Interleukin-12 as an Adjuvant for Cancer Immunotherapy

Interleukin-12: A Proinflammatory Cytokine with Immunoregulatory Functions that Bridge Innate Resistance and Antigen-Specific Adaptive Immunity

1995 • 2,437 citations

Requirement for V _α 14 NKT Cells in IL-12-Mediated Rejection of Tumors

1997 • 1,226 citations

Regulation of the Interleukin (IL)-12R β2 Subunit Expression in Developing T Helper 1 (Th1) and Th2 Cells

1997 • 1,022 citations

Cloning of cDNA for natural killer cell stimulatory factor, a heterodimeric cytokine with multiple biologic effects on T and natural killer cells.

1991 • 861 citations

Interleukin 12 is required for the T-lymphocyte-independent induction of interferon gamma by an intracellular parasite and induces resistance in T-cell-deficient hosts.

1993 • 837 citations

Recombinant IL-12 administration induces tumor regression in association with IFN-gamma production.

1994 • 796 citations

Effects of Single-Dose Interleukin-12 Exposure on Interleukin-12–Associated Toxicity and Interferon-γ Production

1997 • 788 citations

Selective Expression of an Interleukin-12 Receptor Component by Human T Helper 1 Cells

1997 • 783 citations

Inhibition of Angiogenesis In Vivo by Interleukin 12

1995 • 734 citations

The Adjuvant Effect of Interleukin-12 in a Vaccine Against Leishmania major

1994 • 687 citations

Interleukin 12 signaling in T helper type 1 (Th1) cells involves tyrosine phosphorylation of signal transducer and activator of transcription (Stat)3 and Stat4.

1995 • 675 citations

Phase I evaluation of intravenous recombinant human interleukin 12 in patients with advanced malignancies.

1997 • 556 citations

Inhibition of angiogenesis by interleukin-12 is mediated by the interferon-inducible protein 10

1996 • 480 citations

Interleukin 12 induces tyrosine phosphorylation and activation of STAT4 in human lymphocytes.

1995 • 446 citations

Interleukin-12 and interleukin-18 synergistically induce murine tumor regression which involves inhibition of angiogenesis.

1998 • 419 citations

An IL-12-based vaccination method for preventing fibrosis induced by schistosome infection

1995 • 403 citations

Restoration of HIV-Specific Cell-Mediated Immune Responses by Interleukin-12 in Vitro

1993 • 397 citations

Clinical Trials: IL-12 Deaths: Explanation and a Puzzle

1995 • 333 citations

Interleukin‐12 profoundly up‐regulates the synthesis of antigen‐specific complement‐fixing IgG2a, IgG2b and IgG3 antibody subclasses in vivo

1995 • 331 citations

Tumor Cell Responses to IFNγ Affect Tumorigenicity and Response to IL-12 Therapy and Antiangiogenesis

1998 • 324 citations

IL-12-induced protection against blood-stage Plasmodium chabaudi AS requires IFN-gamma and TNF-alpha and occurs via a nitric oxide-dependent mechanism.

1995 • 314 citations

Interleukin 12–mediated Prevention of Spontaneous Mammary Adenocarcinomas in Two Lines of Her-2/neu Transgenic Mice

1998 • 299 citations

The CXC Chemokines IP-10 and Mig Are Necessary for IL-12-Mediated Regression of the Mouse RENCA Tumor

1998 • 295 citations

Effects of IL-12 on in vivo cytokine gene expression and Ig isotype selection.

1994 • 290 citations

Proinflammatory and Immunoregulatory Functions of Interleukin-12

1998 • 283 citations

Effective eradication of established murine tumors with IL-12 gene therapy using a polycistronic retroviral vector.

1995 • 277 citations

The role of IL-12 in the induction of organ-specific autoimmune diseases

1995 • 277 citations

Mig, the Monokine Induced By Interferon-γ, Promotes Tumor Necrosis In Vivo

1997 • 251 citations

Effects of IL-12 on immune responses to microbial infections: a key mediator in regulating disease outcome

1995 • 249 citations

Gene gun-mediated skin transfection with interleukin 12 gene results in regression of established primary and metastatic murine tumors.

1996 • 248 citations

Cytotoxic NK1.1 Ag+ alpha beta T cells with intermediate TCR induced in the liver of mice by IL-12.

1995 • 222 citations

Influence of interleukin 12 on p53 peptide vaccination against established Meth A sarcoma.

1995 • 218 citations

Mechanism of interleukin 12-mediated toxicities during experimental viral infections: role of tumor necrosis factor and glucocorticoids.

