Distinct 19 S and 20 S subcomplexes of the 26 S proteasome and their distribution in the nucleus and the cytoplasm.

Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

1979 • 54,907 citations

Continuous cultures of fused cells secreting antibody of predefined specificity

1975 • 17,037 citations

Improved silver staining of plant proteins, RNA and DNA in polyacrylamide gels

1987 • 4,186 citations

A method for the quantitative recovery of protein in dilute solution in the presence of detergents and lipids

1984 • 3,959 citations

High resolution two-dimensional electrophoresis of basic as well as acidic proteins

1977 • 3,556 citations

Oogenesis in Xenopus laevis (Daudin). I. Stages of oocyte development in laboratory maintained animals

1972 • 1,861 citations

THE UBIQUITIN SYSTEM FOR PROTEIN DEGRADATION

1992 • 1,402 citations

Ornithine decarboxylase is degraded by the 26S proteasome without ubiquitination

1992 • 780 citations

Proposed role of ATP in protein breakdown: conjugation of protein with multiple chains of the polypeptide of ATP-dependent proteolysis.

1980 • 727 citations

An octamer of histones in chromatin and free in solution.

1975 • 725 citations

Widespread occurrence of intermediate-sized filaments of the vimentin-type in cultured cells from diverse vertebrates

1979 • 642 citations

Proteolysis, proteasomes and antigen presentation

1992 • 576 citations

THE UBIQUITIN-CONJUGATION SYSTEM

1992 • 561 citations

Purification of two high molecular weight proteases from rabbit reticulocyte lysate.

1987 • 542 citations

ATP-dependent conjugation of reticulocyte proteins with the polypeptide required for protein degradation.

1980 • 530 citations

The multicatalytic and 26 S proteases.

1993 • 476 citations

The mechanism and functions of ATP‐dependent proteases in bacterial and animal cells

1992 • 450 citations

Ubiquitination

1991 • 430 citations

Proteinase yscE, the yeast proteasome/multicatalytic-multifunctional proteinase: mutants unravel its function in stress induced proteolysis and uncover its necessity for cell survival.

1991 • 421 citations

Fluorimetric assays for cathepsin B and cathepsin H with methylcoumarylamide substrates

1980 • 404 citations

Identity of the 19S 'prosome' particle with the large multifunctional protease complex of mammalian cells (the proteasome)

1988 • 404 citations

Proteasomes: multicatalytic proteinase complexes

1993 • 395 citations

ATP-dependent incorporation of 20S protease into the 26S complex that degrades proteins conjugated to ubiquitin.

1989 • 370 citations

Degradation of nuclear oncoproteins by the ubiquitin system in vitro.

1991 • 345 citations

The proteasome (multicatalytic protease) is a component of the 1500-kDa proteolytic complex which degrades ubiquitin-conjugated proteins.

1990 • 327 citations

A role for the ubiquitin-dependent proteolytic pathway in MHC class l-restricted antigen presentation

1993 • 317 citations

Abnormally high expression of proteasomes in human leukemic cells.

1990 • 305 citations

An abundant and ubiquitous homo-oligomeric ring-shaped ATPase particle related to the putative vesicle fusion proteins Sec18p and NSF.

1990 • 279 citations

Ubiquitin-lysozyme conjugates. Identification and characterization of an ATP-dependent protease from rabbit reticulocyte lysates.

1986 • 278 citations

The multicatalytic proteinase (prosome) is ubiquitous from eukaryotes to archaebacteria

1989 • 277 citations

PRE2, highly homologous to the human major histocompatibility complex-linked RING10 gene, codes for a yeast proteasome subunit necessary for chrymotryptic activity and degradation of ubiquitinated proteins.

1993 • 260 citations

Structural Features of the 26 S Proteasome Complex

1993 • 255 citations

Demonstration of two distinct high molecular weight proteases in rabbit reticulocytes, one of which degrades ubiquitin conjugates.

1987 • 255 citations

Natural substrates of the Ubiquitin proteolytic pathway

1991 • 238 citations

Primary structure of the Thermoplasma proteasome and its implications for the structure, function, and evolution of the multicatalytic proteinase

1992 • 235 citations

Multiple forms of the 20 S multicatalytic and the 26 S ubiquitin/ATP-dependent proteases from rabbit reticulocyte lysate.

1992 • 229 citations

Subunit 4 of the 26 S protease is a member of a novel eukaryotic ATPase family

1992 • 171 citations

Spontaneous losses of control of cytokeratin gene expression in transformed, non-epithelial human cells occurring at different levels of regulation

1989 • 167 citations

Nucleocytoplasmic segregation of proteins and RNAs

1983 • 163 citations

A multicomponent system that degrades proteins conjugated to ubiquitin. Resolution of factors and evidence for ATP-dependent complex formation.

1988 • 161 citations

Soluble acidic complexes containing histones H3 and H4 in nuclei of Xenopus laevis oocytes

1982 • 158 citations

The PRE4 gene codes for a subunit of the yeast proteasome necessary for peptidylglutamyl-peptide-hydrolyzing activity. Mutations link the proteasome to stress- and ubiquitin-dependent proteolysis.

1993 • 149 citations

In vivo function of the proteasome in the ubiquitin pathway.

1992 • 144 citations

Electron microscopic localization of the multicatalytic proteinase complex in rat liver and in cultured cells.

1992 • 144 citations

ATP‐dependent reversible association of proteasomes with multiple protein components to form 26S complexes that degrade ubiquitinated proteins in human HL‐60 cells

1991 • 141 citations

The conserved carboxy-terminal cysteine of nuclear lamins is essential for lamin association with the nuclear envelope.

