A GTP-binding protein required for secretion rapidly associates with secretory vesicles and the plasma membrane in yeast

Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

1970 • 253,849 citations

“Western Blotting”: Electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A

1981 • 8,657 citations

Methods in yeast genetics

1979 • 7,824 citations

Transformation of intact yeast cells treated with alkali cations

1983 • 7,688 citations

G PROTEINS: TRANSDUCERS OF RECEPTOR-GENERATED SIGNALS

1987 • 6,374 citations

Methods in Yeast Genetics

1988 • 4,905 citations

ras GENES

1987 • 3,818 citations

Intracellular Aspects of the Process of Protein Synthesis

1975 • 3,560 citations

Phase separation of integral membrane proteins in Triton X-114 solution.

1981 • 3,366 citations

Identification of 23 complementation groups required for post-translational events in the yeast secretory pathway

1980 • 1,808 citations

High-frequency transformation of yeast: autonomous replication of hybrid DNA molecules.

1979 • 1,504 citations

Yeast transformation: a model system for the study of recombination.

1981 • 1,491 citations

In yeast, RAS proteins are controlling elements of adenylate cyclase

1985 • 1,142 citations

[17] Isolation of microgram quantities of proteins from polyacrylamide gels for amino acid sequence analysis

1983 • 1,115 citations

Sequences That Regulate the Divergent GAL1-GAL10 Promoter in Saccharomyces cerevisiae

1984 • 955 citations

A ras-like protein is required for a post-Golgi event in yeast secretion

1987 • 894 citations

Order of events in the yeast secretory pathway

1981 • 804 citations

Induction of Membrane Ruffling and Fluid-Phase Pinocytosis in Quiescent Fibroblasts by ras Proteins

1986 • 725 citations

Comparative biochemical properties of normal and activated human ras p21 protein

1984 • 713 citations

The yeast GTP-binding YPT1 protein and a mammalian counterpart are associated with the secretion machinery

1988 • 699 citations

Structure of the GDP Domain of EF-Tu and Location of the Amino Acids Homologous to ras Oncogene Proteins

1985 • 626 citations

The p21 ras C-terminus is required for transformation and membrane association

1984 • 590 citations

Secretion and cell-surface growth are blocked in a temperature-sensitive mutant of Saccharomyces cerevisiae

1979 • 518 citations

Phenotypic analysis of temperature-sensitive yeast actin mutants

1985 • 512 citations

Capacitance measurements reveal stepwise fusion events in degranulating mast cells

1984 • 506 citations

Cloned Viral Protein Vaccine for Foot-and-Mouth Disease: Responses in Cattle and Swine

1981 • 504 citations

Involvement of GTP-binding “G” proteins in transport through the Golgi stack

1987 • 495 citations

Sequences that regulate the divergent GAL1-GAL10 promoter in Saccharomyces cerevisiae.

1984 • 462 citations

Antibodies to the Golgi complex and the rough endoplasmic reticulum.

1982 • 418 citations

Localization of the src gene product of the Harvey strain of MSV to plasma membrane of transformed cells by electron microscopic immunocytochemistry

1980 • 404 citations

Harvey murine sarcoma virus p21 ras protein: biological and biochemical significance of the cysteine nearest the carboxy terminus.

1984 • 403 citations

A yeast gene encoding a protein homologous to the human c-has/bas proto-oncogene product

1983 • 396 citations

The transforming proteins of Rous sarcoma virus, Harvey sarcoma virus and Abelson virus contain tightly bound lipid

1982 • 357 citations

Analysis of adenovirus transforming proteins from early regions 1A and 1B with antisera to inducible fusion antigens produced in Escherichia coli

1984 • 349 citations

Two roles for guanine nucleotides in the stimulus-secretion sequence of neutrophils

1986 • 339 citations

Cytochrome c Oxidase from Bakers' Yeast

1973 • 331 citations

Mutant ras-encoded proteins with altered nucleotide binding exert dominant biological effects.

