FUS3 phosphorylates multiple components of the mating signal transduction cascade: evidence for STE12 and FAR1.

Molecular Cloning: A Laboratory Manual

2001 • 133,517 citations

A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae.

1989 • 8,705 citations

Transformation of intact yeast cells treated with alkali cations

1983 • 7,688 citations

Rapid and efficient site-specific mutagenesis without phenotypic selection.

1985 • 7,573 citations

A novel genetic system to detect protein–protein interactions

1989 • 6,022 citations

[12] One-step gene disruption in yeast

1983 • 3,302 citations

A ten-minute DNA preparation from yeast efficiently releases autonomous plasmids for transformaion of Escherichia coli

1987 • 2,493 citations

Isolation of monoclonal antibodies specific for human c-myc proto-oncogene product.

1985 • 2,456 citations

A positive selection for mutants lacking orotidine-5′-phosphate decarboxylase activity in yeast: 5-fluoro-orotic acid resistance

1984 • 2,401 citations

Two differentially regulated mRNAs with different 5′ ends encode secreted and intracellular forms of yeast invertase

1982 • 1,539 citations

Transformation in yeast: Development of a hybrid cloning vector and isolation of the can1 gene

1979 • 1,080 citations

Purification of a RAS-responsive adenylyl cyclase complex from Saccharomyces cerevisiae by use of an epitope addition method.

1988 • 983 citations

Isolation of Monoclonal Antibodies Specific for Human c-myc Proto-Oncogene Product

1985 • 788 citations

Mxi1, a protein that specifically interacts with Max to bind Myc-Max recognition sites

1993 • 786 citations

Immunogenic structure of the influenza virus hemagglutinin

1982 • 759 citations

A new class of yeast transcriptional activators

1987 • 706 citations

An Insulin-Stimulated Protein Kinase Similar to Yeast Kinases Involved in Cell Cycle Control

1990 • 701 citations

A eukaryotic transcriptional activator bearing the DNA specificity of a prokaryotic repressor

1985 • 686 citations

Purification, Composition, and Molecular Weight of the β-Galactosidase of Escherichia coli K12

1965 • 660 citations

An essential G1 function for cyclin-like proteins in yeast

1989 • 574 citations

The WHI1+ gene of Saccharomyces cerevisiae tethers cell division to cell size and is a cyclin homolog.

1988 • 563 citations

Extracellular signal-regulated kinases: ERKs in progress.

1991 • 519 citations

G1-specific cyclins of S. cerevisiae: Cell cycle periodicity, regulation by mating pheromone, and association with the p34CDC28 protein kinase

1990 • 449 citations

DAF1, a mutant gene affecting size control, pheromone arrest, and cell cycle kinetics of Saccharomyces cerevisiae.

1988 • 445 citations

Mitogen-activated protein kinases: versatile transducers for cell signaling

1992 • 439 citations

FUS3 encodes a cdc2+/CDC28-related kinase required for the transition from mitosis into conjugation

1990 • 437 citations

The protein kinase homologue Ste20p is required to link the yeast pheromone response G-protein beta gamma subunits to downstream signalling components.

1992 • 427 citations

The Cln3-Cdc28 kinase complex of S. cerevisiae is regulated by proteolysis and phosphorylation.

1992 • 414 citations

Insulin-stimulated microtubule-associated protein kinase is phosphorylated on tyrosine and threonine in vivo.

1988 • 404 citations

Identification of a gene necessary for cell cycle arrest by a negative growth factor of yeast: FAR1 is an inhibitor of a G1 cyclin, CLN2

1990 • 401 citations

The fission yeast cdc2/cdc13/suc1 protein kinase: Regulation of catalytic activity and nuclear localization

1989 • 392 citations

Microtubule-associated protein 2 kinases, ERK1 and ERK2, undergo autophosphorylation on both tyrosine and threonine residues: implications for their mechanism of activation.

1991 • 344 citations

A potential positive feedback loop controlling CLN1 and CLN2 gene expression at the start of the yeast cell cycle

1991 • 341 citations

Purification of a RAS-Responsive Adenylyl Cyclase Complex from Saccharomyces cerevisiae by Use of an Epitope Addition Method

1988 • 328 citations

Requirements for Phosphorylation of Map Kinase During Meiosis in Xenopus Oocytes

1992 • 316 citations

A putative protein kinase overcomes pheromone-induced arrest of cell cycling in S. cerevisiae

1989 • 298 citations

Signal transduction in Saccharomyces cerevisiae requires tyrosine and threonine phosphorylation of FUS3 and KSS1.

