Signals for Protein Import into Organelles

A new method for predicting signal sequence cleavage sites

1986 • 4,629 citations

Patterns of Amino Acids near Signal‐Sequence Cleavage Sites

1983 • 2,290 citations

Domain structure of mitochondrial and chloroplast targeting peptides

1989 • 1,191 citations

The signal peptide

1990 • 1,096 citations

Mitochondrial targeting sequences may form amphiphilic helices.

1986 • 1,019 citations

Mitochondrial protein import

1989 • 820 citations

The ATPase activity of secA is regulated by acidic phospholipids, secY, and the leader and mature domains of precursor proteins

1990 • 551 citations

A chemically synthesized pre-sequence of an imported mitochondrial protein can form an amphiphilic helix and perturb natural and artificial phospholipid bilayers.

1986 • 493 citations

Correlation of competence for export with lack of tertiary structure of the mature species: A study in vivo of maltose-binding protein in E. coli

1986 • 473 citations

Mitochondrial protein import: Identification of processing peptidase and of PEP, a processing enhancing protein

1988 • 388 citations

A conserved cleavage‐site motif in chloroplast transit peptides

1990 • 376 citations

Many Random Sequences Functionally Replace the Secretion Signal Sequence of Yeast Invertase

1987 • 375 citations

The role of the transit peptide in the routing of precursors toward different chloroplast compartments

1986 • 344 citations

MOM19, an import receptor for mitochondrial precursor proteins

1989 • 334 citations

Cleavage-site motifs in mitochondrial targeting peptides

1990 • 326 citations

Transport of proteins into chloroplasts

1984 • 310 citations

Sequence of the leader peptidase gene of Escherichia coli and the orientation of leader peptidase in the bacterial envelope.

1983 • 307 citations

Survey of amino-terminal proteolytic cleavage sites in mitochondrial precursor proteins: leader peptides cleaved by two matrix proteases share a three-amino acid motif.

1989 • 303 citations

A signal sequence receptor in the endoplasmic reticulum membrane

1987 • 291 citations

Successive translocation into and out of the mitochondrial matrix: Targeting of proteins to the intermembrane space by a bipartite signal peptide

1987 • 275 citations

Amphiphilicity is essential for mitochondrial presequence function.

1988 • 274 citations

The triose phosphate-3-phosphoglycerate-phosphate translocator from spinach chloroplasts: nucleotide sequence of a full-length cDNA clone and import of the in vitro synthesized precursor protein into chloroplasts.

1989 • 274 citations

Modulation of Folding Pathways of Exported Proteins by the Leader Sequence

1988 • 243 citations

Conformations of Signal Peptides Induced by Lipids Suggest Initial Steps in Protein Export

1986 • 225 citations

Identification of a receptor for protein import into chloroplasts and its localization to envelope contact zones

1988 • 204 citations

MAS1, a gene essential for yeast mitochondrial assembly, encodes a subunit of the mitochondrial processing protease.

1988 • 181 citations

Net N-C charge imbalance may be important for signal sequence function in bacteria

1986 • 176 citations

Chloroplast transit peptides from the green alga Chlamydomonas reinhardtii share features with both mitochondrial and higher plant chloroplast presequences

1990 • 173 citations

The Mitochondrial Targeting function of Randomly Generated Peptide Sequences Correlates with Predicted Helical Amphiphilicity

1989 • 164 citations

Alteration of the amino terminus of the mature sequence of a periplasmic protein can severely affect protein export in Escherichia coli.

1988 • 163 citations

A thylakoid processing protease is required for complete maturation of the lumen protein plastocyanin

1986 • 158 citations

Physiological role during export for the retardation of folding by the leader peptide of maltose-binding protein.

1989 • 158 citations

Mitochondrial protein import

1989 • 131 citations

The reaction specificities of the thylakoidal processing peptidase and Escherichia coli leader peptidase are identical.