1986 • 285 citations

The ras-related YPT1 gene product in yeast: A GTP-binding protein that might be involved in microtubule organization

1986 • 281 citations

RAM, a gene of yeast required for a functional modification of RAS proteins and for production of mating pheromone a-factor

1986 • 259 citations

A Model for the Tertiary Structure of p21, the Product of the ras Oncogene

1985 • 248 citations

Guanine nucleotides induce Ca2+-independent insulin secretion from permeabilized RINm5F cells.

1987 • 244 citations

The effects of vanadate on the plasma membrane ATPase of Neurospora crassa.

1979 • 231 citations

SELECTIVE RELEASE OF CONTENT FROM MICROSOMAL VESICLES WITHOUT MEMBRANE DISASSEMBLY

1973 • 220 citations

Induction of Galactokinase in Saccharomyces cerevisiae : Kinetics of Induction and Glucose Effects

1972 • 203 citations

Two classes of fatty acid acylated proteins exist in eukaryotic cells.

1985 • 196 citations

Cloning of Saccharomyces cerevisiae DNA replication genes: isolation of the CDC8 gene and two genes that compensate for the cdc8-1 mutation.

1983 • 192 citations

Essential synergy between Ca2+ and guanine nucleotides in exocytotic secretion from permeabilized rat mast cells.

1987 • 188 citations

Purification and characterization of constitutive secretory vesicles from yeast.

1987 • 179 citations

Localized secretion of acid phosphatase reflects the pattern of cell surface growth in saccharomyces cerevisiae

1980 • 175 citations

Direct identification of palmitic acid as the lipid attached to p21ras.

1986 • 157 citations

Specific binding of [alpha-32P]GTP to cytosolic and membrane-bound proteins of human platelets correlates with the activation of phospholipase C.

1987 • 157 citations

Identification of a precursor in the biosynthesis of the p21 transforming protein of harvey murine sarcoma virus

1982 • 151 citations

Characterization of the plasma membrane Mg2+-ATPase from the yeast, Saccharomyces cerevisiae.

1979 • 144 citations

Yeast secretory mutants that block the formation of active cell surface enzymes.

1984 • 131 citations

The Oncogenic Activation of Human p21 ^ras by a Novel Mechanism

1986 • 130 citations

Fatty acid acylation of eucaryotic cell membrane proteins

1982 • 128 citations

Characterization of new mutants in the early part of the yeast secretory pathway isolated by a [3H]mannose suicide selection.

1987 • 127 citations

Posttranslational processing of p21 ras proteins involves palmitylation of the C-terminal tetrapeptide containing cysteine-186

1985 • 120 citations

Export of major cell surface proteins is blocked in yeast secretory mutants

1983 • 117 citations

Processing and fatty acid acylation of RAS1 and RAS2 proteins in Saccharomyces cerevisiae.

1986 • 108 citations

Fatty acylation is important but not essential for Saccharomyces cerevisiae RAS function.

1987 • 98 citations

Calcium shares the limelight in stimulus-secretion coupling

1986 • 97 citations

Polyacrylamide-agarose beads for the preparation of effective immunoabsorbents

1976 • 93 citations

A novel yeast mutant defective in the processing of ras proteins: assessment of the effect of the mutation on processing steps.

1987 • 93 citations

Studies on the Microsomal Electron-transport System of Anaerobically Grown Yeast

1977 • 92 citations

Endocytosis in Saccharomyces cerevisiae: internalization of enveloped viruses into spheroplasts.

1985 • 39 citations

Sec53, a protein required for an early step in secretory protein processing and transport in yeast, interacts with the cytoplasmic surface of the endoplasmic reticulum.

1987 • 30 citations

Immunocytochemical localization in normal and transformed human cells in tissue culture using a monoclonal antibody to the src protein of the Harvey strain of Murine Sarcoma Virus

1983 • 28 citations