Sst2, a Negative Regulator of Pheromone Signaling in the Yeast Saccharomyces cerevisiae: Expression, Localization, and Genetic Interaction and Physical Association with Gpa1 (the G-Protein α Subunit)

Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

1970 • 253,849 citations

DNA sequencing with chain-terminating inhibitors

1977 • 69,181 citations

Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

1979 • 54,907 citations

Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectors

1985 • 15,222 citations

Antibodies, a laboratory manual

1989 • 12,929 citations

Transformation of intact yeast cells treated with alkali cations

1983 • 7,688 citations

Current protocols in molecular biology

1988 • 7,584 citations

Antibodies: A laboratory manual

1989 • 4,532 citations

The GTPase superfamily: conserved structure and molecular mechanism

1991 • 3,365 citations

Amino Acid Sequences Common to Rapidly Degraded Proteins: The PEST Hypothesis

1986 • 2,566 citations

Isolation of monoclonal antibodies specific for human c-myc proto-oncogene product.

1985 • 2,456 citations

Guide to Yeast Genetics and Molecular Biology

1993 • 2,377 citations

Two differentially regulated mRNAs with different 5′ ends encode secreted and intracellular forms of yeast invertase

1982 • 1,539 citations

High-frequency transformation of yeast: autonomous replication of hybrid DNA molecules.

1979 • 1,504 citations

GTPase inhibiting mutations activate the α chain of Gs and stimulate adenylyl cyclase in human pituitary tumours

1989 • 1,405 citations

Yeast/E. coli shuttle vectors with multiple unique restriction sites

1986 • 1,399 citations

[10] 5-Fluoroorotic acid as a selective agent in yeast molecular genetics

1987 • 1,375 citations

MODEL SYSTEMS FOR THE STUDY OF SEVEN-TRANSMEMBRANE-SEGMENT RECEPTORS

1991 • 1,357 citations

Laboratory course manual for methods in yeast genetics

1986 • 1,310 citations

The neurofibromatosis type 1 gene encodes a protein related to GAP

1990 • 1,099 citations

MAP kinase pathways in yeast: For mating and more

1995 • 1,009 citations

The GAP-related domain of the neurofibromatosis type 1 gene product interacts with ras p21

1990 • 984 citations

The NF1 locus encodes a protein functionally related to mammalian GAP and yeast IRA proteins

1990 • 834 citations

Replacement of chromosome segments with altered DNA sequences constructed in vitro.

1979 • 779 citations

[40] Immunofluorescence methods for yeast

1991 • 705 citations

The catalytic domain of the neurofibromatosis type 1 gene product stimulates ras GTPase and complements ira mutants of S. cerevisiae

1990 • 684 citations

Guide to Yeast Genetics and Molecular Biology

1991 • 607 citations

A GTP-binding protein required for secretion rapidly associates with secretory vesicles and the plasma membrane in yeast

1988 • 592 citations

EGL-10 Regulates G Protein Signaling in the C. elegans Nervous System and Shares a Conserved Domain with Many Mammalian Proteins

1996 • 573 citations

Molecular Cloning of Two Types of GAP Complementary DNA from Human Placenta

1988 • 492 citations

The STE4 and STE18 genes of yeast encode potential β and γ subunits of the mating factor receptor-coupled G protein

1989 • 491 citations

Inhibition of G-protein-mediated MAP kinase activation by a new mammalian gene family

1996 • 470 citations

Molecular mechanisms of membrane receptor desensitization

1993 • 442 citations

The yeast SCG1 gene: A Gα-like protein implicated in the a- and α-factor response pathway

1987 • 431 citations

Phospholipase C-β1 is a GTPase-activating protein for Gq/11, its physiologic regulator

1992 • 430 citations

[31] Tackling the protease problem in Saccharomyces cerevisiae

1991 • 427 citations

Yeast α factor is processed from a larger precursor polypeptide: The essential role of a membrane-bound dipeptidyl aminopeptidase

1983 • 403 citations

Intracellular Targeting and Structural Conservation of a Prohormone-Processing Endoprotease

1989 • 395 citations

Yeast prohormone processing enzyme (KEX2 gene product) is a Ca2+-dependent serine protease.

1989 • 386 citations

Mutants of Saccharomyces cerevisiae unresponsive to cell division control by polypeptide mating hormone.

1980 • 368 citations

GPA1, a haploid-specific essential gene, encodes a yeast homolog of mammalian G protein which may be involved in mating factor signal transduction

1987 • 365 citations

Protein translocation mutants defective in the insertion of integral membrane proteins into the endoplasmic reticulum.

