Assembly of the Cleavage and Polyadenylation Apparatus Requires About 10 Seconds In Vivo and Is Faster for Strong than for Weak Poly(A) Sites

Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells.

1982 • 8,069 citations

Current protocols in molecular biology

1988 • 4,543 citations

SV40-transformed simian cells support the replication of early SV40 mutants

1981 • 2,564 citations

The Rous sarcoma virus long terminal repeat is a strong promoter when introduced into a variety of eukaryotic cells by DNA-mediated transfection.

1982 • 1,371 citations

Mechanical Devices of the Spliceosome: Motors, Clocks, Springs, and Things

1998 • 1,121 citations

The C-terminal domain of RNA polymerase II couples mRNA processing to transcription

1997 • 905 citations

Improved predictions of secondary structures for RNA.

1989 • 822 citations

Mechanism and regulation of mRNA polyadenylation

1997 • 762 citations

Factors involved in specific transcription by human RNA polymerase II: analysis by a rapid and quantitative in vitro assay.

1985 • 575 citations

RNA as a target of double-stranded RNA-mediated genetic interference in Caenorhabditis elegans

1998 • 561 citations

Splice site selection, rate of splicing, and alternative splicing on nascent transcripts.

1988 • 539 citations

Stepwise assembly of a pre-mRNA splicing complex requires U-snRNPs and specific intron sequences

1985 • 524 citations

Steps in the processing of Ad2 mRNA: Poly(A)+ Nuclear sequences are conserved and poly(A) addition precedes splicing

1978 • 478 citations

A functional mRNA polyadenylation signal is required for transcription termination by RNA polymerase II.

1988 • 370 citations

The human dystrophin gene requires 16 hours to be transcribed and is cotranscriptionally spliced

1995 • 361 citations

RNA polymerase II is an essential mRNA polyadenylation factor

1998 • 358 citations

How RNA polymerase II terminates transcription in higher eukaryotes

1989 • 301 citations

Transcription factor TFIID recruits factor CPSF for formation of 3′ end of mRNA

1997 • 301 citations

Definition of an efficient synthetic poly(A) site.

1989 • 285 citations

The 64-kilodalton subunit of the CstF polyadenylation factor binds to pre-mRNAs downstream of the cleavage site and influences cleavage site location.

1994 • 236 citations

Position-dependent sequence elements downstream of AAUAAA are required for efficient rabbit β-globin mRNA 3′ end formation

1987 • 229 citations

Efficiency of Utilization of the Simian Virus 40 Late Polyadenylation Site: Effects of Upstream Sequences

1989 • 205 citations

Separation and characterization of a poly(A) polymerase and a cleavage/specificity factor required for pre-mRNA polyadenylation

1988 • 195 citations

An ordered pathway of assembly of components required for polyadenylation site recognition and processing.

1989 • 194 citations

Cleavage site determinants min the mammalian polydenylation signal

1995 • 186 citations

Sequences capable of restoring poly(A) site function define two distinct downstream elements.

1986 • 175 citations

A systematic analysis of the factors that determine the strength of pre-mRNA splicing enhancers

1998 • 168 citations

Functional interactions of the simian virus 40 core origin of replication with flanking regulatory sequences

1986 • 166 citations

A method for increasing the sensitivity of chloramphenicol acetyltransferase assays in extracts of transfected cultured cells

1987 • 161 citations

The upstream sequence element of the C2 complement poly(A) signal activates mRNA 3′ end formation by two distinct mechanisms

1998 • 156 citations

Polypyrimidine Tract-Binding Protein Positively Regulates Inclusion of an Alternative 3′-Terminal Exon

1999 • 150 citations

Direct interaction of the U1 snRNP-A protein with the upstream efficiency element of the SV40 late polyadenylation signal.

1994 • 146 citations

Poly(A) site efficiency reflects the stability of complex formation involving the downstream element.

1991 • 140 citations

3′-End cleavage and polyadenylation of mRNA precursors

1995 • 136 citations

CPSF recognition of an HIV-1 mRNA 3'-processing enhancer: multiple sequence contacts involved in poly(A) site definition.

