Evolutionary Divergence of Substrate Specificity within the Chymotrypsin-like Serine Protease Fold

The refined 1.9 A crystal structure of human alpha-thrombin: interaction with D-Phe-Pro-Arg chloromethylketone and significance of the Tyr-Pro-Pro-Trp insertion segment.

1989 • 889 citations

Structural basis of substrate specificity in the serine proteases

1995 • 802 citations

Converting Trypsin to Chymotrypsin: The Role of Surface Loops

1992 • 461 citations

Redesigning Trypsin: Alteration of Substrate Specificity

1985 • 357 citations

Understanding Enzyme Superfamilies

1997 • 280 citations

A Specific, Highly Active Malate Dehydrogenase by Redesign of a Lactate Dehydrogenase Framework

1988 • 276 citations

Crystallographic and NMR Studies of the Serine Proteases

1982 • 199 citations

Structural plasticity broadens the specificity of an engineered protease

1989 • 165 citations

Substrate specificity of trypsin investigated by using a genetic selection.

1990 • 160 citations

Selective alteration of substrate specificity by replacement of aspartic acid-189 with lysine in the binding pocket of trypsin

1987 • 158 citations

A new serine-protease fold revealed by the crystal structure of human cytomegalovirus protease

1996 • 156 citations

Structure of the Human Cytomegalovirus Protease Catalytic Domain Reveals a Novel Serine Protease Fold and Catalytic Triad

1996 • 150 citations

Amino acid residues that affect interaction of tissue-type plasminogen activator with plasminogen activator inhibitor 1.

1990 • 148 citations

Unique fold and active site in cytomegalovirus protease

1996 • 147 citations

Three-dimensional structure of human cytomegalovirus protease

1996 • 146 citations

The crystal structure of the catalytic domain of human urokinase-type plasminogen activator

1995 • 145 citations

Structural origins of substrate discrimination in trypsin and chymotrypsin

1995 • 137 citations

Serine protease mechanism: structure of an inhibitory complex of .alpha.-lytic protease and a tightly bound peptide boronic acid

1987 • 137 citations

Converting Trypsin to Chymotrypsin: Residue 172 Is a Substrate Specificity Determinant

1994 • 134 citations

Cloning and functional expression of a cDNA encoding the catalytic subunit of bovine enterokinase.

1993 • 114 citations

A highly active decarboxylating dehydrogenase with rationally inverted coenzyme specificity.

1995 • 108 citations

Structure of wheat serine carboxypeptidase II at 3.5-A resolution. A new class of serine proteinase.

1990 • 105 citations

Structure of wheat serine carboxypeptidase II at 3.5-A resolution. A new class of serine proteinase.

1990 • 87 citations

Structure of Human Factor D

1994 • 83 citations

Crystal Structure of an Ecotin−Collagenase Complex Suggests a Model for Recognition and Cleavage of the Collagen Triple Helix

1997 • 69 citations

Relocating a Negative Charge in the Binding Pocket of Trypsin

1993 • 68 citations

Converting Trypsin to Chymotrypsin: Ground-State Binding Does Not Determine Substrate Specificity

1994 • 67 citations

The substrate specificity of Uca pugilator collagenolytic serine protease 1 correlates with the bovine type I collagen cleavage sites.

1994 • 45 citations

Exogenous Acetate Reconstitutes the Enzymic Activity of Trypsin Asp189Ser

1994 • 44 citations

Stepwise improvements in catalytic effectiveness: independence and interdependence in combinations of point mutations of a sluggish triosephosphate isomerase

1991 • 43 citations

Structural Basis for the Broad Substrate Specificity of Fiddler Crab Collagenolytic Serine Protease 1

1997 • 42 citations

Engineered Metal Regulation of Trypsin Specificity

1995 • 38 citations

Functional Linkage Between the Active Site of α-Lytic Protease and Distant Regions of Structure: Scanning Alanine Mutagenesis of a Surface Loop Affects Activity and Substrate Specificity

1995 • 37 citations

Kinetic and Structural Characterization of Mutations of Glycine 216 in α-Lytic Protease: A New Target for Engineering Substrate Specificity

1995 • 34 citations

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