Functional role of cysteine-146 in Escherichia coli thymidylate synthase.

DNA sequencing with chain-terminating inhibitors

1977 • 69,181 citations

Studies on transformation of Escherichia coli with plasmids

1983 • 11,179 citations

Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes

1986 • 6,284 citations

The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers

1982 • 6,153 citations

Amino acid analysis by reverse-phase high-performance liquid chromatography: Precolumn derivatization with phenylisothiocyanate

1984 • 1,616 citations

New Tn10 derivatives for transposon mutagenesis and for construction of lacZ operon fusions by transposition

1984 • 613 citations

Mutagenesis at a specific position in a DNA sequence.

1978 • 504 citations

Atomic Structure of Thymidylate Synthase: Target for Rational Drug Design

1987 • 329 citations

Cloning vectors that yield high levels of single-stranded DNA for rapid DNA sequencing

1984 • 238 citations

Functional Role of Aspartic Acid-27 in Dihydrofolate Reductase Revealed by Mutagenesis

1986 • 233 citations

Thymidylate synthetase: Mechanism of inhibition by 5-fluoro-2′-deoxyuridylate

1972 • 197 citations

Biochemical factors affecting the tightness of 5-fluorodeoxyuridylate binding to human thymidylate synthetase

1981 • 137 citations

Dihydrofolate reductase: thymidylate synthase, a bifunctional polypeptide from Crithidia fasciculata.

1980 • 130 citations

Factors affecting substrate binding in Lactobacillus casei thymidylate synthetase as studied by equilibrium dialysis

1976 • 112 citations

On the Mechanism of Action of Folate-And Biopterin-Requiring Enzymes

1980 • 103 citations

Primary structure of the Escherichia coli thyA gene and its thymidylate synthase product.

1983 • 97 citations

Rapid assay and purification of a unique signal peptidase that processes the prolipoprotein from Escherichia coli B.

1984 • 86 citations

Purification of mammalian tumor (L1210) thymidylate synthetase by affinity chromatography on stable biospecific adsorbent. Stabilization of the enzyme with neutral detergents.

1979 • 64 citations

[59] Separation of pteroyl-oligo-γ-l-glutamates by high-performance liquid chromatography

1980 • 47 citations

In vitro synthesis of alpha-carboxyl-linked folylpolyglutamates by an enzyme preparation from Escherichia coli.

1986 • 41 citations

The primary structure of Lactobacillus casei thymidylate synthetase. II. The complete amino acid sequence of the active site peptide, CNBr 4.

1979 • 41 citations

Sources of one-carbon units in the folate pathway of Escherichia coli.

1982 • 38 citations

Thymidylate synthetase and 2'-deoxyuridylate form a tight complex in the presence of pteroyltriglutamate.

1979 • 33 citations

Differential inhibition of host and viral thymidylate synthetases by folylpolyglutamates.

1979 • 31 citations

Trapping and partial characterization of an adduct postulated to be the covalent catalytic ternary complex of thymidylate synthase

1986 • 27 citations

Isolation of the thymidylate synthetase gene (TMP1) by complementation in Saccharomyces cerevisiae.

1982 • 26 citations

Interconvertible forms of Escherichia coli dihydrofolate reductase with different affinities for analogs of dihydrofolate.

1976 • 20 citations

Isolation of the Thymidylate Synthetase Gene (TMP1) by Complementation in Saccharomyces cerevisiae

1982 • 10 citations