[32] Oligonucleotide-directed mutagenesis of DNA fragments cloned into M13 vectors

Screening λgt Recombinant Clones by Hybridization to Single Plaques in Situ

1977 • 4,696 citations

Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencing

1980 • 3,476 citations

Prolonged incubation in calcium chloride improves the competence of Escherichia coli cells

1979 • 1,454 citations

Synthesis of deoxyoligonucleotides on a polymer support

1981 • 870 citations

Hybridization of synthetic oligodeoxyribonucleotides to Φ_X174 DNA: the effect of single base pair mismatch

1979 • 753 citations

Mutagenesis at a specific position in a DNA sequence.

1978 • 504 citations

The use of synthetic oligonucleotides as hybridization probes. II. Hybridization of oligonucleotides of mixed sequence to rabbit β-globin DNA

1981 • 473 citations

The structure of transposable yeast mating type loci

1980 • 439 citations

The Biochemistry of Mutagenesis

1976 • 421 citations

DNA sequence analysis: a general, simple and rapid method for sequencing large oligodeoxyribonucleotide fragments by mapping

1974 • 395 citations

Resolving the functions of overlapping viral genes by site-specific mutagenesis at a mRNA splice site

1982 • 353 citations

The Molecular Biology of the Yeast Saccharomyces

1983 • 329 citations

The sequence of the DNAs coding for the mating-type loci of saccharomyces cerevisiae

1981 • 286 citations

Control of cell type in yeast by the mating type locus

1981 • 259 citations

A fast and simple method for sequencing DNA cloned in the single-stranded bacteriophage M13

1979 • 257 citations

Transposable mating type genes in Saccharomyces cerevisiae

1979 • 237 citations

Cloning of influenza cDNA into M13: the sequence of the RNA segment encoding the A/PR/8/34 matrix protein

1980 • 236 citations

A position effect in the control of transcription at yeast mating type loci

1981 • 233 citations

Oligonucleotide directed mutagenesis of the human β-globin gene: a general method for producing specific point mutations in cloned DNA

1981 • 207 citations

MUTATIONS AFFECTING SEXUAL CONJUGATION AND RELATED PROCESSES IN SACCHAROMYCES CEREVISIAE. II. GENETIC ANALYSIS OF NONMATING MUTANTS

1974 • 196 citations

Rapid synthesis of oligodeoxyribonucleotides VI. Effident, mechanised synthesis of heptadecadeoxyribonucleotides by an improved solid phase phosphotriester route

1981 • 185 citations

Automated Synthesis of Gene Fragments

1981 • 171 citations

In vitro mutation analysis of the mating-type locus in yeast

1981 • 170 citations

Total synthesis of a human leukocyte interferon gene

1981 • 160 citations

Construction of a functional human suppressor tRNA gene: an approach to gene therapy for β-thalassaemia

1982 • 154 citations

Directed Deletion of a Yeast Transfer RNA Intervening Sequence

1980 • 142 citations

DIRECTED MUTAGENESIS

1981 • 142 citations

Synthesis of oligodeoxyribonucleotides on silica gel support

1981 • 130 citations

Site-specific mutagenesis using synthetic oligodeoxyribonucleotide primers: I. Optimum conditions and minimum oligodeoxyribonucleotide length

1979 • 117 citations

Construction and characterization of new coliphage M13 cloning vectors

1980 • 84 citations

Genetic Assay for Small Fragments of Bacteriophage φX174 Deoxyribonucleic Acid

1971 • 81 citations

Site-directed mutagenesis in DNA: Generation of point mutations in cloned β globin complementary DNA at the positions corresponding to amino acids 121 to 123

1978 • 78 citations

Site-specific mutagenesis using synthetic oligodeoxyribonucleotide primers: II. In vitro selection of mutant DNA

1979 • 62 citations

The base-pairing specificity of cellulose-pdT9

1975 • 60 citations

Site-specific mutagenesis using oligodeoxyribonucleotides: isolation of a phenotypically silent φX174 mutant, with a specific nucleotide deletion, at very high efficiency

1980 • 57 citations

Construction of viable and lethal mutations in the origin of bacteriophage φX174 using synthetic oligodeoxyribonucleotides

1981 • 55 citations

Synthesis and properties of oligonucleotide-cellulose columns

1972 • 51 citations

Chemical DNA Synthesis and Recombinant DNA Studies

1980 • 49 citations

Control of yeast cell type by the mating type locus

1981 • 46 citations

The use of synthetic oligodeoxyribonucleotides to produce specific deletions in the araBAD promoter of Escherichia coli B/r

1982 • 40 citations

Properties of oligonucleotide cellulose columns

1973 • 32 citations

Biological activity of ΦX174 replicative form DNA fragments

1970 • 31 citations

Oligonucleotide-directed mutagenesis of gene IX of bacteriophage M13

1982 • 30 citations

Thermal Elution of Complementary Sequences of Nucleic Acids from Cellulose Columns with Covalently Attached Oligonucleotides of Known Length and Sequence

1971 • 25 citations

Properties of oligodeoxynucleotides that determine priming activity with Escherichia coli deoxyribonucleic acid polymerase I

1973 • 24 citations