Bioactive murine and human interleukin-12 fusion proteins which retain antitumor activity in vivo

Vaccination with irradiated tumor cells engineered to secrete murine granulocyte-macrophage colony-stimulating factor stimulates potent, specific, and long-lasting anti-tumor immunity.

1993 • 2,828 citations

Production of high-titer helper-free retroviruses by transient transfection.

1993 • 2,280 citations

Protein engineering of antibody binding sites: recovery of specific activity in an anti-digoxin single-chain Fv analogue produced in Escherichia coli.

1988 • 1,536 citations

Antitumor and antimetastatic activity of interleukin 12 against murine tumors.

1993 • 1,206 citations

Interleukin-12: a cytokine produced by antigen-presenting cells with immunoregulatory functions in the generation of T-helper cells type 1 and cytotoxic lymphocytes

1994 • 1,066 citations

Safe and efficient generation of recombinant retroviruses with amphotropic and ecotropic host ranges.

1988 • 908 citations

Cloning of cDNA for natural killer cell stimulatory factor, a heterodimeric cytokine with multiple biologic effects on T and natural killer cells.

1991 • 861 citations

Recombinant IL-12 administration induces tumor regression in association with IFN-gamma production.

1994 • 796 citations

Coexpression of two distinct genes is required to generate secreted bioactive cytotoxic lymphocyte maturation factor.

1991 • 593 citations

Biological behavior of malignant melanoma cells correlated to their survival in vivo.

1975 • 586 citations

Cloning and expression of murine IL-12.

1992 • 474 citations

Mouse interleukin‐12 (IL‐12) p40 homodimer: a potent IL‐12 antagonist

1995 • 441 citations

Human IL-12 p40 homodimer binds to the IL-12 receptor but does not mediate biologic activity.

1995 • 407 citations

Administration of recombinant IL-12 to normal mice enhances cytolytic lymphocyte activity and induces production of IFN-γ in vivo

1994 • 373 citations

Effects of retroviral vector design on expression of human adenosine deaminase in murine bone marrow transplant recipients engrafted with genetically modified cells.

1995 • 357 citations

Fibroblasts genetically engineered to secrete interleukin 12 can suppress tumor growth and induce antitumor immunity to a murine melanoma in vivo.

1994 • 335 citations

The Encephalomyocarditis Virus Internal Ribosome Entry Site Allows Efficient Coexpression of Two Genes from a Recombinant Provirus in Cultured Cells and in Embryos

1991 • 333 citations

The interleukin‐12 subunit p40 specifically inhibits effects of the interleukin‐12 heterodimer

1993 • 292 citations

IL-2 receptor α chain (CD25JAC) expression defines a crucial stage in pre-B cell development

1994 • 284 citations

Effective eradication of established murine tumors with IL-12 gene therapy using a polycistronic retroviral vector.

1995 • 277 citations

Gene gun-mediated skin transfection with interleukin 12 gene results in regression of established primary and metastatic murine tumors.

1996 • 248 citations

The encephalomyocarditis virus internal ribosome entry site allows efficient coexpression of two genes from a recombinant provirus in cultured cells and in embryos.

1991 • 235 citations

Cell surface antigens of chemically induced sarcomas of the mouse. I. Murine leukemia virus-related antigens and alloantigens on cultured fibroblasts and sarcoma cells: description of a unique antigen on BALB/c Meth A sarcoma.

1977 • 231 citations

Cancer immunotherapy of established tumors with IL-12. Effective delivery by genetically engineered fibroblasts.

1995 • 206 citations

Amount of interleukin 12 available at the tumor site is critical for tumor regression.

1996 • 177 citations

Medical Applications of Single-Chain Antibodies

1993 • 174 citations

Administration of Interleukin 12 With Pulse Interleukin 2 and the Rapid and Complete Eradication of Murine Renal Carcinoma

1996 • 164 citations

Enhanced hematopoietic activity of a human granulocyte/macrophage colony-stimulating factor-interleukin 3 fusion protein.

1991 • 140 citations

Construction and Characterization of Retroviral Vectors Expressing Biologically Active Human Interleukin-12

1994 • 131 citations

CD4 T cells inhibit in vivo the CD8‐mediated immune response against murine colon carcinoma cells transduced with interleukin‐12 genes

1995 • 125 citations

Construction of a Double Recombinant Adenovirus Vector Expressing a Heterodimeric Cytokine:In VitroandIn VivoProduction of Biologically Active Interleukin-12

1996 • 111 citations

In vitro maturation of human neonatal CD4 T lymphocytes. I. Induction of IL-4-producing cells after long-term culture in the presence of IL-4 plus either IL-2 or IL-12.

1994 • 75 citations

Measurement of Human and Mouse Interleukin‐12

1995 • 65 citations

Induction of Antigen-Specific Tumor Immunity by Genetic and Cellular Vaccines against MAGE: Enhanced Tumor Protection by Coexpression of Granulocyte-Macrophage Colony-Stimulating Factor and B7-1

1996 • 64 citations

Equal transcription of wild-type and mutant p53 using bicistronic vectors results in the wild-type phenotype.

1994 • 48 citations

After Initial Setback, IL-12 Regaining Popularity

1996 • 40 citations

Characterization of anti-mouse IL-12 monoclonal antibodies and measurement of mouse IL-12 by ELISA

1996 • 38 citations

Relationship between the cellular resistance to friend murine leukemia virus infection and the expression of murine leukemia virus-gp70-related glycoprotein on cell surface of BALB/c-Fv-4wr mice

1986 • 33 citations

Structure-Function Analysis of the p35 Subunit of Mouse Interleukin 12

1995 • 32 citations

Induction of autoantibody responses to GM-CSF by hyperimmunization with an Id-GM-CSF fusion protein.

1995 • 27 citations

Current protocols in immunology. Volume 1

1992 • 4 citations