1992 • 292 citations

Constitutive mutants of the protein kinase STE11 activate the yeast pheromone response pathway in the absence of the G protein.

1992 • 292 citations

STE12, a protein involved in cell-type-specific transcription and signal transduction in yeast, is part of protein-DNA complexes.

1989 • 274 citations

Xenopus MAP kinase activator: identification and function as a key intermediate in the phosphorylation cascade.

1992 • 264 citations

MAP kinase activator from insulin-stimulated skeletal muscle is a protein threonine/tyrosine kinase.

1992 • 256 citations

Conjugation in Saccharomyces cerevisiae

1988 • 248 citations

The yeast STE12 protein binds to the DNA sequence mediating pheromone induction.

1989 • 245 citations

An Inhibitor of p34 ^CDC28 Protein Kinase Activity from Saccharomyces cerevisiae

1993 • 236 citations

Purification of a murine protein-tyrosine/threonine kinase that phosphorylates and activates the Erk-1 gene product: relationship to the fission yeast byr1 gene product.

1992 • 236 citations

Xenopus MAP kinase activator is a serine/threonine/tyrosine kinase activated by threonine phosphorylation.

1992 • 232 citations

Signal Transduction During Pheromone Response in Yeast

1991 • 225 citations

FUS3 represses CLN1 and CLN2 and in concert with KSS1 promotes signal transduction.

1991 • 211 citations

PHEROMONE RESPONSE IN YEAST

1992 • 204 citations

Recovery of S. cerevisiae a cells from G1 arrest by α factor pheromone requires endopeptidase action

1979 • 193 citations

Nucleotide sequence of the yeast regulatory gene STE7 predicts a protein homologous to protein kinases.

1986 • 189 citations

Order of action of components in the yeast pheromone response pathway revealed with a dominant allele of the STE11 kinase and the multiple phosphorylation of the STE7 kinase.

1992 • 188 citations

STE11 is a protein kinase required for cell-type-specific transcription and signal transduction in yeast.

1990 • 178 citations

Purification and properties of extracellular regulated kinase 1, an insulin-stimulated microtubule-associated protein 2 kinase

1991 • 175 citations

Dual regulation of the yeast CDC28-p40 protein kinase complex: Cell cycle, pheromone, and nutrient limitation effects

1987 • 166 citations

Pheromone-dependent phosphorylation of the yeast STE12 protein correlates with transcriptional activation.

1991 • 148 citations

Autophosphorylation in vitro of recombinant 42-kilodalton mitogen-activated protein kinase on tyrosine.

1991 • 143 citations

Overproduction of the yeast STE12 protein leads to constitutive transcriptional induction.

1990 • 133 citations

DAF1, a Mutant Gene Affecting Size Control, Pheromone Arrest, and Cell Cycle Kinetics of Saccharomyces cerevisiae

1988 • 130 citations

Mouse Erk-1 gene product is a serine/threonine protein kinase that has the potential to phosphorylate tyrosine.

1991 • 113 citations

Phorbol ester stimulates a protein-tyrosine/threonine kinase that phosphorylates and activates the Erk-1 gene product.

1992 • 109 citations

Molecular signal integration. Interplay between serine, threonine, and tyrosine phosphorylation.

1992 • 101 citations

Properties of the DNA-Binding Domain of the Saccharomyces cerevisiae STE12 Protein

1991 • 80 citations

FAR1 is required for posttranscriptional regulation of CLN2 gene expression in response to mating pheromone.

1993 • 66 citations

Phosphorylation of FAR1 in response to alpha-factor: a possible requirement for cell-cycle arrest.

1992 • 65 citations

Functional Redundancy in the Yeast Cell Cycle: FUS3 and KSS1 Have Both Overlapping and Unique Functions

1991 • 63 citations

A novel cyclin gene from Drosophila complements CLN function in yeast.

1991 • 58 citations

Tyrosine phosphorylation of a yeast 40 kDa protein occurs in response to mating pheromone.

1991 • 24 citations

Identification and characterization of a mutation affecting the division arrest signaling of the pheromone response pathway in Saccharomyces cerevisiae.

1990 • 20 citations

FAR1 is Required for Posttranscriptional Regulation of CLN2 Gene Expression in Response to Mating Pheromone

1993 • 16 citations

Deleted Work

1955 • 0 citations