmacological and pharmacokinetic applications

1995 • 404 citations

Functional expression of P-glycoprotein in apical membranes of human intestinal Caco-2 cells. Kinetics of vinblastine secretion and interaction with modulators

1993 • 365 citations

Regional levels of drug transporters along the human intestinal tract: Co-expression of ABC and SLC transporters and comparison with Caco-2 cells

2006 • 360 citations

Epithelial properties of human colonic carcinoma cell line Caco-2: electrical parameters

1984 • 349 citations

The Influence of Culture Time and Passage Number on the Morphological and Physiological Development of Caco-2 Cells

1997 • 295 citations

The use of cultured epithelial and endothelial cells for drug transport and metabolism studies.

1990 • 272 citations

Identification of two strains of MDCK cells which resemble separate nephron tubule segments

1981 • 269 citations

Caco-2 monolayers in experimental and theoretical predictions of drug transport

1996 • 268 citations

Comparison between active and passive drug transport in human intestinal epithelial (caco-2) cells in vitro and human jejunum in vivo

1996 • 259 citations

Correlation between oral drug absorption in humans, and apparent drug permeability in TC-7 cells, a human epithelial intestinal cell line: comparison with the parental Caco-2 cell line.

1998 • 239 citations

Cell cultures as models for drug absorption across the intestinal mucosa.

1991 • 229 citations

Uptake of the cephalosporin, cephalexin, by a dipeptide transport carrier in the human intestinal cell line, Caco-2

1990 • 224 citations

P-Glycoprotein (P-gp) Mediated Efflux in Caco-2 Cell Monolayers: The Influence of Culturing Conditions and Drug Exposure on P-gp Expression Levels

1998 • 221 citations

Transient mosaic patterns of morphological and functional differentiation in the Caco-2 cell line

1992 • 219 citations

Comparative studies of drug-metabolizing enzymes in dog, monkey, and human small intestines, and in Caco-2 cells.

1996 • 204 citations

Common characteristics for Na+-dependent sugar transport in Caco-2 cells and human fetal colon

1987 • 194 citations

Transepithelial Transport of Drugs by the Multidrug Transporter in Cultured Madin-Darby Canine Kidney Cell Epithelia

1989 • 191 citations

Assessing the Absorption of New Pharmaceuticals

2001 • 189 citations

Estimation of the relative contribution of the transcellular and paracellular pathway to the transport of passively absorbed drugs in the Caco-2 cell culture model.

1997 • 188 citations

The Madin Darby canine kidney (MDCK) epithelial cell monolayer as a model cellular transport barrier.

1989 • 185 citations

In vitro systems for studying intestinal drug absorption

1995 • 178 citations

Caco-2 cells express a combination of colonocyte and enterocyte phenotypes

1998 • 177 citations

In vitro trans-monolayer permeability calculations: often forgotten assumptions

2003 • 174 citations

Drug Bioavailability: Estimation of Solubility, Permeability, Absorption and Bioavailability

2004 • 164 citations

Transport of a large neutral amino acid (phenylalanine) in a human intestinal epithelial cell line: Caco-2

1990 • 163 citations

Passive Diffusion of Weak Organic Electrolytes across Caco‐2 Cell Monolayers: Uncoupling the Contributions of Hydrodynamic, Transcellular, and Paracellular Barriers

1995 • 153 citations

Presence and differential expression of SGLT1, GLUT1, GLUT2, GLUT3 and GLUT5 hexose-transporter mRNAs in Caco-2 cell clones in relation to cell growth and glucose consumption

1994 • 151 citations

pH-Dependent passive and active transport of acidic drugs across Caco-2 cell monolayers

2005 • 144 citations

The use of the intestinal epithelial cell culture model, Caco-2, in pharmaceutical development

1996 • 138 citations

Transport of bile acids in a human intestinal epithelial cell line, Caco-2

1990 • 136 citations

Evidence for diminished functional expression of intestinal transporters in Caco-2 cell monolayers at high passages.

