PEB1 (PAS7) in Saccharomyces cerevisiae encodes a hydrophilic, intra-peroxisomal protein that is a member of the WD repeat family and is essential for the import of thiolase into peroxisomes.

Molecular Cloning: A Laboratory Manual

2001 • 133,517 citations

DNA sequencing with chain-terminating inhibitors

1977 • 69,181 citations

A simple method for displaying the hydropathic character of a protein

1982 • 22,471 citations

Antibodies, a laboratory manual

1989 • 12,929 citations

A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae.

1989 • 8,705 citations

Unidirectional digestion with exonuclease III creates targeted breakpoints for DNA sequencing

1984 • 4,231 citations

High efficiency transformation of E.coli by high voltage electroporation

1988 • 2,789 citations

A ten-minute DNA preparation from yeast efficiently releases autonomous plasmids for transformaion of Escherichia coli

1987 • 2,493 citations

Two differentially regulated mRNAs with different 5′ ends encode secreted and intracellular forms of yeast invertase

1982 • 1,539 citations

The ancient regulatory-protein family of WD-repeat proteins

1994 • 1,478 citations

[19] Targeting, disruption, replacement, and allele rescue: Integrative DNA transformation in yeast

1991 • 1,369 citations

A Saccharomyces cerevisiae genomic plasmid bank based on a centromere-containing shuttle vector

1987 • 1,181 citations

A conserved tripeptide sorts proteins to peroxisomes.

1989 • 1,113 citations

Biogenesis of Peroxisomes

1985 • 1,108 citations

BIOCHEMISTRY OF PEROXISOMES

1992 • 843 citations

The structure of an antigenic determinant in a protein

1984 • 841 citations

Requirement for hsp70 in the mitochondrial matrix for translocation and folding of precursor proteins

1990 • 756 citations

Tissue fractionation studies. 17. Intracellular distribution of monoamine oxidase, aspartate aminotransferase, alanine aminotransferase, <scp>d</scp>-amino acid oxidase and catalase in rat-liver tissue

1964 • 649 citations

A novel, cleavable peroxisomal targeting signal at the amino-terminus of the rat 3-ketoacyl-CoA thiolase.

1991 • 629 citations

[35] Epitope tagging and protein surveillance

1991 • 526 citations

Two nuclear mutations that block mitochondrial protein import in yeast.

1984 • 490 citations

The TPR snap helix: a novel protein repeat motif from mitosis to transcription

1991 • 452 citations

Loss of BiP/GRP78 function blocks translocation of secretory proteins in yeast.

1990 • 435 citations

The Cln3-Cdc28 kinase complex of S. cerevisiae is regulated by proteolysis and phosphorylation.

1992 • 414 citations

Repetitive segmental structure of the transducin beta subunit: homology with the CDC4 gene and identification of related mRNAs.

1986 • 411 citations

Sec61p and BiP directly facilitate polypeptide translocation into the ER

1992 • 370 citations

Isolation of peroxisome-deficient mutants of Saccharomyces cerevisiae.

1989 • 326 citations

Proliferation of microbodies in Saccharomyces cerevisiae

1987 • 302 citations

PAS7 encodes a novel yeast member of the WD-40 protein family essential for import of 3-oxoacyl-CoA thiolase, a PTS2-containing protein, into peroxisomes.

1994 • 295 citations

Amino-terminal presequence of the precursor of peroxisomal 3-ketoacyl-CoA thiolase is a cleavable signal peptide for peroxisomal targeting

1991 • 288 citations

Saccharomyces cerevisiae peroxisomal thiolase is imported as a dimer.

1994 • 277 citations

A dual role for mitochondrial heat shock protein 70 in membrane translocation of preproteins.

1993 • 274 citations

FUS3 phosphorylates multiple components of the mating signal transduction cascade: evidence for STE12 and FAR1.

1993 • 274 citations

The pas8 mutant of Pichia pastoris exhibits the peroxisomal protein import deficiencies of Zellweger syndrome cells--the PAS8 protein binds to the COOH-terminal tripeptide peroxisomal targeting signal, and is a member of the TPR protein family [published erratum appears in J Cell Biol 1993 Sep;122(5):following 1143]

1993 • 246 citations

The WD‐40 repeat

1992 • 241 citations

Yeast promoters: Positive and negative elements

1984 • 227 citations

Identification of mutations associated with peroxisome-to-mitochondrion mistargeting of alanine/glyoxylate aminotransferase in primary hyperoxaluria type 1.

1990 • 226 citations

PAS10 is a tetratricopeptide-repeat protein that is essential for the import of most matrix proteins into peroxisomes of Saccharomyces cerevisiae.

1993 • 225 citations

PAS3, a Saccharomyces cerevisiae gene encoding a peroxisomal integral membrane protein essential for peroxisome biogenesis.

1991 • 218 citations

The CYC8 and TUP1 proteins involved in glucose repression in Saccharomyces cerevisiae are associated in a protein complex.