1995 • 128 citations

The HIV-1 5' LTR poly(A) site is inactivated by U1 snRNP interaction with the downstream major splice donor site

1997 • 111 citations

Poly(A) polymerase purified from HeLa cell nuclear extract is required for both cleavage and polyadenylation of pre-mRNA in vitro.

1989 • 104 citations

The HTLV-I rex response element mediates a novel form of mRNA polyadenylation

1991 • 101 citations

A complex protein assembly catalyzes polyadenylation of mRNA precursors

1995 • 90 citations

The Mechanism of 3′ Cleavage and Polyadenylation of Eukaryotic Pre-mRNA1

1997 • 88 citations

Activation of HIV-1 pre-mRNA 3′ processing in vitro requires both an upstream element and TAR.

1992 • 87 citations

Transcriptional termination in the Balbiani ring 1 gene is closely coupled to 3′-end formation and excision of the 3′-terminal intron

1998 • 87 citations

The G-rich auxiliary downstream element has distinct sequence and position requirements and mediates efficient 3′ end pre-mRNA processing through atrans-acting factor

1995 • 83 citations

Definition of the Upstream Efficiency Element of the Simian Virus 40 Late Polyadenylation Signal by Using In Vitro Analyses

1992 • 81 citations

An Intron Enhancer Containing a 5′ Splice Site Sequence in the Human Calcitonin/Calcitonin Gene-Related Peptide Gene

1995 • 74 citations

3' RNA processing efficiency plays a primary role in generating termination-competent RNA polymerase II elongation complexes.

1993 • 71 citations

Pre-Mrna Processing

1995 • 68 citations

Regulation of polyadenylation in hepatitis B viruses: stimulation by the upstream activating signal PS1 is orientation-dependent, distance-independent, and additive

1991 • 59 citations

RNA Structure Is a Critical Determinant of Poly(A) Site Recognition by Cleavage and Polyadenylation Specificity Factor

1996 • 58 citations

Auxiliary downstream elements are required for efficient polyadenylation of mammalian pre-mRNAs

1998 • 54 citations

Patterns of polyadenylation site selection in gene constructs containing multiple polyadenylation signals.

1988 • 53 citations

Addition of poly(A) to nuclear RNA occurs soon after RNA synthesis.

1980 • 46 citations

Targeted nuclear antisense RNA mimics natural antisense-induced degradation of polyoma virus early RNA.

1994 • 44 citations

Kinetics and efficiency of polyadenylation of late polyomavirus nuclear RNA: generation of oligomeric polyadenylated RNAs and their processing into mRNA.

1984 • 42 citations

Sequence elements upstream of the 3' cleavage site confer substrate strength to the adenovirus L1 and L3 polyadenylation sites.

1994 • 40 citations

Effect of RNA secondary structure on polyadenylation site selection.

1991 • 40 citations

Transcription and polyadenylation in a short human intergenic region

1997 • 37 citations

Analysis of Premature Termination in c-myc during Transcription by RNA Polymerase II in a HeLa Nuclear Extract

1991 • 32 citations

A common mechanism for the enhancement of mRNA 3' processing by U3 sequences in two distantly related lentiviruses

1996 • 31 citations

Transcription termination at the chicken beta H-globin gene.

1988 • 30 citations

Upstream and downstream c/s-acting elements for cleavage at the L4 polyadenylation site of adenovirus-2

1994 • 26 citations

Alterations in the pre-mRNA topology of the bovine growth hormone polyadenylation region decrease poly(A) site efficiency

1989 • 21 citations

Poly(A) site selection in the yeast Ty retroelement requires an upstream region and sequence-specific titratable factor(s) in vitro.

1994 • 21 citations

Sequences regulating poly(A) site selection within the adenovirus major late transcription unit influence the interaction of constitutive processing factors with the pre-mRNA

1996 • 20 citations

Poly(A)-Driven and Poly(A)-Assisted Termination: Two Different Modes of Poly(A)-Dependent Transcription Termination

1998 • 20 citations

Splicing and polyadenylylation at cryptic sites in RNA transcribed from pSV2-neo

1986 • 12 citations

3′-End Cleavage and polyadenylation of nuclear Messenger RNA Precursors

1995 • 9 citations

DNase I Treatment of Total RNA Improves the Accuracy of Ribonuclease Protection Assay

1998 • 6 citations