A Proteasome Cap Subunit Required for Spindle Pole Body Duplication in Yeast

Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

1970 • 253,849 citations

Molecular Cloning: A Laboratory Manual

2001 • 133,517 citations

Basic local alignment search tool

1990 • 87,358 citations

Short-Term Effects of Nose-Only Cigarette Smoke Exposure on Glutathione Redox Homeostasis, Cytochrome P450 1A1/2 and Respiratory Enzyme Activities in Mice Tissues

2013 • 47,043 citations

A simple method for displaying the hydropathic character of a protein

1982 • 22,471 citations

A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae.

1989 • 8,705 citations

Distantly related sequences in the alpha- and beta-subunits of ATP synthase, myosin, kinases and other ATP-requiring enzymes and a common nucleotide binding fold.

1982 • 5,284 citations

Analysis of the accuracy and implications of simple methods for predicting the secondary structure of globular proteins

1978 • 4,566 citations

Predicting Coiled Coils from Protein Sequences

1991 • 3,977 citations

Inhibitors of the proteasome block the degradation of most cell proteins and the generation of peptides presented on MHC class I molecules

1994 • 2,480 citations

A positive selection for mutants lacking orotidine-5′-phosphate decarboxylase activity in yeast: 5-fluoro-orotic acid resistance

1984 • 2,401 citations

Guide to Yeast Genetics and Molecular Biology

1993 • 2,377 citations

Cyclin is degraded by the ubiquitin pathway

1991 • 2,355 citations

The ubiquitinproteasome pathway is required for processing the NF-κB1 precursor protein and the activation of NF-κB

1994 • 2,124 citations

Mammalian Ras interacts directly with the serine/threonine kinase raf

1993 • 1,983 citations

In Vivo Half-Life of a Protein Is a Function of Its Amino-Terminal Residue

1986 • 1,942 citations

The ubiquitin-proteasome proteolytic pathway

1994 • 1,778 citations

A knowledge base for predicting protein localization sites in eukaryotic cells

1992 • 1,518 citations

Crystal Structure of the 20 S Proteasome from the Archaeon T. acidophilum at 3.4 Å Resolution

1995 • 1,513 citations

The two-hybrid system: a method to identify and clone genes for proteins that interact with a protein of interest.

1991 • 1,489 citations

[19] Targeting, disruption, replacement, and allele rescue: Integrative DNA transformation in yeast

1991 • 1,369 citations

How Proteolysis Drives the Cell Cycle

1996 • 1,277 citations

The molecular biology of the yeast saccharomyces

1982 • 1,165 citations

The role of cyclin synthesis and degradation in the control of maturation promoting factor activity

1989 • 1,114 citations

A 20s complex containing CDC27 and CDC16 catalyzes the mitosis-specific conjugation of ubiquitin to cyclin B

1995 • 964 citations

Ubiquitin-dependent c-Jun degradation in vivo is mediated by the δ domain

1994 • 930 citations

Cyclin‐dependent protein kinases: Key regulators of the eukaryotic cell cycle

1995 • 918 citations

Ubiquitin, proteasomes, and the regulation of intracellular protein degradation

1995 • 862 citations

Structural rearrangements of tubulin and actin during the cell cycle of the yeast Saccharomyces.

1984 • 827 citations

Ubiquitination of a Yeast Plasma Membrane Receptor Signals Its Ligand-Stimulated Endocytosis

1996 • 789 citations

Replacement of chromosome segments with altered DNA sequences constructed in vitro.

1979 • 779 citations

Anaphase initiation in Saccharomyces cerevisiae is controlled by the APC-dependent degradation of the anaphase inhibitor Pds1p.

1996 • 777 citations

A 26 S protease subunit that binds ubiquitin conjugates.

1994 • 759 citations

The cyclosome, a large complex containing cyclin-selective ubiquitin ligase activity, targets cyclins for destruction at the end of mitosis.