1992 • 352 citations

[33] High-expression vectors with multiple cloning sites for construction of trpE fusion genes: pATH vectors

1991 • 350 citations

S. cerevisiae genes IRA1 and IRA2 encode proteins that may be functionally equivalent to mammalian ras GTPase activating protein

1990 • 333 citations

Purification of Recombinant G Proteins from Sf9 Cells by Hexahistidine Tagging of Associated Subunits

1995 • 332 citations

Regulation of deactivation of photoreceptor G protein by its target enzyme and cGMP

1992 • 324 citations

Isolation and Genetic Analysis of Saccharomyces cerevisiae Mutants Supersensitive to G1 Arrest by a Factor and α Factor Pheromones

1982 • 322 citations

The carboxy-terminal segment of the yeast α-factor receptor is a regulatory domain

1988 • 317 citations

[2] Micromanipulation and dissection of asci

1991 • 303 citations

A putative protein kinase overcomes pheromone-induced arrest of cell cycling in S. cerevisiae

1989 • 298 citations

GAIP, a protein that specifically interacts with the trimeric G protein G alpha i3, is a member of a protein family with a highly conserved core domain.

1995 • 291 citations

[5] Assay of yeast mating reaction

1991 • 286 citations

Control of the yeast bud-site assembly GTPase Cdc42. Catalysis of guanine nucleotide exchange by Cdc24 and stimulation of GTPase activity by Bem3.

1994 • 283 citations

Immunolocalization of Kex2 protease identifies a putative late Golgi compartment in the yeast Saccharomyces cerevisiae.

1991 • 268 citations

Subcellular localization of Cdc42p, a Saccharomyces cerevisiae GTP-binding protein involved in the control of cell polarity.

1993 • 256 citations

Synthesis in Escherichia coli of GTPase-deficient mutants of Gsα

1989 • 247 citations

The proliferation of MAP kinase signaling pathways in yeast

1995 • 243 citations

Amino acid sequence of retinal transducin at the site ADP-ribosylated by cholera toxin.

1984 • 235 citations

Physiological characterization of Saccharomyces cerevisiae mutants supersensitive to G1 arrest by a factor and alpha factor pheromones.

1982 • 216 citations

The C-terminus of the S. cerevisiae α-pheromone receptor mediates an adaptive response to pheromone

1988 • 214 citations

The Saccharomyces cerevisiae BAR1 gene encodes an exported protein with homology to pepsin.

1988 • 212 citations

Pheromonal regulation and sequence of the Saccharomyces cerevisiae SST2 gene: a model for desensitization to pheromone.

1987 • 212 citations

Mutations in the GTP-binding site of GSα alter stimulation of adenylyl cyclase

1989 • 209 citations

Role for the Rho-family GTPase Cdc42 in yeast mating-pheromone signal pathway

1995 • 209 citations

A new family of regulators of G-protein-coupled receptors?

1996 • 209 citations

Overexpression of fIbA, an early regulator of Aspergillus asexual sporulation, leads to activation of brIA and premature initiation of development

1994 • 200 citations

12 Pheromone Response and Signal Transduction during the Mating Process of Saccharomyces cerevisiae

1992 • 200 citations

Interactions between the bud emergence proteins Bem1p and Bem2p and Rho-type GTPases in yeast.

1994 • 199 citations

Inhibition of G-Protein Signaling by Dominant Gain-of-Function Mutations in Sst2p, a Pheromone Desensitization Factor in Saccharomyces cerevisiae

1995 • 198 citations

Recovery of S. cerevisiae a cells from G1 arrest by α factor pheromone requires endopeptidase action

1979 • 193 citations

Pheromone Signalling in Saccharomyces cerevisiae Requires the Small GTP-Binding Protein Cdc42p and Its Activator CDC24

1995 • 192 citations

Regulation of apical transport in epithelial cells by a Gsclass of heterotrimeric G protein

1993 • 188 citations

Isolation and genetic analysis of Saccharomyces cerevisiae mutants supersensitive to G1 arrest by a factor and alpha factor pheromones.

1982 • 178 citations

The STE2 gene product is the ligand-binding component of the alpha-factor receptor of Saccharomyces cerevisiae.

1988 • 174 citations

Separate GTP Binding and GTPase Activating Domains of a Gα Subunit

1993 • 172 citations

THE PHEROMONE RESPONSE PATHWAY IN SACCHAROMYCES CEREVISIAE

1993 • 166 citations

A C-terminal domain of GAP is sufficient to stimulate ras p21 GTPase activity.

1989 • 161 citations

Signal Propagation and Regulation in the Mating Pheromone Response Pathway of the Yeast Saccharomyces cerevisiae

1994 • 157 citations

Degradation of G alpha by the N-end rule pathway

1994 • 154 citations

Clathrin facilitates the internalization of seven transmembrane segment receptors for mating pheromones in yeast.

1993 • 151 citations

Stoichiometry of G protein subunits affects the Saccharomyces cerevisiae mating pheromone signal transduction pathway.

1990 • 146 citations

Overexpression of the STE4 gene leads to mating response in haploid Saccharomyces cerevisiae.

1990 • 145 citations

Constitutive mutants in the yeast pheromone response: Ordered function of the gene products

1989 • 143 citations

Direct evidence for ligand-induced internalization of the yeast alpha-factor pheromone receptor.

1994 • 142 citations

Mutations in a gene encoding the alpha subunit of a Saccharomyces cerevisiae G protein indicate a role in mating pheromone signaling.

1988 • 121 citations

Beta and gamma subunits of a yeast guanine nucleotide-binding protein are not essential for membrane association of the alpha subunit but are required for receptor coupling.