1995 • 215 citations

Recombinant interleukin-12 suppresses the synthesis of immunoglobulin E by interleukin-4 stimulated human lymphocytes.

1992 • 209 citations

Cancer immunotherapy of established tumors with IL-12. Effective delivery by genetically engineered fibroblasts.

1995 • 206 citations

IL-12 induces T helper 1-directed antitumor response.

1997 • 189 citations

Critical role of NK1+ T cells in IL-12-induced immune responses in vivo.

1998 • 179 citations

Liver NK1.1+ CD4+ alpha beta T cells activated by IL-12 as a major effector in inhibition of experimental tumor metastasis.

1996 • 175 citations

Antitumor Efficacy of Adenocarcinoma Cells Engineered to Produce Interleukin 12 (IL-12) or Other Cytokines Compared With Exogenous IL-12

1997 • 174 citations

Role of Interferon-γ in Mediating the Antitumor Efficacy of Interleukin-12

1995 • 172 citations

Vaccination with IL-12 gene-modified autologous melanoma cells: preclinical results and a first clinical phase I study

1998 • 167 citations

Cytokine and chemokine expression in tumors of mice receiving systemic therapy with IL-12.

1996 • 166 citations

Administration of Interleukin 12 With Pulse Interleukin 2 and the Rapid and Complete Eradication of Murine Renal Carcinoma

1996 • 164 citations

Interleukin-12. Biologic activity, therapeutic utility, and role in disease.

1995 • 163 citations

Cytokine-in-adjuvant steering of the immune response phenotype to HIV-1 vaccine constructs: granulocyte-macrophage colony-stimulating factor and TNF-alpha synergize with IL-12 to enhance induction of cytotoxic T lymphocytes.

1997 • 161 citations

Pilot study of subcutaneous recombinant human interleukin 12 in metastatic melanoma.

1998 • 160 citations

Bioactive murine and human interleukin-12 fusion proteins which retain antitumor activity in vivo

1997 • 146 citations

Administration of recombinant interleukin 12 prevents outgrowth of tumor cells metastasizing spontaneously to lung and lymph nodes.

1995 • 138 citations

Cutting Edge: Critical Role of NK1+ T Cells in IL-12-Induced Immune Responses In Vivo

1998 • 136 citations

Interleukin‐12 and B7.1 co‐stimulation cooperate in the induction of effective antitumor immunity and therapy of established tumors

1996 • 130 citations

IL-12 is an effective adjuvant to recombinant vaccinia virus-based tumor vaccines: enhancement by simultaneous B7-1 expression.

1996 • 129 citations

A tumor-associated and self antigen peptide presented by dendritic cells may induce T cell anergy in vivo, but IL-12 can prevent or revert the anergic state.

1997 • 127 citations

CD4 T cells inhibit in vivo the CD8‐mediated immune response against murine colon carcinoma cells transduced with interleukin‐12 genes

1995 • 125 citations

Oral but Not Parenteral Interleukin (IL)-12 Redirects T Helper 2 (Th2)-type Responses to an Oral Vaccine Without Altering Mucosal IgA Responses

1997 • 124 citations

B7-1 and interleukin 12 synergistically induce effective antitumor immunity.

1995 • 118 citations

Cytokine Gene Therapy of Cancer Using Gene Gun Technology: Superior Antitumor Activity of Interleukin-12

1997 • 118 citations

Costimulation with B7-1, IL-6, and IL-12 is sufficient for primary generation of murine antitumor cytolytic T lymphocytes in vitro.

1995 • 115 citations

Endogenous IL-12 is necessary for rejection of P815 tumor variants in vivo.

1996 • 88 citations

IL‐12‐Engineered Dendritic Cells Serve as Effective Tumor Vaccine Adjuvants in Vivoa

1996 • 86 citations

Nonviable bacterial antigens administered with IL-12 generate antigen-specific T cell responses and protective immunity against Listeria monocytogenes.

1995 • 79 citations

Altered Interleukin-12 Responsiveness in Th1 and Th2 Cells Is Associated With the Differential Activation of STAT5 and STAT1

1998 • 78 citations

Interleukin 12 potentiates the curative effect of a vaccine based on interleukin 2-transduced tumor cells.

1996 • 77 citations

High cytokine production and effective antitumor activity of a recombinant vaccinia virus encoding murine interleukin 12.

1995 • 76 citations

Gene therapy with a single chain interleukin 12 fusion protein induces T cell-dependent protective immunity in a syngeneic model of murine neuroblastoma

1998 • 71 citations

Immunity against Yersinia enterocolitica by vaccination with Yersinia HSP60 immunostimulating complexes or Yersinia HSP60 plus interleukin-12

1996 • 71 citations

The effect of interleukin 12 desensitization on the antitumor efficacy of recombinant interleukin 12.