1995 • 745 citations

Macromolecule Synthesis in Temperature-sensitive Mutants of Yeast

1967 • 728 citations

Anaphase is initiated by proteolysis rather than by the inactivation of maturation-promoting factor

1993 • 569 citations

Genes involved in sister chromatid separation are needed for b-type cyclin proteolysis in budding yeast

1995 • 561 citations

A novel genetic system to detect protein-protein interactions

1991 • 532 citations

MHC Class I Expression in Mice Lacking the Proteasome Subunit LMP-7

1994 • 520 citations

Destruction of the CDC28/CLB mitotic kinase is not required for the metaphase to anaphase transition in budding yeast.

1993 • 483 citations

The multicatalytic and 26 S proteases.

1993 • 476 citations

Cut2 proteolysis required for sister-chromatid separation in fission yeast

1996 • 451 citations

S. cerevisiae 26S protease mutants arrest cell division in G2/metaphase

1993 • 422 citations

Functions of microtubules in the Saccharomyces cerevisiae cell cycle.

1988 • 410 citations

Distinct 19 S and 20 S subcomplexes of the 26 S proteasome and their distribution in the nucleus and the cytoplasm.

1994 • 395 citations

KAR1, a gene required for function of both intranuclear and extranuclear microtubules in yeast

1987 • 372 citations

CDC27Hs colocalizes with CDC16Hs to the centrosome and mitotic spindle and is essential for the metaphase to anaphase transition

1995 • 369 citations

Pds1p, an inhibitor of anaphase in budding yeast, plays a critical role in the APC and checkpoint pathway(s).

1996 • 357 citations

Heat-Inducible Degron: a Method for Constructing Temperature-Sensitive Mutants

1994 • 349 citations

MPS1 and MPS2: novel yeast genes defining distinct steps of spindle pole body duplication.

1991 • 329 citations

Yeast gene required for spindle pole body duplication: homology of its product with Ca2+-binding proteins.

1986 • 321 citations

Proteasomes: protein degradation machines of the cell

1994 • 320 citations

The ABC-transporter Ste6 accumulates in the plasma membrane in a ubiquitinated form in endocytosis mutants.

1994 • 319 citations

A cyclin B homolog in S. cerevisiae: Chronic activation of the Cdc28 protein kinase by cyclin prevents exit from mitosis

1991 • 310 citations

Cyclin Ubiquitination: The destructive end of mitosis

1995 • 304 citations

Functions of the Proteasome: the Lysis at the End of the Tunnel

1995 • 302 citations

Cyclin B2 undergoes cell cycle-dependent nuclear translocation and, when expressed as a non-destructible mutant, causes mitotic arrest in HeLa cells

1992 • 298 citations

Cytology of the Yeast Life Cycle

1981 • 295 citations

Structure of the leucine zipper

1992 • 282 citations

Regulated degradation of the transcription factor Gcn4.

1994 • 271 citations

Identification of Subunits of the Anaphase-Promoting Complex of Saccharomyces cerevisiae

1996 • 263 citations

Control of Cell Fate by a Deubiquitinating Enzyme Encoded by the fat facets Gene

1995 • 262 citations

Selective protein degradation: A journey's end within the proteasome

1995 • 259 citations

Defective mitosis due to a mutation in the gene for a fission yeast 26S protease subunit

1993 • 244 citations

Cdc16p, Cdc23p and Cdc27p form a complex essential for mitosis.

1994 • 243 citations

In vivo degradation of a transcriptional regulator: The yeast α2 repressor

1990 • 243 citations

The short-lived MAT alpha 2 transcriptional regulator is ubiquitinated in vivo.

1991 • 242 citations

Identification, purification, and characterization of a high molecular weight, ATP-dependent activator (PA700) of the 20 S proteasome.

1994 • 238 citations

Components of a system that ligates cyclin to ubiquitin and their regulation by the protein kinase cdc2.

1994 • 235 citations

Red light-induced formation of ubiquitin-phytochrome conjugates: Identification of possible intermediates of phytochrome degradation

1987 • 224 citations

Pds1p is required for faithful execution of anaphase in the yeast, Saccharomyces cerevisiae

1996 • 219 citations

Microbial Determinations by Flow Cytometry

1979 • 203 citations

Identification of BIME as a Subunit of the Anaphase-Promoting Complex

1996 • 200 citations

Alterations in a yeast protein resembling HIV Tat-binding protein relieve requirement for an acidic activation domain in GAL4

1992 • 196 citations

Both cyclin A delta 60 and B delta 97 are stable and arrest cells in M-phase, but only cyclin B delta 97 turns on cyclin destruction.

