Site-directed mutation of the Escherichia coli ada gene: effects of substitution of methyl acceptor cysteine-321 by histidine in Ada protein

Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

1970 • 253,849 citations

Molecular Cloning: A Laboratory Manual

2001 • 133,517 citations

DNA sequencing with chain-terminating inhibitors

1977 • 69,181 citations

Short-Term Effects of Nose-Only Cigarette Smoke Exposure on Glutathione Redox Homeostasis, Cytochrome P450 1A1/2 and Respiratory Enzyme Activities in Mice Tissues

2013 • 47,043 citations

Experiments in molecular genetics

1972 • 28,260 citations

A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity

1983 • 26,063 citations

“Western Blotting”: Electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A

1981 • 8,657 citations

Ribonucleic acid isolated by cesium chloride centrifugation

1974 • 2,263 citations

Efficient isolation of genes by using antibody probes.

1983 • 2,065 citations

Improved technique utilizing nonfat dry milk for analysis of proteins and nucleic acids transferred to nitrocellulose

1984 • 1,697 citations

PROTEIN-DNA RECOGNITION

1984 • 1,649 citations

[32] Oligonucleotide-directed mutagenesis of DNA fragments cloned into M13 vectors

1983 • 1,093 citations

Polymer support oligonucleotide synthesis XVIII^1.2): use ofβ-cyanoethyi-N,N-dialkylamino-/N-morpholino phosphoramidite of deoxynucleosides for the synthesis of DNA fragments simplifying deprotection and isolation of the final product

1984 • 840 citations

REGULATION AND EXPRESSION OF THE ADAPTIVE RESPONSE TO ALKYLATING AGENTS

1988 • 645 citations

Repair of alkylated DNA in Escherichia coli. Methyl group transfer from O6-methylguanine to a protein cysteine residue.

1980 • 438 citations

Rapid and easy sequencing of large linear double-stranded DNA and supercoiled plasmid DNA

1985 • 410 citations

The intracellular signal for induction of resistance to alkylating agents in E. coli

1986 • 222 citations

Active site and complete sequence of the suicidal methyltransferase that counters alkylation mutagenesis.

1985 • 221 citations

Repair of alkylated DNA in Escherichia coli. Physical properties of O6-methylguanine-DNA methyltransferase.

1982 • 172 citations

Isolation and characterization of Escherichia coli K-12 mutants unable to induce the adaptive response to simple alkylating agents

1979 • 160 citations

Demethylation of O6-methylguanine in a synthetic DNA polymer by an inducible activity in Escherichiacoli

1980 • 141 citations

DNA METHYLATION IN ESCHERICHIA COLI

1987 • 127 citations

Cloning of theE. coliO⁶-methylguanine and methylphosphotriester methyltransferase gene using a functional DNA repair assay

1985 • 124 citations

Purification and structure of the intact Ada regulatory protein of Escherichia coli K12, O6-methylguanine-DNA methyltransferase.

1985 • 124 citations

Molecular cloning of a gene which regulates the adaptive response to alkylating agents in Escherichia coli

1983 • 110 citations

Regulatory mechanisms for induction of synthesis of repair enzymes in response to alkylating agents: ada protein acts as a transcriptional regulator.

1986 • 108 citations

Functional domains and methyl acceptor sites of the Escherichia coli ada protein.

1988 • 107 citations

Sequences of theE. coli uvrCgene and protein

1984 • 100 citations

Induction and autoregulation of ada, a positively acting element regulating the response of Escherichia coli K-12 to methylating agents

1985 • 87 citations

Cyclic AMP-dependent initiation and rho-dependent termination of colicin E1 gene transcription.

1983 • 82 citations

How cyclic AMP and its receptor protein act in escherichia coli

1982 • 72 citations

Nonessentiality of histidine 291 of Rhodospirillum rubrum ribulose-bisphosphate carboxylase/oxygenase as determined by site-directed mutagenesis.

1986 • 71 citations

Regulatory regions of ColE1 that are involved in determination of plasmid copy number.

1983 • 70 citations

O6-methylguanine-DNA methyltransferase in wild-type and ada mutants of Escherichia coli

1982 • 70 citations

Sequences of theE. coli uvrBgene and protein

1986 • 63 citations

Functional sites of the Ada regulatory protein of Escherichia coli

1988 • 59 citations

Simplified DNA manupulations based on in vitro mutagenesis

1985 • 53 citations

Functional role of cysteine-146 in Escherichia coli thymidylate synthase.

1988 • 43 citations

Quantitation of O6-methylguanine-DNA methyltransferase in HeLa cells

1983 • 40 citations

Adaptive increase of O⁶-methlguanine-acceptor protein in HeLa cells following N-methyl-N′-nitro-N-nitrosoguanidine treatment

1982 • 38 citations

Cellular Defense Mechanisms Against Alkylation of DNA

1982 • 31 citations

MutantEscherichia coliAda proteins simultaneously defective in the repair of O⁶-metbylguanine and in gene activation

1986 • 28 citations

Rapid, large-scale purification and characterization of ‘Ada protein’ (0⁶methylguanine-DNA methyltransferase) ofE.coli

1988 • 18 citations

Proteolytic processing of the Ada protein that repairs DNA O6-methylguanine residues in E. coli

1987 • 16 citations

[27] Methylated lysines and 3-methylhistidine in myosin: Tissue and developmental differences

1984 • 13 citations

High-level expression of the cloned ada gene of Escherichia coli by deletion of its regulatory sequence

1988 • 5 citations

Deleted Work

1955 • 0 citations