The Enolase Superfamily: A General Strategy for Enzyme-Catalyzed Abstraction of the α-Protons of Carboxylic Acids

Basic local alignment search tool

1990 • 87,358 citations

Improved tools for biological sequence comparison.

1988 • 11,455 citations

Whole-Genome Random Sequencing and Assembly of Haemophilus influenzae Rd

1995 • 5,571 citations

The MIDAS display system

1988 • 955 citations

On the Evolution of Biochemical Syntheses

1945 • 595 citations

The evolution of α/β barrel enzymes

1990 • 378 citations

An explanation for rapid enzyme-catalyzed proton abstraction from carbon acids: importance of late transition states in concerted mechanisms

1993 • 370 citations

Understanding the rates of certain enzyme-catalyzed reactions: Proton abstraction from carbon acids, acyl transfer reactions, and displacement reactions of phosphodiesters

1993 • 352 citations

Sequence Analysis of the Genome of the Unicellular Cyanobacterium Synechocystis sp. strain PCC6803. I. Sequence Features in the 1 Mb Region from Map Positions 64% to 92% of the Genome

1995 • 301 citations

Sensing Starvation: a Homoserine Lactone—Dependent Signaling Pathway in Escherichia coli

1994 • 215 citations

The primary structures of two yeast enolase genes. Homology between the 5' noncoding flanking regions of yeast enolase and glyceraldehyde-3-phosphate dehydrogenase genes.

1981 • 215 citations

DNA Sequence and Analysis of 136 Kilobases of the Escherichia coli Genome: Organizational Symmetry around the Origin of Replication

1993 • 215 citations

Relation between structure and function of α/β–protejns

1980 • 194 citations

Understanding enzyme-catalyzed proton abstraction from carbon acids: details of stepwise mechanisms for .beta.-elimination reactions

1992 • 177 citations

Sequence analysis of the Pseudomonas sp. strain P51 tcb gene cluster, which encodes metabolism of chlorinated catechols: evidence for specialization of catechol 1,2-dioxygenases for chlorinated substrates

1991 • 170 citations

Mandelate pathway of Pseudomonas putida: sequence relationships involving mandelate racemase, (S)-mandelate dehydrogenase, and benzoylformate decarboxylase and expression of benzoylformate decarboxylase in Escherichia coli

1990 • 156 citations

A Functionally Diverse Enzyme Superfamily that Abstracts the α Protons of Carboxylic Acids

1995 • 138 citations

Mandelate racemase and muconate lactonizing enzyme are mechanistically distinct and structurally homologous

1990 • 128 citations

A Carboxylate Oxygen of the Substrate Bridges the Magnesium Ions at the Active Site of Enolase: Structure of the Yeast Enzyme Complexed with the Equilibrium Mixture of 2-Phosphoglycerate and Phosphoenolpyruvate at 1.8 Å Resolution^,

1996 • 128 citations

On the origin of enzymatic species

1993 • 124 citations

Cloning and characterization of a human c-myc promoter-binding protein.

1991 • 118 citations

The role of lysine 166 in the mechanism of mandelate racemase from Pseudomonas putida: Mechanistic and crystallographic evidence for stereospecific alkylation by (R)-.alpha.-phenylglycidate

1994 • 108 citations

Mechanism of the reaction catalyzed by mandelate racemase: structure and mechanistic properties of the D270N mutant.

1996 • 101 citations

Crystal structure of enolase indicates that enolase and pyruvate kinase evolved from a common ancestor

1988 • 97 citations

Chelation of Serine 39 to Mg2+ Latches a Gate at the Active Site of Enolase: Structure of the Bis(Mg2+) Complex of Yeast Enolase and the Intermediate Analog Phosphonoacetohydroxamate at 2.1-.ANG. Resolution

1994 • 95 citations

Magnesium ion requirements for yeast enolase activity

1977 • 92 citations

Mandelate Racemase: Structure-Function Studies of a Pseudosymmetric Enzyme

1995 • 88 citations

Discontinuities in the evolution of Pseudomonas putida cat genes

1995 • 84 citations

Isolation and properties of the glycolytic enzymes from Zymomonas mobilis. The five enzymes from glyceraldehyde-3-phosphate dehydrogenase through to pyruvate kinase

1986 • 75 citations

Mechanism of the Reaction Catalyzed by Mandelate Racemase: Importance of Electrophilic Catalysis by Glutamic Acid 317