1989 • 134 citations

Possible mechanism of nuclear translocation of proteasomes

1990 • 132 citations

A major soluble acidic protein located in nuclei of diverse vertebrate species

1980 • 131 citations

Changes in intracellular localization of proteasomes in immortalized ovarian granulosa cells during mitosis associated with a role in cell cycle control.

1993 • 122 citations

Demonstration that a human 26S proteolytic complex consists of a proteasome and multiple associated protein components and hydrolyzes ATP and ubiquitin‐ligated proteins by closely linked mechanisms

1992 • 115 citations

Regulation of gene expression of proteasomes (multi-protease complexes) during growth and differentiation of human hematopoietic cells.

1992 • 111 citations

Ubiquitous soluble Mg2+-ATPase complex

1992 • 109 citations

Proteasomes: protein and gene structures.

1992 • 108 citations

Purification and characterization of a multiprotein component of the Drosophila 26 S (1500 kDa) proteolytic complex.

1993 • 108 citations

The PROS-35 gene encodes the 35 kd protein subunit of Drosophila melanogaster proteasome.

1989 • 89 citations

Purification and Characterization of the 26S Proteasome Complex Catalyzing ATP-Dependent Breakdown of Ubiquitin-Ligated Prot from Rat Liver1

1993 • 85 citations

Identification of a widespread nuclear actin binding protein

1989 • 85 citations

Peptide sequencing identifies MSS1, a modulator of HIV Tat‐mediated transactivation, as subunit 7 of the 26 S protease

1993 • 84 citations

Karyophilic proteins: polypeptides synthesized in vitro accumulate in the nucleus on microinjection into the cytoplasm of amphibian oocytes.

1982 • 80 citations

The association of prosomes with some of the intermediate filament networks of the animal cell.

1988 • 77 citations

The 22 S cylinder particles of Xenopus laevis. I. Biochemical and electron microscopic characterization.

1983 • 77 citations

The proteasome/multicatalytic—multifunctional proteinase In vivo function in the ubiquitin‐dependent N‐end rule pathway of protein degradation in eukaryotes

1992 • 76 citations

Immunoelectron microscopic localization of ubiquitin in hepatoma cells.

1988 • 74 citations

Cytolocalization of prosomes as a function of differentiation

1988 • 73 citations

Presence And Distribution Of Specific Prosome Antigens Change As A Function Of Embryonic Development And Tissue-Type Differentiation In Pleurodeles Waltl

1988 • 71 citations

Identification and localization of a novel nucleolar protein of high molecular weight by a monoclonal antibody

1984 • 70 citations

Direct evidence for nuclear and cytoplasmic colocalization of proteasomes (Multiprotease complexes) in liver

1989 • 69 citations

Cell-specific accumulation of Drosophila proteasomes (MCP) during early development.

1990 • 69 citations

Microinjection of ubiquitin: intracellular distribution and metabolism in HeLa cells maintained under normal physiological conditions.

1987 • 64 citations

Large scale isolation of nuclei and nucleoli from vitellogenic oocytes of Xenopus laevis

1978 • 60 citations

Sea urchin prosome: characterization and changes during development.

1987 • 59 citations

Purification of proteasomes from salmonid fish sperm and their localization along sperm flagella

1993 • 58 citations

Diffusive and nondiffusive proteins in vivo.

1984 • 56 citations

Immunoelectron microscopic localization of the ubiquitin-activating enzyme E1 in HepG2 cells.

1992 • 56 citations

Mos is degraded by the 26S proteasome in a ubiquitin‐dependent fashion

1993 • 56 citations

Cell cycle-dependent change of proteasome distribution during embryonic development of the ascidian Halocynthia roretzi

1992 • 55 citations

Ultrastructure of the approximately 26S complex containing the approximately 20S cylinder particle (multicatalytic proteinase/proteasome).

1991 • 54 citations

The 22 S cylinder particles of Xenopus laevis. II. Immunological characterization and localization of their proteins in tissues and cultured cells.

1983 • 49 citations

The 19S ring-type particles of Drosophila

1987 • 46 citations

Endogenous inhibitor of nonlysosomal high molecular weight protease and calcium-dependent protease.

1986 • 44 citations

Electron microscopy of 26 S complex containing 20 S proteasome

1991 • 41 citations

Differential Cytolocalization Of Prosomes In Axolotl During Oogenesis And Meiotic Maturation

1988 • 40 citations

An ATP-stabilized inhibitor of the proteasome is a component of the 1500-kDa ubiquitin conjugate-degrading complex.

1992 • 40 citations

Proteasome location

1993 • 33 citations

Specific types of prosomes are associated to subnetworks of the intermediate filaments in PtK1 cells.

1992 • 27 citations

Molecular interaction of the proteasome (multicatalytic proteinase). Evidence that the proteasome is not a constituent of the ‘26 S’ multienzyme complex

1991 • 24 citations

Immunocytochemical localization of the multicatalytic proteinase (Proteasome) in crustacean striated muscles

1992 • 23 citations

Immunocytochemical localization of ubiquitin-activating enzyme in the cell nucleus

1991 • 21 citations

Immunolocalization of a specific type of prosome close to the bile canaliculi in fetal and adult rat liver.

1992 • 21 citations

Ubiquitinated proteasome inhibitor is a component of the 26 S proteasome complex

1992 • 19 citations

Evidence indicating that the multicatalytic proteinase of rabbit reticulocytes is not incorporated as a core enzyme into a 26 S proteinase complex

1992 • 10 citations

Localization of a sequence motif complementary to the nuclear localization signal in proteasomes from Thermoplasma acidophilum by immunoelectron microscopy

1992 • 9 citations