1997 • 122 citations

Age-dependent expression of P-glycoprotein gp170 in Caco-2 cell monolayers.

1996 • 119 citations

Characterization of the unstirred water layer in Caco-2 cell monolayers using a novel diffusion apparatus.

1991 • 119 citations

Drug adsorption to plastic containers and retention of drugs in cultured cells under in vitro conditions

2006 • 119 citations

Dipeptide transporters in apical and basolateral membranes of the human intestinal cell line Caco-2

1993 • 116 citations

Do cell culture conditions influence the carrier-mediated transport of peptides in Caco-2 cell monolayers?

2003 • 113 citations

pH-Dependent and Carrier-mediated Transport of Salicylic Acid Across Caco-2 Cells

1994 • 111 citations

Heterogeneity in the human intestinal cell line Caco-2 leads to differences in transepithelial transport

1995 • 95 citations

Development of a more rapid, reduced serum culture system for Caco-2 monolayers and application to the biopharmaceutics classification system

2000 • 94 citations

Functional characteristics and steroid hormone-mediated regulation of an organic cation transporter in Madin-Darby canine kidney cells.

2001 • 89 citations

Optimizing Caco-2 cell monolayers to increase throughput in drug intestinal absorption analysis

2001 • 86 citations

Use of a Caco-2 cell culture model for the characterization of intestinal absorption of antibiotics

1999 • 76 citations

Permeability characteristics of parental and clonal human intestinal Caco-2 cell lines differentiated in serum-supplemented and serum-free media

2003 • 74 citations

Mechanism of L-alpha-methyldopa transport through a monolayer of polarized human intestinal epithelial cells (Caco-2).

1990 • 73 citations

Transport Properties Are Not Altered across Caco-2 Cells with Heightened TEER Despite Underlying Physiological and Ultrastructural Changes

1996 • 69 citations

Apical to basolateral surface area ratio and polarity of MDCK cells grown on different supports

1992 • 67 citations

A new diffusion chamber system for the determination of drug permeability coefficients across the human intestinal epithelium that are independent of the unstirred water layer

1992 • 65 citations

MDCK cell cultures as an epithelial in vitro model: cytoskeleton and tight junctions as indicators for the definition of age-related stages by confocal microscopy.

1998 • 64 citations

Variation of Peptide Transporter (PepT1 and HPT1) Expression in Caco‐2 Cells as a Function of Cell Origin

2004 • 64 citations

Substrate specificity of the di/tripeptide transporter in human intestinal epithelia (Caco‐2): identification of substrates that undergo H⁺‐coupled absorption

1994 • 62 citations

Cultured monolayers of MDCK cells: A novel model system for the study of epithelial development and function

1982 • 61 citations

Nucleoside transport in human colonic epithelial cell lines: evidence for two Na+-independent transport systems in T84 and Caco-2 cells

1999 • 56 citations

Cell Culture Models of Biological Barriers: In vitro Test Systems for Drug Absorption and Delivery

2007 • 56 citations

Research Communication: Glutamine and Barrier Function in Cultured Caco-2 Epithelial Cell Monolayers

2003 • 55 citations

In vitro/in vivo models for peptide oral absorption: comparison of Caco-2 cell permeability with rat intestinal absorption of renin inhibitory peptides.

1993 • 53 citations

Selection of development candidates based on in vitro permeability measurements

1997 • 52 citations

Transport Processes in Pharmaceutical Systems

2001 • 51 citations

Functional Characterization of Monocarboxylic Acid, Large Neutral Amino Acid, Bile Acid and Peptide Transporters, and P-Glycoprotein in MDCK and Caco-2 Cells

2002 • 51 citations

Cell culture techniques for the study of drug transport

1990 • 50 citations

Influence of drugs and nutrients on transporter gene expression levels in Caco-2 and LS180 intestinal epithelial cell lines.

