The role of the transit peptide in the routing of precursors toward different chloroplast compartments

Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

1970 • 253,849 citations

A rapid alkaline extraction procedure for screening recombinant plasmid DNA

1979 • 15,511 citations

Efficientin vitrosynthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter

1984 • 6,459 citations

Quantitative Film Detection of 3H and 14C in Polyacrylamide Gels by Fluorography

1975 • 4,693 citations

Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencing

1980 • 3,476 citations

A rapid boiling method for the preparation of bacterial plasmids

1981 • 3,334 citations

pEMBL: a new family of single stranded plasmids

1983 • 1,231 citations

An optimized freeze-squeeze method for the recovery of DNA fragments from agarose gels

1983 • 565 citations

Chloroplast Proteins: Synthesis, Transport, and Assembly

1981 • 424 citations

Post-translational transport into intact chloroplasts of a precursor to the small subunit of ribulose-1,5-bisphosphate carboxylase

1978 • 383 citations

Targeting of a foreign protein to chloroplasts by fusion to the transit peptide from the small subunit of ribulose 1,5-bisphosphate carboxylase

1985 • 327 citations

Transport of proteins into chloroplasts

1984 • 310 citations

Nucleotide sequences of two pea cDNA clones encoding the small subunit of ribulose 1,5-bisphosphate carboxylase and the major chlorophyll a/b-binding thylakoid polypeptide.

1983 • 307 citations

[31] Use of protein A—bearing staphylococci for the immunoprecipitation and isolation of antigens from cells

1981 • 307 citations

Precursors to two nuclear-encoded chloroplast proteins bind to the outer envelope membrane before being imported into chloroplasts.

1985 • 305 citations

The first twelve amino acids (less than half of the pre-sequence) of an imported mitochondrial protein can direct mouse cytosolic dihydrofolate reductase into the yeast mitochondrial matrix.

1985 • 279 citations

Genome Organization and Expression in Plants

1980 • 243 citations

A leader peptide is sufficient to direct mitochondrial import of a chimeric protein.

1985 • 239 citations

The use of nuclear-encoded sequences to direct the light-regulated synthesis and transport of a foreign protein into plant chloroplasts

1985 • 233 citations

The sites of synthesis of the principal thylakoid membrane polypeptides in Chlamydomonas reinhardtii.

1977 • 231 citations

Thermolysin Is a Suitable Protease for Probing the Surface of Intact Pea Chloroplasts

1984 • 230 citations

Structure and expression of a pea nuclear gene encoding a chlorophyll a/b-binding polypeptide

1984 • 217 citations

Molecular cloning and sequencing of cDNA encoding the precursor to the small subunit of chloroplast ribulose-1,5-bisphosphate carboxylase

1980 • 202 citations

The nucleotide sequence, expression, and evolution of one member of a multigene family encoding the small subunit of ribulose-1,5-bisphosphate carboxylase in soybean.

1982 • 195 citations

Separation and characterization of inner and outer envelope membranes of pea chloroplasts

1981 • 192 citations

[41] Preparation of a cell-free protein-synthesizing system from wheat germ

1983 • 169 citations

Isolation and characterization of chloroplast envelope membranes

1986 • 161 citations

The architecture of photosynthetic membranes: lateral and transverse organization

1982 • 149 citations

NH2-terminal amino acid sequences of precursor and mature forms of the ribulose-1,5-bisphosphate carboxylase small subunit from Chlamydomonas reinhardtii.

1979 • 142 citations

Sequence of the precursor of the chloroplast thylakoid lumen protein plastocyanin

1985 • 133 citations

The plant ferredoxin precursor: nucleotide sequence of a full length cDNA clone

1985 • 129 citations

A yeast mutant temperature-sensitive for mitochondrial assembly is deficient in a mitochondrial protease activity that cleaves imported precursor polypeptides.

1985 • 107 citations

Precursor proteins are transported into mitochondria in the absence of proteolytic cleavage of the additional sequences.

1983 • 95 citations

Processing of small subunit precursor of ribulose bisphosphate carboxylase and its assembly into whole enzyme are stromal events

1979 • 93 citations

Nucleotide sequence encoding the precursor of the small subunit of ribulose 1,5-bisphosphale carboxylase fromLemna gibbaL.G-3

1983 • 61 citations

Import of Polypeptides into Chloroplasts

1985 • 44 citations

In Vitro Synthesis, Transport, and Assembly of the Constituent Polypeptides of the Light-Harvesting Chlorophyll a/b Protein Complex

1980 • 35 citations