Mechanistically diverse enzyme superfamilies: the importance of chemistry in the evolution of catalysis

One thousand families for the molecular biologist

1992 • 1,023 citations

The crystal structure of urease from Klebsiella aerogenes

1995 • 795 citations

On the Evolution of Biochemical Syntheses

1945 • 595 citations

An evolutionary treasure: unification of a broad set of amidohydrolases related to urease

1997 • 491 citations

Evolutionary Divergence of Substrate Specificity within the Chymotrypsin-like Serine Protease Fold

1997 • 351 citations

Computer Analysis of Bacterial Haloacid Dehalogenases Defines a Large Superfamily of Hydrolases with Diverse Specificity

1994 • 310 citations

Crystal Structure of the Biphenyl-Cleaving Extradiol Dioxygenase from a PCB-Degrading Pseudomonad

1995 • 297 citations

The Enolase Superfamily: A General Strategy for Enzyme-Catalyzed Abstraction of the α-Protons of Carboxylic Acids

1996 • 297 citations

Understanding Enzyme Superfamilies

1997 • 280 citations

Identification of Non-Heme Diiron Proteins That Catalyze Triple Bond and Epoxy Group Formation

1998 • 261 citations

Crystal structure of human glyoxalase I_evidence for gene duplication and 3D domain swapping

1997 • 232 citations

How Big Is the Universe of Exons?

1990 • 228 citations

Three-dimensional Structures of Free Form and Two Substrate Complexes of an Extradiol Ring-cleavage Type Dioxygenase, the BphC Enzyme fromPseudomonassp. Strain KKS102

1996 • 209 citations

Three-dimensional structure of the binuclear metal center of phosphotriesterase

1995 • 200 citations

Metabolism of Ferulic Acid to Vanillin

1998 • 199 citations

Crystal structure of enoyl-coenzyme A (CoA) hydratase at 2.5 angstroms resolution: a spiral fold defines the CoA-binding pocket.

1996 • 189 citations

AtzC Is a New Member of the Amidohydrolase Protein Superfamily and Is Homologous to Other Atrazine-Metabolizing Enzymes

1998 • 174 citations

Structure of 4-Chlorobenzoyl Coenzyme A Dehalogenase Determined to 1.8 Å Resolution: An Enzyme Catalyst Generated via Adaptive Mutation^,

1996 • 155 citations

Fosfomycin Resistance Protein (FosA) Is a Manganese Metalloglutathione Transferase Related to Glyoxalase I and the Extradiol Dioxygenases

1997 • 141 citations

A Functionally Diverse Enzyme Superfamily that Abstracts the α Protons of Carboxylic Acids

1995 • 138 citations

Mandelate racemase and muconate lactonizing enzyme are mechanistically distinct and structurally homologous

1990 • 128 citations

A pre-transition-state mimic of an enzyme: x-ray structure of adenosine deaminase with bound 1-deazaadenosine and zinc-activated water

1993 • 121 citations

Molecular characterization of the cai operon necessary for carnitine metabolism in Escherichia coii

1994 • 116 citations

The crystal structure of enoyl-CoA hydratase complexed with octanoyl-CoA reveals the structural adaptations required for binding of a long chain fatty acid-CoA molecule 1 1Edited by D. Rees

1998 • 115 citations

Metal-Catalyzed Oxidation and Mutagenesis Studies on the Iron(II) Binding Site of 1-Aminocyclopropane-1-carboxylate Oxidase

1997 • 91 citations

Insights into the Mechanism of Catalysis by the P−C Bond-Cleaving Enzyme Phosphonoacetaldehyde Hydrolase Derived from Gene Sequence Analysis and Mutagenesis

1998 • 74 citations

Enoyl-CoA Hydratase and Isomerase form a Superfamily with a Common Active-Site Glutamate Residue

1995 • 72 citations

Enoyl-CoA Hydratase and Isomerase form a Superfamily with a Common Active-Site Glutamate Residue

1995 • 67 citations

Biochemical Characterization and Crystallographic Structure of an Escherichia coli Protein from the Phosphotriesterase Gene Family

1998 • 63 citations

The B ₁₂ -dependent ribonucleotide reductase from the archaebacterium Thermoplasma acidophila : An evolutionary solution to the ribonucleotide reductase conundrum

1997 • 63 citations

Octahedral coordination at the high-affinity metal site in enolase: Crystallographic analysis of the MgII-enzyme complex from yeast at 1.9 .ANG. resolution

1995 • 51 citations

Evolution in action

1995 • 49 citations

Primary Structure and Tissue-specific Expression of Human β-Hydroxyisobutyryl-coenzyme A Hydrolase

1996 • 48 citations

Epimerization via Carbon−Carbon Bond Cleavage. <scp>l</scp>-Ribulose-5-phosphate 4-Epimerase as a Masked Class II Aldolase

1998 • 41 citations

Enoyl-Coenzyme A Hydratase-Catalyzed Exchange of the .alpha.-Protons of Coenzyme A Thiol Esters: A Model for an Enolized Intermediate in the Enzyme-Catalyzed Elimination?

1994 • 38 citations

Isomerization of (R)- and (S)- glutathiolactaldehydes by glyoxalase I: the case for dichotomous stereochemical behavior in a single active site

1992 • 36 citations

Identification of Active Site Residues Essential to 4-Chlorobenzoyl−Coenzyme A Dehalogenase Catalysis by Chemical Modification and Site Directed Mutagenesis

1996 • 29 citations

Evolution of Enzymatic Activities in the Enolase Superfamily: Crystal Structure of (D)-Glucarate Dehydratase from Pseudomonas putida^,

1998 • 25 citations

Evolution of Enzymatic Activities: Multiple Pathways for Generating and Partitioning a Common Enolic Intermediate by Glucarate Dehydratase from Pseudomonas putida

1996 • 21 citations

Enantioselective Synthesis of the Macrolide Antibiotic Oleandomycin Aglycon

1996 • 17 citations

Evolution of Enzymatic Activities in the Enolase Superfamily: Partitioning of Reactive Intermediates by (D)-Glucarate Dehydratase from Pseudomonas putida

1998 • 16 citations