1990 • 118 citations

Mechanism of GTP hydrolysis by G-protein alpha subunits.

1994 • 117 citations

Regulation of the yeast pheromone response pathway by G protein subunits.

1990 • 116 citations

Agonist regulation of cellular G protein levels and distribution: mechanisms and functional implications

1993 • 116 citations

G₁ Cyclin Degradation: the PEST Motif of Yeast Cln2 Is Necessary, but Not Sufficient, for Rapid Protein Turnover

1994 • 108 citations

Yeast cells recover from mating pheromone alpha factor-induced division arrest by desensitization in the absence of alpha factor destruction.

1984 • 98 citations

Determination of the turn-off reaction for the hormone-activated adenylate cyclase.

1979 • 98 citations

Ras membrane targeting is essential for glucose signaling but not for viability in yeast.

1995 • 98 citations

Pheromone-induced phosphorylation of a G protein β subunit in S. cerevisiae is associated with an adaptive response to mating pheromone

1991 • 96 citations

Sec15 protein, an essential component of the exocytotic apparatus, is associated with the plasma membrane and with a soluble 19.5S particle.

1991 • 91 citations

Regulation of Membrane and Subunit Interactions by N-Myristoylation of a G Protein α Subunit in Yeast

1996 • 85 citations

N-myristoylation is required for function of the pheromone-responsive G alpha protein of yeast: conditional activation of the pheromone response by a temperature-sensitive N-myristoyl transferase.

1991 • 83 citations

Cellular variations in heterotrimeric G protein localization and expression in rat pituitary.

1994 • 83 citations

RECEPTOR-G PROTEIN SIGNALING IN YEAST

1991 • 82 citations

Biochemical comparisons of the Saccharomyces cerevisiae Bem2 and Bem3 proteins. Delineation of a limit Cdc42 GTPase-activating protein domain.

1993 • 81 citations

The ras Superfamily of GTPases (1993)

2017 • 77 citations

Mutational activation of the STE5 gene product bypasses the requirement for G protein beta and gamma subunits in the yeast pheromone response pathway.

1994 • 69 citations

Identification of tubulin from the yeast Saccharomyces cerevisiae.

1978 • 63 citations

GPA1Val-50 mutation in the mating-factor signaling pathway in Saccharomyces cerevisiae.

1989 • 61 citations

Disruption of receptor-G protein coupling in yeast promotes the function of an SST2-dependent adaptation pathway.

1993 • 59 citations

Signal transduction and growth control in yeast

1995 • 55 citations

Pheromone action regulates G-protein alpha-subunit myristoylation in the yeast Saccharomyces cerevisiae.

1993 • 52 citations

Genetic Identification of Residues Involved in Association of α and β G-Protein Subunits†

1994 • 51 citations

Mutations in the guanine nucleotide-binding domains of a yeast G alpha protein confer a constitutive or uninducible state to the pheromone response pathway.

1991 • 47 citations

Extracellular suppression allows mating by pheromone-deficient sterile mutants of Saccharomyces cerevisiae

1983 • 47 citations

G protein mutations that alter the pheromone response in Saccharomyces cerevisiae.

1990 • 46 citations

Differential expression of a basic helix-loop-helix phosphoprotein gene, G0S8, in acute leukemia and localization to human chromosome 1q31.

1995 • 42 citations

Regulating G Protein Signaling

1996 • 38 citations

The role of protein structure in the mitochondrial import pathway. Analysis of the soluble F1-ATPase beta-subunit precursor.

1987 • 37 citations

Yeast α-mating factor receptor and G-protein-linked adenylyl cyclase inhibition requires RAS2 and GPA2 activities

1992 • 30 citations

Characterization of the Bar Proteinase, An Extracellular Enzyme from the Yeast Saccharomyces Cerevisiae

1991 • 30 citations

Signal transduction in the budding yeast Saccharomyces cerevisiae

1994 • 29 citations

Role of SGP2, a suppressor of a gpa1 mutation, in the mating-factor signaling pathway of Saccharomyces cerevisiae.

1988 • 28 citations

Cellular variations in heterotrimeric G protein localization and expression in rat pituitary

1994 • 27 citations

Site-directed mutations altering the CAAX box of Ste18, the yeast pheromone-response pathway G gamma subunit.

1994 • 26 citations

Regulation of postreceptor signaling in the pheromone response pathway of Saccharomyces cerevisiae.

1989 • 25 citations

Yeast α‐matching factor receptor‐linked G‐protein signal transduction suppresses Ras‐dependent activity

1991 • 25 citations

Expression of MFα1 in MATa cells supersensitive to α-factor leads to self-arrest

1988 • 23 citations

From Membrane to Nucleus: The Pathway of Signal Transduction in Yeast and Its Genetic Control

1988 • 23 citations

Functional Domains of a Peptide Hormone Receptor: The -Factor Receptor (STE2 Gene Product) of the Yeast Saccharomyces cerevisiae

1988 • 21 citations

Suppressors of a gpa1 mutation cause sterility in Saccharomyces cerevisiae.

1988 • 13 citations

Calendar

1986 • 2 citations