Identification of Active Site Residues Essential to 4-Chlorobenzoyl−Coenzyme A Dehalogenase Catalysis by Chemical Modification and Site Directed Mutagenesis

A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding

1976 • 225,752 citations

A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding

1976 • 200,127 citations

DNA sequencing with chain-terminating inhibitors

1977 • 69,181 citations

The α/β hydrolase fold

1992 • 2,024 citations

[41] Modification of histidyl residues in proteins by diethylpyrocarbonate

1977 • 813 citations

PCR Technology: Principles and Applications for DNA Amplification

1990 • 512 citations

Crystallographic analysis of the catalytic mechanism of haloalkane dehalogenase

1993 • 463 citations

Ethoxyformylation of proteins. Reaction of ethoxyformic anhydride with .alpha.-chymotrypsin, pepsin, and pancreatic ribonuclease at pH 4

1970 • 388 citations

Ancestry of the 4-chlorobenzoate dehalogenase: analysis of amino acid sequence identities among families of acyl:adenyl ligases, enoyl-CoA hydratases/isomerases, and acyl-CoA thioesterases

1992 • 194 citations

Novel Enzymic Hydrolytic Dehalogenation of a Chlorinated Aromatic

1991 • 168 citations

The catalytic mechanism of microsomal epoxide hydrolase involves an ester intermediate

1993 • 158 citations

Structure of 4-Chlorobenzoyl Coenzyme A Dehalogenase Determined to 1.8 Å Resolution: An Enzyme Catalyst Generated via Adaptive Mutation^,

1996 • 155 citations

Refined X-ray Structures of Haloalkane Dehalogenase at pH 6·2 and pH 8·2 and Implications for the Reaction Mechanism

1993 • 117 citations

Bacterial dehalogenation of chlorobenzoates and coculture biodegradation of 4,4'-dichlorobiphenyl

1989 • 106 citations

Evaluation of 5-enolpyruvoylshikimate-3-phosphate synthase substrate and inhibitor binding by stopped-flow and equilibrium fluorescence measurements

1988 • 103 citations

Site-Directed Mutagenesis and Oxygen Isotope Incorporation Studies of the Nucleophilic Aspartate of Haloalkane Dehalogenase

1994 • 93 citations

Isolation and characterization of the three polypeptide components of 4-chlorobenzoate dehalogenase from Pseudomonas sp. strain CBS-3

1992 • 92 citations

Cloning and sequence analysis of genes for dehalogenation of 4-chlorobenzoate from Arthrobacter sp. strain SU

1992 • 78 citations

Crystallographic and fluorescence studies of the interaction of haloalkane dehalogenase with halide ions. Studies with halide compounds reveal a halide binding site in the active site

1993 • 73 citations

On the origins and functions of the enzymes of the 4-chlorobenzoate to 4-hydroxybenzoate converting pathway

1994 • 64 citations

Microsomal and Soluble Epoxide Hydrolases Are Members of the Same Family of C-X Bond Hydrolase Enzymes

1994 • 57 citations

The action of N-bromosuccinimide on chymotrypsin

1961 • 45 citations

Evidence for Nucleophilic Catalysis in the Aromatic Substitution Reaction Catalyzed by (4-Chlorobenzoyl)coenzyme A Dehalogenase

1994 • 42 citations

Exploration of possible mechanisms for 4-chlorobenzoyl CoA dehalogenase: Evidence for an aryl-enzyme intermediate

1995 • 35 citations

[5] Computer-assisted derivation of steady-state rate equations

1979 • 34 citations

Evidence for electrophilic catalysis in the 4-chlorobenzoyl-CoA dehalogenase reaction: UV, Raman, and 13C-NMR spectral studies of dehalogenase complexes of benzoyl-CoA adducts

1995 • 33 citations

Enzymic Dehalogenation of 4-Chlorobenzoyl Coenzyme A in Acinetobacter sp. Strain 4-CB1

1992 • 30 citations

Inactivation of rat liver S-adenosylhomocysteinase by iodoacetamide

1982 • 30 citations

Anaerobic bioformation of 4-hydroxybenzoate from 4-chlorobenzoate by the coryneform bacterium NTB-1

1992 • 30 citations

Specificity of 4-chlorobenzoyl coenzyme A dehalogenase catalyzed dehalogenation of halogenated aromatics

1993 • 28 citations

Transient State Kinetic Analysis of the Chemical Intermediates Formed in the Enzymic Dehalogenation of 4-Chlorobenzoyl Coenzyme A

1995 • 22 citations

Dehalogenation of 4-chlorobenzoate

1995 • 21 citations