1997 • 69 citations

Immune Response Against Large Tumors Eradicated by Treatment with Cyclophosphamide and IL-12

1998 • 68 citations

Injection of complementary DNA encoding interleukin-12 inhibits tumor establishment at a distant site in a murine renal carcinoma model.

1996 • 68 citations

IL-12 stimulates an antiviral type 1 cytokine response but lacks adjuvant activity in IFN-gamma-receptor-deficient mice.

1995 • 65 citations

Characterization of a novel subset of CD8(+) T cells that expands in patients receiving interleukin-12.

1998 • 65 citations

Multiple roles of interferon-gamma in the mediation of interleukin 12-induced tumor regression.

1998 • 65 citations

Interleukin-12 Treatment during Immunization Elicits a T Helper Cell Type 1-like Immune Response in Mice Challenged with Respiratory Syncytial Virus and Improves Vaccine Immunogenicity

1995 • 65 citations

IL-12 Gene Therapy Using Direct Injection of Tumors with Genetically Engineered Autologous Fibroblasts. University of Pittsburgh, Pittsburgh, Pennsylvania

1995 • 65 citations

Interleukin 12 primes macrophages for nitric oxide production in vivo and restores depressed nitric oxide production by macrophages from tumor-bearing mice: implications for the antitumor activity of interleukin 12 and/or interleukin 2.

1996 • 64 citations

Immunotherapy of experimental metastases by vaccination with interleukin gene-transduced adenocarcinoma cells sharing tumor-associated antigens. Comparison between IL-12 and IL-2 gene-transduced tumor cell vaccines.

1996 • 63 citations

Construction and Biological Characterization of an Interleukin-12 Fusion Protein (Flexi-12): Delivery to Acute Myeloid Leukemic Blasts Using Adeno-Associated Virus

1997 • 63 citations

Interferon γ–independent Rejection of Interleukin 12–transduced Carcinoma Cells Requires CD4+ T Cells and Granulocyte/Macrophage Colony–stimulating Factor

1998 • 61 citations

Recombinant interleukin 12 enhances cellular immune responses to vaccination only after a period of suppression.

1998 • 61 citations

Regulation of local host-mediated anti-tumor mechanisms by cytokines: direct and indirect effects on leukocyte recruitment and angiogenesis.

1997 • 59 citations

IL-12-induced tumor regression correlates with in situ activity of IFN-γ produced by tumor-infiltrating cells and its secondary induction of anti-tumor pathways

1997 • 59 citations

Interleukin 12 gene therapy of MHC-negative murine melanoma metastases.

1998 • 56 citations

Interleukin-12: An Immunoregulatory Cytokine Produced by B Cells and Antigen-Presenting Cells

1997 • 48 citations

IgG2a induced by interleukin (IL) 12-producing tumor cell vaccines but not IgG1 induced by IL-4 vaccine is associated with the eradication of experimental metastases.

1998 • 44 citations

IL-12 and Mutant P53 Peptide-Pulsed Dendritic Cells for the Specific Immunotherapy of Cancer

1996 • 39 citations

Interleukin-12 and malaria

1995 • 38 citations

Administration of Systemic or Local Interleukin-2 Enhances the Anti-tumor Effects of Interleukin-12 Gene Therapy

1995 • 38 citations

Interleukin‐12 induces cytotoxic NK1⁺αβ T cells in the lungs of euthymic and athymic mice

1996 • 37 citations

Immunodominance of cytotoxic T lymphocyte epitopes co‐injected in vivo and modulation by interleukin‐12

1996 • 36 citations

Construction of Vectors Expressing Bioactive Heterodimeric and Single-Chain Murine Interleukin-12 for Gene Therapy

1998 • 35 citations

Antitumor and Antimetastatic Activity of lnterleukin-12

1996 • 35 citations

Interleukin‐12: Murine Models of a Potent Antitumor Agenta

1996 • 33 citations

IL 12 in acute viral infectious disease

1995 • 31 citations

Interleukin-12 in the pathogenesis and therapy of HIV disease

1995 • 20 citations

Systemic in vivo antitumor activity of interleukin-12 against both transplantable and primary tumor

1995 • 15 citations

The anti-tumor effects of IL-12 involve enhanced IFN-gamma production by anti-tumor T cells, their accumulation to tumor sites and in situ IFN-gamma production.

1997 • 13 citations

Interleukin‐12 Alters Helper T‐Cell Subsets and Antibody Profiles Induced by the Mucosal Adjuvant Cholera Toxin

1996 • 8 citations