1989 • 124 citations

Two mitochondrial matrix proteases act sequentially in the processing of mammalian matrix enzymes.

1988 • 122 citations

Direct probing of the interaction between the signal sequence of nascent preprolactin and the signal recognition particle by specific cross-linking.

1987 • 119 citations

Targeting of proteins to the thylakoid lumen by the bipartite transit peptide of the 33 kd oxygen-evolving protein.

1989 • 114 citations

Specific recognition of the leader region of precursor proteins is required for the activation of translocation ATPase of Escherichia coli.

1989 • 107 citations

Induction of non-bilayer lipid structures by functional signal peptides.

1990 • 107 citations

Introduction of basic amino acid residues after the signal peptide inhibits protein translocation across the cytoplasmic membrane of Escherichia coli. Relation to the orientation of membrane proteins.

1988 • 100 citations

Maturation of Escherichia coli maltose-binding protein by signal peptidase I in vivo. Sequence requirements for efficient processing and demonstration of an alternate cleavage site.

1990 • 100 citations

Functional and Nonfunctional LamB Signal Sequences Can Be Distinguished by Their Biophysical Properties

1989 • 97 citations

Retardation of folding as a possible means of suppression of a mutation in the leader sequence of an exported protein.

1988 • 95 citations

High-affinity binding sites involved in the import of porin into mitochondria.

1987 • 92 citations

Incorporation of a synthetic mitochondrial signal peptide into charged and uncharged phospholipid monolayers

1986 • 90 citations

Penetration of the signal sequence of Escherichia coli PhoE protein into phospholipid model membranes leads to lipid-specific changes in signal peptide structure and alterations of lipid organization

1988 • 89 citations

Helix formation and stability in a signal sequence

1989 • 86 citations

Evidence that a Chloroplast Surface Protein Is Associated with a Specific Binding Site for the Precursor to the Small Subunit of Ribulose-1,5-Bisphosphate Carboxylase

1987 • 79 citations

Conformations and orientations of a signal peptide interacting with phospholipid monolayers

1989 • 78 citations

N-Terminal Half of a Mitochondrial Presequence Peptide Takes a Helical Conformation When Bound to Dodecylphosphocholine Micelles: A Proton Nuclear Magnetic Resonance Study

1989 • 77 citations

Substrate specificity of eukaryotic signal peptidase. Site-saturation mutagenesis at position -1 regulates cleavage between multiple sites in human pre (delta pro) apolipoprotein A-II.

1988 • 76 citations

Characterization of the interfacial behavior and structure of the signal sequence of Escherichia coli outer membrane pore protein PhoE.

1988 • 72 citations

Photocrosslinking demonstrates proximity of a 34 kDa membrane protein to different portions of preprolactin during translocation through the endoplasmic reticulum

1989 • 70 citations

A conservative amino acid substitution, arginine for lysine, abolishes export of a hybrid protein in Escherichia coli

1989 • 69 citations

Sequence and structural requirements of a mitochondrial protein import signal defined by saturation cassette mutagenesis.

1989 • 69 citations

A Subunit of Mammalian Signal Peptidase Is Homologous to Yeast SEC11 Protein

1989 • 69 citations

Illicit secretion of a cytoplasmic protein into the periplasm of Escherichia coli requires a signal peptide plus a portion of the cognate secreted protein

1989 • 66 citations

Mitochondrial targeting sequences why ‘non‐amphiphilic’ peptides may still be amphiphilic

1988 • 57 citations

Suppression of a signal sequence mutation by an amino acid substitution in the mature portion of the maltose-binding protein

1987 • 54 citations

Solubilization and characterization of yeast signal peptidase

1989 • 45 citations

Sorting signals

1989 • 34 citations

Identification of prolipoprotein signal peptidase and genomic organization of the lsp gene in Escherichia coli.

1985 • 34 citations

(3] Purification and characterization of leader peptidase from Escherichia coli

1983 • 31 citations