1991 • 216 citations

Acyl-CoA oxidase contains two targeting sequences each of which can mediate protein import into peroxisomes.

1988 • 196 citations

Peroxisome biogenesis revisited

1989 • 189 citations

Two complementary approaches to study peroxisome biogenesis in Saccharomyces cerevisiae: Forward and reversed genetics

1993 • 176 citations

Expression of heterologous genes in Saccharomyces cerevisiae from vectors utilizing the glyceraldehyde-3-phosphate dehydrogenase gene promoter

1984 • 174 citations

Evolutionary conservation of a microbody targeting signal that targets proteins to peroxisomes, glyoxysomes, and glycosomes.

1991 • 166 citations

Mutagenesis of the amino targeting signal of Saccharomyces cerevisiae 3-ketoacyl-CoA thiolase reveals conserved amino acids required for import into peroxisomes in vivo.

1994 • 160 citations

Two independent peroxisomal targeting signals in catalase A of Saccharomyces cerevisiae.

1993 • 158 citations

The processing peptidase of yeast mitochondria: the two co-operating components MPP and PEP are structurally related.

1988 • 152 citations

Import of proteins into yeast mitochondria: the nuclear MAS2 gene encodes a component of the processing protease that is homologous to the MAS1-encoded subunit.

1988 • 149 citations

Association of glyoxylate and beta-oxidation enzymes with peroxisomes of Saccharomyces cerevisiae

1990 • 136 citations

Partial disassembly of peroxisomes.

1985 • 130 citations

Isolation of peroxisome assembly mutants from Saccharomyces cerevisiae with different morphologies using a novel positive selection procedure.

1992 • 123 citations

Differential protein import deficiencies in human peroxisome assembly disorders.

1994 • 117 citations

Cloning of three human multifunctional de novo purine biosynthetic genes by functional complementation of yeast mutations.

1990 • 110 citations

Biosynthesis and maturation of peroxisomal beta-oxidation enzymes in fibroblasts in relation to the Zellweger syndrome and infantile Refsum disease.

1986 • 106 citations

Peroxisomal biogenesis: multiple pathways of protein import.

1994 • 97 citations

Rat liver peroxisomes catalyze the initial step in cholesterol synthesis. The condensation of acetyl-CoA units into acetoacetyl-CoA.

1990 • 93 citations

The 2.8å Crystal Structure of peroxisomal 3-ketoacyl-CoA thiolase of Saccharomyces cerevisiae : a five-layered αβαβα structure constructed from two core domains of identical topology

1994 • 85 citations

A Saccharomyces cerevisiae upstream activating sequence mediates induction of peroxisome proliferation by fatty acids

1993 • 80 citations

The Role of Peroxisomes in Cholesterol Metabolism

1992 • 71 citations

The peroxisomal targeting signal of 3‐oxoacyl‐coA thiolase from Saccharomyces cerevisiae

1994 • 70 citations

Peroxisomes in Saccharomyces cerevisiae: immunofluorescence analysis and import of catalase A into isolated peroxisomes.

1991 • 69 citations

Chapter 23 Transmission Electron Microscopy and Immunocytochemical Studies of Yeast: Analysis of HMG—CoA Reductase Overproduction by Electron Microscopy

1989 • 65 citations

Rhizomelic chondrodysplasia punctata. Deficiency of 3-oxoacyl-coenzyme A thiolase in peroxisomes and impaired processing of the enzyme.

1990 • 58 citations

Novel peroxisome clustering mutants and peroxisome biogenesis mutants of Saccharomyces cerevisiae.

1993 • 58 citations

Efficient association of in vitro translation products with purified stable Candida tropicalis peroxisomes.

1987 • 46 citations

Presence of cytoplasmic factors functional in peroxisomal protein import implicates organelle-associated defects in several human peroxisomal disorders.

1993 • 40 citations

The POX1 gene encoding peroxisomal acyl-CoA oxidase in Saccharomyces cerevisiae is under the control of multiple regulatory elements.

1994 • 34 citations

Three peroxisome protein packaging pathways suggested by selective permeabilization of yeast mutants defective in peroxisome biogenesis.

1993 • 30 citations

Samples, reagents, assessment of results

1983 • 25 citations

Transcriptional regulation of genes encoding proteins involved in biogenesis of peroxisomes in Saccharomyces cerevisiae

1992 • 22 citations

Peroxisomes in Saccharomyces cerevisiae: Immunofluorescence Analysis and Import of Catalase A into Isolated Peroxisomes

1991 • 20 citations

Chapter 14 Protein Import into Peroxisomes in Vitro

1991 • 17 citations

Efficient Association of in Vitro Translation Products With Purified Stable Candida Tropicalis Peroxisomes

1987 • 16 citations

Expression and Transport of Candida tropicalis Peroxisomal Acyl-coenzyme A Oxidase in the Yeast Candida maltosa

1989 • 14 citations

The processing peptidase of yeast mitochondria

1988 • 3 citations