1995 • 70 citations

Mechanism of the reaction catalyzed by mandelate racemase. 3. Asymmetry in reactions catalyzed by the H297N mutant

1991 • 67 citations

Toward Identification of Acid/Base Catalysts in the Active Site of Enolase: Comparison of the Properties of K345A, E168Q, and E211Q Variants

1996 • 67 citations

3-Carboxy-cis,cis-muconate lactonizing enzyme from Pseudomonas putida is homologous to the class II fumarase family: a new reaction in the evolution of a mechanistic motif

1992 • 67 citations

Mechanism of the Reaction Catalyzed by Mandelate Racemase: Structure and Mechanistic Properties of the K166R Mutant

1995 • 63 citations

The Refined X-ray Structure of Muconate Lactonizing Enzyme fromPseudomonas putidaPRS2000 at 1.85 Å Resolution

1995 • 61 citations

Octameric enolase from the hyperthermophilic bacterium Thermotoga maritima: Purification, characterization, and image processing

1995 • 54 citations

Menaquinone (vitamin K2) biosynthesis: cloning, nucleotide sequence, and expression of the menC gene from Escherichia coli

1993 • 52 citations

Cloning and characterization of a naturally occurring antisense RNA to human thymidylate synthase mRNA

1993 • 51 citations

Octahedral coordination at the high-affinity metal site in enolase: Crystallographic analysis of the MgII-enzyme complex from yeast at 1.9 .ANG. resolution

1995 • 51 citations

Operon structure and nucleotide homology of the chlorocatechol oxidation genes of plasmids pJP4 and pAC27

1989 • 50 citations

Cloning, sequencing, and expression in Escherichia coli of the Clostridium tetanomorphum gene encoding .beta.-methylaspartase and characterization of the recombinant protein

1992 • 45 citations

Unusual G + C content and codon usage in catIJF, a segment of the bencat supra-operonic cluster in the Acinetobacter calcoaceticus chromosome

1994 • 43 citations

Structure of the bis divalent cation complex with phosphonoacetohydroxamate at the active site of enolase

1992 • 39 citations

Purification and properties of thermostable N-acylamino acid racemase from Amycolatopsis sp. TS-1-60

1995 • 38 citations

Characterization of the interaction of yeast enolase with polynucleotides

1992 • 37 citations

Absolute stereochemical course of the 3-carboxymuconate cycloisomerases from Pseudomonas putida and Acinetobacter calcoaceticus: analysis and implications

1987 • 36 citations

Biosynthesis of o-succinylbenzoic acid II: Properties of o-succinylbenzoic acid synthase, an enzyme involved in vitamin K2 biosynthesis

1987 • 31 citations

Cloning, DNA sequence analysis, and expression in Escherichia coli of the gene for mandelate racemase from Pseudomonas putida

1988 • 30 citations

Halobacterial S9 operon. Three ribosomal protein genes are cotranscribed with genes encoding a tRNA(Leu), the enolase, and a putative membrane protein in the archaebacterium Haloarcula (Halobacterium) marismortui.

1991 • 29 citations

The metabolism of <scp>d</scp>-glucarate by Pseudomonas acidovorans

1969 • 23 citations

Evolution of Enzymatic Activities: Multiple Pathways for Generating and Partitioning a Common Enolic Intermediate by Glucarate Dehydratase from Pseudomonas putida

1996 • 21 citations

7 Stereochemistry of Enzyme -Catalyzed Reactions at Carbon

1990 • 21 citations

Cloning, DNA sequencing and heterologous expression of the gene for thermostable N-acylamino acid racemase from Amycolatopsis sp. TS-1-60 in Escherichia coli

1995 • 21 citations

Cloning, DNA sequencing and heterologous expression of the gene for thermostable N -acylamino acid racemase from Amycolatopsis sp. TS-1-60 in Escherichia coli

1995 • 20 citations

The carboxyphosphonoenolpyruvate synthase-encoding gene from the bialaphos-producing organism Streptomyces hygroscopicus

1995 • 19 citations

Analysis of genome instability in Streptomyces ambofaciens

1993 • 18 citations

Enolase from spores and cells of Bacillus megaterium: two-step purification of the enzyme and some of its properties

1978 • 17 citations

Determination of a 21548 bp nucleotide sequence around the 24 region of the Bacillus subtilis chromosome

1995 • 13 citations

[118] d-glucarate dehydrase

1966 • 12 citations