Isolation, Characterization, and Expression in Escherichia coli of the DNA Polymerase Gene from Thermus aquaticus

Molecular Cloning: A Laboratory Manual

2001 • 133,517 citations

Experiments in molecular genetics

1972 • 28,260 citations

Molecular Cloning. A Laboratory Manual

1983 • 27,328 citations

Primer-Directed Enzymatic Amplification of DNA with a Thermostable DNA Polymerase

1988 • 17,059 citations

A rapid alkaline extraction procedure for screening recombinant plasmid DNA

1979 • 15,511 citations

Studies on transformation of Escherichia coli with plasmids

1983 • 11,179 citations

Enzymatic Amplification of β-Globin Genomic Sequences and Restriction Site Analysis for Diagnosis of Sickle Cell Anemia

1985 • 9,128 citations

[21] Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction

1987 • 5,745 citations

[2] New M13 vectors for cloning

1983 • 5,402 citations

Analysis of gene control signals by DNA fusion and cloning in Escherichia coli

1980 • 2,499 citations

Efficient isolation of genes by using antibody probes.

1983 • 2,065 citations

Yeast RNA Polymerase II Genes: Isolation with Antibody Probes

1983 • 1,084 citations

Structure of large fragment of Escherichia coli DNA polymerase I complexed with dTMP

1985 • 855 citations

An interactive graphics program for comparing and aligning nucleic acid and amino acid sequences

1982 • 801 citations

DNA sequencing with Thermus aquaticus DNA polymerase and direct sequencing of polymerase chain reaction-amplified DNA.

1988 • 775 citations

Improvement of the dideoxy chain termination method of DNA sequencing by use of deoxy-7-deazaguanosine triphosphate in place of dGTP

1986 • 717 citations

Host specificity of DNA produced by Escherichia coli: Bacterial mutations affecting the restriction and modification of DNA

1966 • 706 citations

Deoxyribonucleic acid polymerase from the extreme thermophile Thermus aquaticus

1976 • 662 citations

Determination of Nucleotide Sequences in DNA

1981 • 593 citations

Thermal stability and protein structure

1979 • 518 citations

Sequence diversity among related genes for recognition of specific targets in DNA molecules

1983 • 478 citations

mRNA polyadenylate-binding protein: gene isolation and sequencing and identification of a ribonucleoprotein consensus sequence.

1986 • 455 citations

Thermophilic blue-green algae and the thermal environment

1969 • 447 citations

Escherichia coli thioredoxin confers processivity on the DNA polymerase activity of the gene 5 protein of bacteriophage T7.

1987 • 399 citations

An improved filamentous helper phage for generating single-stranded plasmid DNA

1986 • 348 citations

Genetic and Crystallographic Studies of the 3′,5′-Exonucleolytic Site of DNA Polymerase I

1988 • 344 citations

McrA and McrB restriction phenotypes of someE.colistrains and implications for gene cloning

1988 • 337 citations

EcoRI endonuclease. Physical and catalytic properties of the homogenous enzyme.

1976 • 259 citations

High guanine plus cytosine content in the third letter of codons of an extreme thermophile. DNA sequence of the isopropylmalate dehydrogenase of Thermus thermophilus.

1984 • 207 citations

A simple, general procedure for purifying restriction endonucleases

1977 • 192 citations

Lambda Charon vectors (Ch32, 33, 34 and 35) adapted for DNA cloning in recombination-deficient hosts

1983 • 191 citations

An active fragment of DNA polymerase produced by proteolytic cleavage

1969 • 190 citations

[5] Kilo-sequencing: Creation of an ordered nest of asymmetric deletions across a large target sequence carried on phage M13

1983 • 169 citations

DNA polymerase I gene of Saccharomyces cerevisiae: nucleotide sequence, mapping of a temperature-sensitive mutation, and protein homology with other DNA polymerases.

1988 • 168 citations

Proteins from thermophilic microorganisms

1973 • 158 citations

Structural and functional relationships between prokaryotic and eukaryotic DNA polymerases.

1987 • 157 citations

Insertion mutations in the dam gene of Escherichia coli K-12

1983 • 138 citations

Domain of E. coli DNA polymerase I showing sequence homology to T7 DNA polymerase

1985 • 118 citations

Nucleotide sequence of the malate dehydrogenase gene of Thermus flavus and its mutation directing an increase in enzyme activity.

1986 • 100 citations

A domain of the klenow fragment of Escherichia coli DNA polymerase I has polymerase but no exonuclease activity

1986 • 90 citations

Cloning, sequencing and expression of theTaqI restriction-modification system

1987 • 89 citations

A microtubule-associated protein in Drosophila melanogaster: identification, characterization, and isolation of coding sequences.

1986 • 87 citations

Identification and amino acid sequence of the deoxynucleoside triphosphate binding site in Escherichia coli DNA polymerase I

1987 • 73 citations

Genetic mapping and DNA sequence analysis of mutations in the polA gene of Escherichia coli

1985 • 68 citations

Cloning and expression of the leucine gene from Thermus thermophilus in Escherichia coli

1980 • 64 citations

Use of nonisotopic M13 probes for genetic analysis: application to HLA class II loci.

1986 • 59 citations

Photoaffinity labeling of the thymidine triphosphate binding domain in Escherichia coli DNA polymerase I: identification of histidine-881 as the site of cross-linking

1987 • 59 citations

Nucleotide sequence and characteristics of the gene for <scp>l</scp>‐lactate dehydrogenase of Thermus caldophilus GK24 and the deduced amino‐acid sequence of the enzyme

1986 • 58 citations

Isolation and characterization of a lambdapolA transducing phage.

1977 • 57 citations

Selective oxidation of the exonuclease domain of bacteriophage T7 DNA polymerase.

1987 • 56 citations

Amino acid composition and thermal stability of proteins

1982 • 51 citations

A new specific endonuclease present in Xanthomonas holcicola, Xanthomonas papavericola and Brevibacterium luteum

1978 • 49 citations

Cloning and expression of T4 DNA polymerase.

1987 • 42 citations

Detection of activated Mr 21,000 protein, the product of ras oncogenes, using antibodies with specificity for amino acid 12.

1986 • 39 citations

Regulated expression by readthrough translation from a plasmid-encoded beta-galactosidase

1978 • 37 citations

Nucleotide sequence ofThermus thermophilusHB8 gene coding 16S rRNA

1988 • 37 citations

DNA binding domain of Escherichia coli DNA polymerase I: identification of arginine-841 as an essential residue

1988 • 33 citations

Affinity labeling of Escherichia coli DNA polymerase I by 5'-fluorosulfonylbenzoyladenosine. Identification of the domain essential for polymerization and Arg-682 as the site of reactivity.

1988 • 32 citations

Hepatitis B virus particles contain a polypeptide encoded by the largest open reading frame: a putative reverse transcriptase

1988 • 32 citations

The purification of restriction endonuclease EcoRI by precipitation involving polyethyleneimine

1977 • 26 citations

Ferrate oxidation of Escherichia coli DNA polymerase-I. Identification of a methionine residue that is essential for DNA binding.

1987 • 26 citations

polA6, a mutation affecting the DNA binding capacity of DNA polymerase I

1976 • 23 citations

On the processive mechanism of Escherichia coli DNA Polymerase I. The polA5 mutation

1978 • 22 citations

Purification and electrophoretic assay of T4-induced polynucleotide ligase for the in vitro construction of recombinant DNA molecules

1976 • 11 citations