Two independent peroxisomal targeting signals in catalase A of Saccharomyces cerevisiae.

Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

1970 • 253,849 citations

A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding

1976 • 225,752 citations

Molecular Cloning: A Laboratory Manual

2001 • 133,517 citations

Short-Term Effects of Nose-Only Cigarette Smoke Exposure on Glutathione Redox Homeostasis, Cytochrome P450 1A1/2 and Respiratory Enzyme Activities in Mice Tissues

2013 • 47,043 citations

Primer-Directed Enzymatic Amplification of DNA with a Thermostable DNA Polymerase

1988 • 17,059 citations

A rapid alkaline extraction procedure for screening recombinant plasmid DNA

1979 • 15,511 citations

“Western Blotting”: Electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A

1981 • 8,657 citations

Transformation of intact yeast cells treated with alkali cations

1983 • 7,688 citations

Site-directed mutagenesis by overlap extension using the polymerase chain reaction

1989 • 7,496 citations

Protein folding in the cell

1992 • 4,138 citations

Firefly luciferase gene: structure and expression in mammalian cells.

1987 • 3,088 citations

A subfamily of stress proteins facilitates translocation of secretory and mitochondrial precursor polypeptides

1988 • 1,504 citations

A RAPID ALKALINE EXTRACTION PROCEDURE FOR SCREENING RECOMBINANT DNA

1979 • 1,402 citations

Yeast/E. coli shuttle vectors with multiple unique restriction sites

1986 • 1,399 citations

A MODIFIED PROCEDURE FOR LEAD STAINING OF THIN SECTIONS

1961 • 1,388 citations

A conserved tripeptide sorts proteins to peroxisomes.

1989 • 1,113 citations

Biogenesis of Peroxisomes

1985 • 1,108 citations

Ultrastructural localization of intracellular antigens by the use of protein A-gold complex.

1978 • 992 citations

Firefly Luciferase Gene: Structure and Expression in Mammalian Cells

1987 • 924 citations

A reliable method for the recovery of DNA fragments from agarose and acrylamide gels

1981 • 916 citations

Resin development for electron microscopy and an analysis of embedding at low temperature*

1982 • 714 citations

[16] Plasmids of Escherichia coli as cloning vectors

1979 • 655 citations

A novel, cleavable peroxisomal targeting signal at the amino-terminus of the rat 3-ketoacyl-CoA thiolase.

1991 • 629 citations

How proteins enter the nucleus

1991 • 555 citations

Protein Translocation Across Membranes

1988 • 482 citations

Identification of a peroxisomal targeting signal at the carboxy terminus of firefly luciferase.

1987 • 481 citations

Structure of beef liver catalase

1981 • 426 citations

The active center of catalase

1985 • 420 citations

Identification of peroxisomal targeting signals located at the carboxy terminus of four peroxisomal proteins.

1988 • 338 citations

Peroxisomal protein import is conserved between yeast, plants, insects and mammals.

1990 • 296 citations

How do polypeptides cross the mitochondrial membranes?

1990 • 246 citations

Peroxisome biogenesis revisited

1989 • 189 citations

In situ hybridization at the electron microscope level: localization of transcripts on ultrathin sections of Lowicryl K4M-embedded tissue using biotinylated probes and protein A-gold complexes.

1986 • 164 citations

Three-dimensional structure of catalase from Penicillium vitale at 2.0 Å resolution

1986 • 158 citations

Subunit IV of yeast cytochrome c oxidase: cloning and nucleotide sequencing of the gene and partial amino acid sequencing of the mature protein.

1984 • 154 citations

The Saccharomyces cerevisiae ADR1 gene is a positive regulator of transcription of genes encoding peroxisomal proteins.

1991 • 150 citations

Rat peroxisomal 3-ketoacyl-CoA thiolase gene. Occurrence of two closely related but differentially regulated genes.

1990 • 131 citations

Citrate synthase encoded by the CIT2 gene of Saccharomyces cerevisiae is peroxisomal.

1990 • 120 citations

Characterization of catalase transcripts and their differential expression in maize

1988 • 112 citations

The complete amino acid sequence of bovine liver catalase and the partial sequence of bovine erythrocyte catalase

1982 • 111 citations

Immunolocalization of glyceraldehyde-3-phosphate dehydrogenase, hexokinase, and carboxypeptidase Y in yeast cells at the ultrastructural level.

1987 • 109 citations

Mitochondrial and Nonmitochondrial Citrate Synthases in Saccharomyces cerevisiae Are Encoded by Distinct Homologous Genes

1986 • 109 citations

Sequence of the Saccharomyces cerevisiae CATI gene and amino acid sequence of catalase A derived from it

1988 • 104 citations

The carboxyl-terminal tripeptide Ala-Lys-Ile is essential for targeting Candida tropicalis trifunctional enzyme to yeast peroxisomes.

1991 • 97 citations

Isolation of human fibroblast catalase cDNA clones. Sequence of clones derived from spliced and unspliced mRNA.

1984 • 90 citations

Recognition of nascent polypeptides for targeting and folding

1991 • 78 citations

Isolation of the catalase A gene of Saccharomyces cerevisiae by complementation of the cta1 mutation

1985 • 74 citations

Cloning and sequence determination of cDNA encoding a second rat liver peroxisomal 3-ketoacyl-CoA thiolase

1990 • 64 citations

Targeting signal of the peroxisomal catalase in the methylotrophic yeastHansenula polymorpha

1992 • 62 citations

Nucleotide sequence of human peroxisomal 3-oxoacyl-CoA thiolase

1988 • 61 citations

Nitrocellulose-bound antigen repeatedly used for the affinity purification of specific polyclonal antibodies for screening DNA expression libraries

1988 • 60 citations

Targeting sequences of the two major peroxisomal proteins in the methylotrophic yeast Hansenula polymorpha

1992 • 56 citations

The Saccharomyces cerevisiae ADR1 Gene Is a Positive Regulator of Transcription of Genes Encoding Peroxisomal Proteins

1991 • 41 citations

Citrate Synthase Encoded by the CIT2 Gene of Saccharomyces cerevisiae Is Peroxisomal

1990 • 35 citations

The amino acid sequence of dihydrofolate reductase from L1210 cells

1977 • 32 citations

Vector-mediated overexpression of catalase A in the yeast Saccharomyces cerevisiae induces inclusion body formation.

1991 • 28 citations

cDNA and deduced amino acid sequence ofDrosophilacatalase

1990 • 28 citations

Fate of highly expressed proteins destined to peroxisomes in Saccharomyces cerevisiae

1990 • 22 citations