1991 • 196 citations

Two complementary approaches to study peroxisome biogenesis in Saccharomyces cerevisiae: Forward and reversed genetics

1993 • 176 citations

Proteolytic processing of ovalbumin and beta-galactosidase by the proteasome to a yield antigenic peptides.

1994 • 172 citations

Subunit 4 of the 26 S protease is a member of a novel eukaryotic ATPase family

1992 • 171 citations

Two microtubule-associated proteins required for anaphase spindle movement in Saccharomyces cerevisiae [published erratum appears in J Cell Biol 1995 Oct;131(2):561]

1995 • 171 citations

Identification of the gal4 suppressor Sug1 as a subunit of the yeast 26S proteasome

1996 • 165 citations

A multicomponent system that degrades proteins conjugated to ubiquitin. Resolution of factors and evidence for ATP-dependent complex formation.

1988 • 161 citations

Proteolytic processing of ovalbumin and beta-galactosidase by the proteasome to a yield antigenic peptides.

1994 • 160 citations

Degradation of G alpha by the N-end rule pathway

1994 • 154 citations

Developmental Changes of the 26 S Proteasome in Abdominal Intersegmental Muscles of Manduca sexta during Programmed Cell Death

1995 • 152 citations

Cyclin-like accumulation and loss of the putative kinetochore motor CENP-E results from coupling continuous synthesis with specific degradation at the end of mitosis.

1994 • 151 citations

NDC1: a nuclear periphery component required for yeast spindle pole body duplication

1993 • 151 citations

Identification, Purification, and Characterization of a PA700-dependent Activator of the Proteasome

1996 • 138 citations

A gene required for the separation of chromosomes on the spindle apparatus in yeast

1986 • 130 citations

An efficient site-directed mutagenesis method based on PCR.

1994 • 129 citations

Separation of Sister Chromatids in Mitosis Requires the Drosophila pimples Product, a Protein Degraded after the Metaphase/Anaphase Transition

1996 • 125 citations

Identification of a set of yeast genes coding for a novel family of putative ATPases with high similarity to constituents of the 26S protease complex

1994 • 117 citations

Biogenesis, structure and function of the yeast 20S proteasome.

1995 • 115 citations

Copy number control by a yeast centromere

1983 • 113 citations

bendless, a Drosophila gene affecting neuronal connectivity, encodes a ubiquitin-conjugating enzyme homolog

1994 • 111 citations

Asymmetric mitotic segregation of the yeast spindle pole body

1992 • 97 citations

[41] Preparation of yeast cells for thin-section electron microscopy

1991 • 97 citations

Nin1p, a regulatory subunit of the 26S proteasome, is necessary for activation of Cdc28p kinase of Saccharomyces cerevisiae.

1995 • 94 citations

The dynamics of chromosome movement in the budding yeast Saccharomyces cerevisiae.

1989 • 86 citations

The Yeast SEN3 Gene Encodes a Regulatory Subunit of the 26S Proteasome Complex Required for Ubiquitin-Dependent Protein Degradation In Vivo

1995 • 74 citations

Cycloheximide-resistant temperature-sensitive lethal mutations of Saccharomyces cerevisiae.

1988 • 73 citations

Isolation and Characterization of SUG2

1996 • 64 citations

Mammalian Sug1 and c-Fos in the nuclear 26S proteasome.

1996 • 56 citations

Regulatory Proteins of the Proteasome

1993 • 47 citations

Studies on the Yeast Proteasome Uncover Its Basic Structural Features and Multiple in vivo Functions

1993 • 41 citations

cDNA cloning of p42, a shared subunit of two proteasome regulatory proteins, reveals a novel member of the AAA protein family

1996 • 27 citations

Molecular Genetics of the Ubiquitin System

1988 • 17 citations