Deoxynucleoside Triphosphate and Pyrophosphate Binding Sites in the Catalytically Competent Ternary Complex for the Polymerase Reaction Catalyzed by DNA Polymerase I (Klenow Fragment)

A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding

1976 • 225,752 citations

A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding

1976 • 200,127 citations

Crystal Structure at 3.5 Å Resolution of HIV-1 Reverse Transcriptase Complexed with an Inhibitor

1992 • 1,887 citations

Structural basis for the 3′-5′ exonuclease activity of Escherichia coli DNA polymerase I: a two metal ion mechanism.

1991 • 1,059 citations

Structure of large fragment of Escherichia coli DNA polymerase I complexed with dTMP

1985 • 855 citations

Structures of ternary complexes of rat DNA polymerase beta, a DNA template-primer, and ddCTP.

1994 • 694 citations

An attempt to unify the structure of polymerases

1990 • 640 citations

FUNCTION AND STRUCTURE RELATIONSHIPS IN DNA POLYMERASES

1994 • 611 citations

Compilation, alignment, and phylogenetic relationships of DNA polymerases

1993 • 590 citations

Structures of Ternary Complexes of Rat DNA Polymerase β, a DNA Template-Primer, and ddCTP

1994 • 587 citations

Structure of DNA Polymerase I Klenow Fragment Bound to Duplex DNA

1993 • 473 citations

Crystal Structure of Rat DNA Polymerase β: Evidence for a Common Polymerase Mechanism

1994 • 464 citations

Crystal structure of bacteriophage T7 RNA polymerase at 3.3 Å resolution

1993 • 358 citations

Kinetic mechanism of DNA polymerase I (Klenow)

1987 • 352 citations

Genetic and Crystallographic Studies of the 3′,5′-Exonucleolytic Site of DNA Polymerase I

1988 • 344 citations

Cocrystal structure of an editing complex of Klenow fragment with DNA.

1988 • 324 citations

The 3′-5′ exonuclease of DNA polymerase I of Escherichia coli: contribution of each amino acid at the active site to the reaction.

1991 • 279 citations

Identification of residues critical for the polymerase activity of the Klenow fragment of DNA polymerase I from Escherichia coli.

1990 • 261 citations

Thermodynamic parameters for loop formation in RNA and DNA hairpin tetraloops

1992 • 241 citations

DNA sequence, structure and gene expression of mycobacteriophage L5: a phage system for mycobacterial genetics

1993 • 223 citations

DNA- and RNA-dependent DNA polymerases

1993 • 218 citations

Kinetic mechanism of DNA polymerase I(Klenow fragment): identification of a second conformational change and evaluation of the internal equilibrium constant

1991 • 218 citations

Side chains involved in catalysis of the polymerase reaction of DNA polymerase I from Escherichia coli.

1992 • 194 citations

Elementary steps in the DNA polymerase I reaction pathway

1983 • 179 citations

Crystal structures of the Klenow fragment of DNA polymerase I complexed with deoxynucleoside triphosphate and pyrophosphate

1993 • 175 citations

A study of the mechanism of DNA polymerase I from Escherichia coli with diastereomeric phosphorothioate analogs of deoxyadenosine triphosphate.

1979 • 172 citations

2.3 Å crystal structure of the catalytic domain of DNA polymerase β

1994 • 168 citations

Substrate Activation of Trypsin^*

1963 • 157 citations

A mutant of DNA polymerase I (Klenow fragment) with reduced fidelity

1991 • 124 citations

The steady state kinetic parameters and non-processivity of Escherichia coli deoxyribonucleic acid polymerase I

1975 • 120 citations

DNA polymerase .epsilon.: Aphidicolin inhibition and the relationship between polymerase and exonuclease activity

1993 • 100 citations

A domain of the klenow fragment of Escherichia coli DNA polymerase I has polymerase but no exonuclease activity

1986 • 90 citations

Mechanism of DNA replication fidelity for three mutants of DNA polymerase I: Klenow fragment KF(exo+), KF(polA5), and KF(exo-)

1991 • 81 citations

The minimal kinetic mechanism for misincorporation by DNA polymerase I (Klenow fragment)

1992 • 76 citations

Mutational studies of human DNA polymerase alpha. Identification of residues critical for deoxynucleotide binding and misinsertion fidelity of DNA synthesis.

1993 • 73 citations

Identification and amino acid sequence of the deoxynucleoside triphosphate binding site in Escherichia coli DNA polymerase I

1987 • 73 citations

Identification, sequencing, and targeted mutagenesis of a DNA polymerase gene required for the extreme radioresistance of Deinococcus radiodurans

1993 • 67 citations

Photoaffinity labeling of the thymidine triphosphate binding domain in Escherichia coli DNA polymerase I: identification of histidine-881 as the site of cross-linking

1987 • 59 citations

Photoaffinity labeling of the Klenow fragment with 8-azido-dATP.

1990 • 58 citations

Phi 29 DNA polymerase active site. Mutants in conserved residues Tyr254 and Tyr390 are affected in dNTP binding.

1992 • 58 citations

Cloning of the DNA Polymerase Gene of Bacillus caldotenax and Characterization of the Gene Product1

1993 • 54 citations

Phi 29 DNA polymerase active site. The conserved amino acid motif “Kx3NSxYG” is involved in template-primer binding and dNTP selection

1993 • 54 citations

Sequence of bacteriophage T3 DNA from gene 2.5 through gene 9

1989 • 41 citations

Hydrolysis of a stable oxygen ester of phosphorothioic acid by alkaline phosphatase

1972 • 34 citations

Role of lysine 758 of Escherichia coli DNA polymerase I as assessed by site-directed mutagenesis.

1994 • 22 citations

Cloning, nucleotide sequence, and expression in escherichia coli of DNA polymerase gene (polA) from thermus thermophilus HB8

1993 • 17 citations

Site directed mutagenesis of DNA polymerase I (Klenow) from Escherichia coli

1993 • 15 citations

Template primer-dependent binding of 5'-fluorosulfonyl-benzoyldeoxyadenosine by Escherichia coli DNA polymerase I. Identification of arginine 682 as the binding site and its implication in catalysis.

1990 • 14 citations