2003 • 49 citations

Quantitative Approaches to Delineate Passive Transport Mechanisms in Cell Culture Monolayers

1999 • 49 citations

In vitro permeability of eight β-blockers through Caco-2 monolayers utilizing liquid chromatography/electrospray ionization mass spectrometry

1998 • 48 citations

Carrier affinity as a mechanism for the pH-dependence of folate transport in the small intestine

1990 • 45 citations

Evaluation of cocktail approach to standardise Caco-2 permeability experiments

2006 • 44 citations

Functional expression of novel peptide transporter in renal basolateral membranes

2000 • 43 citations

Formation of multilayers in the caco-2 cell culture model: a confocal laser scanning microscopy study.

2000 • 42 citations

The MDCK cell line is made up of populations of cells with diverse resistive and transport properties

2000 • 41 citations

High glucose concentration in isotonic media alters Caco-2 cell permeability

2003 • 41 citations

Effect of experimental pH on the in vitro permeability in intact rabbit intestines and Caco-2 monolayer

2005 • 39 citations

In-depth evaluation of Gly-Sar transport parameters as a function of culture time in the Caco-2 cell model

2003 • 39 citations

H(+)-peptide cotransport in Madin-Darby canine kidney cells: expression and calmodulin-dependent regulation

1995 • 39 citations

The transport of acidic amino acids and their analogues across monolayers of human intestinal absorptive (Caco-2) cells in vitro

1995 • 35 citations

Influence of the Microporous Substratum and Hydrodynamics on Resistances to Drug Transport in Cell Culture Systems: Calculation of Intrinsic Transport Parameters

1997 • 34 citations

Variability of functional characteristics of MDCK cells

1986 • 34 citations

Changes of Proliferative Activity and Phenotypes in Spontaneous Differentiation of a Colon Cancer Cell Line

1993 • 33 citations

Migration of polarized epithelial cells through permeable membrane substrates of defined pore size.

1992 • 30 citations

Comparison of Uptake Characteristics of Thymidine and Zidovudine in a Human intestinal Epithelial Model System

1993 • 29 citations

The Effect of Cell Culture Conditions on Saquinavir Transport Through, and Interactions with, MDCKII Cells Overexpressing hMDR1

2003 • 28 citations

Permeable support type influences the transport of compounds across Caco-2 cells

1992 • 26 citations

Sulfate conjugating and transport functions of MDCK distal tubular cells

2003 • 25 citations

Biotin transport in a human intestinal epithelial cell line (Caco-2)

1993 • 23 citations

Validation of cell culture models for the intestine and the blood-brain barrier and comparison of drug permeation.

2004 • 20 citations

Transepithelial secretion, cellular accumulation and cytotoxicity of vinblastine in defined MDCK cell strains

1993 • 20 citations

Extracellular Glucose Concentration Alters Functional Activity of the Intestinal Oligopeptide Transporter (PepT-1) in Caco-2 Cells

2003 • 19 citations

Alteration of culture regime modifies antioxidant defenses independent of intracellular reactive oxygen levels and resistance to severe oxidative stress within confluent Caco-2 "intestinal cells".

2001 • 12 citations

Evaluation of tissue culture insert membrane compatibility in the fluorescein leakage assay

1997 • 12 citations

Phosphate transport and alkaline phosphatase in confluent MDCK cell monolayers

1987 • 9 citations

In vitro measurement of drug transport using a new diffusion chamber compatible with Millicell® culture supports: Performance with caco-2 monolayers

1996 • 8 citations

Rational Delivery Strategies for the Design of Peptides with Enhanced Oral Delivery

1994 • 8 citations

Implementation of the caco-2 cell culture model as a predictive tool for the oral absorption of drugs. In-house evaluation procedures.

2003 • 6 citations

The cytosolic glutathione S-transferase isoenzymes in the dog kidney cortex as compared with the corresponding MDCK renal cell line

1996 • 4 citations

Regulatory acceptance of in vitro permeability studies in the context of the biopharmaceutics classification system

2002 • 4 citations