Selective induction of cyclo‐oxygenase‐2 activity in the permanent human endothelial cell line HUV‐EC‐C: biochemical and pharmacological characterization

Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

1970 • 253,849 citations

Single-Step Method of RNA Isolation by Acid Guanidinium Thiocyanate–Phenol–Chloroform Extraction

1987 • 64,270 citations

Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

1979 • 54,907 citations

Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction

1987 • 45,673 citations

Lounging in a lysosome: the intracellular lifestyle of Coxiella burnetii

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Inhibition of Prostaglandin Synthesis as a Mechanism of Action for Aspirin-like Drugs

1971 • 8,596 citations

TIS10, a phorbol ester tumor promoter-inducible mRNA from Swiss 3T3 cells, encodes a novel prostaglandin synthase/cyclooxygenase homologue

1991 • 1,814 citations

Pentoxifylline

1987 • 1,768 citations

Selectivity of nonsteroidal antiinflammatory drugs as inhibitors of constitutive and inducible cyclooxygenase.

1993 • 1,655 citations

Pharmacological and biochemical demonstration of the role of cyclooxygenase 2 in inflammation and pain.

1994 • 1,579 citations

Human cyclooxygenase-2 cDNA.

1992 • 1,493 citations

Drugs which inhibit prostaglandin biosynthesis.

1974 • 1,325 citations

Selective inhibition of inducible cyclooxygenase 2 in vivo is antiinflammatory and nonulcerogenic.

1994 • 1,322 citations

Differential inhibition of prostaglandin endoperoxide synthase (cyclooxygenase) isozymes by aspirin and other non-steroidal anti-inflammatory drugs.

1993 • 1,270 citations

Risks of bleeding peptic ulcer associated with individual non-steroidal anti-inflammatory drugs

1994 • 881 citations

New insights into the mode of action of anti-inflammatory drugs

1995 • 853 citations

cDNA cloning and functional activity of a glucocorticoid-regulated inflammatory cyclooxygenase.

1992 • 838 citations

NS-398, a new anti-inflammatory agent, selectively inhibits prostaglandin G/H synthase/cyclooxygenase (COX-2) activity in vitro

1994 • 826 citations

Selective expression of mitogen-inducible cyclooxygenase in macrophages stimulated with lipopolysaccharide.

1992 • 823 citations

Molecular cloning of human prostaglandin endoperoxide synthase type II and demonstration of expression in response to cytokines.

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Expression of mRNA for cyclooxygenase‐1 and cyclooxygenase‐2 in human tissues

1993 • 708 citations

Prostaglandin endoperoxide synthase: regulation of enzyme expression

1991 • 642 citations

Human platelet/erythroleukemia cell prostaglandin G/H synthase: cDNA cloning, expression, and gene chromosomal assignment

1991 • 580 citations

Prostanoid biosynthesis and mechanisms of action

1992 • 551 citations

Biochemical and pharmacological characterization of the cyclooxygenase activity of human blood prostaglandin endoperoxide synthases.

1994 • 500 citations

Structure of the mitogen-inducible TIS10 gene and demonstration that the TIS10-encoded protein is a functional prostaglandin G/H synthase.

1992 • 498 citations

Cyclooxygenase is an immediate-early gene induced by interleukin-1 in human endothelial cells.

1990 • 484 citations

Endogenous glucocorticoids regulate an inducible cyclooxygenase enzyme.

1992 • 471 citations

Prostaglandin and thromboxane biosynthesis

1991 • 444 citations

Expression and selective inhibition of the constitutive and inducible forms of human cyclo-oxygenase

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Dexamethasone inhibits mitogen induction of the TIS10 prostaglandin synthase/cyclooxygenase gene.

1992 • 412 citations

Mechanism of selective inhibition of the inducible isoform of prostaglandin G/H synthase.

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1994 • 366 citations

Purification, characterization and selective inhibition of human prostaglandin G/H synthase 1 and 2 expressed in the baculovirus system

1994 • 339 citations

Cloning of human gene encoding prostaglandin endoperoxide synthase and primary structure of the enzyme

1989 • 333 citations

Pharmacology of a selective cyclooxygenase-2 inhibitor, L-745,337: a novel nonsteroidal anti-inflammatory agent with an ulcerogenic sparing effect in rat and nonhuman primate stomach.

1995 • 312 citations

Purification and characterization of a novel, distinct isoform of prostaglandin endoperoxide synthase induced by human chorionic gonadotropin in granulosa cells of rat preovulatory follicles.

1992 • 312 citations

Fatty Acid Substrate Specificities of Human Prostaglandin-endoperoxide H Synthase-1 and −2

1995 • 294 citations

Demonstration of an inducible cyclooxygenase in human endothelial cells using antibodies raised against the carboxyl-terminal region of the cyclooxygenase-2.

1993 • 293 citations

On the mechanism of prostacyclin and thromboxane A2 biosynthesis

1989 • 287 citations

Oxygenation of Unsaturated Fatty Acids by the Vesicular Gland of Sheep

1967 • 279 citations

Hormonal regulation of messenger ribonucleic acid encoding a novel isoform of prostaglandin endoperoxide H synthase in rat preovulatory follicles. Induction in vivo and in vitro.

1992 • 260 citations

Constitutive cyclooxygenase (COX-1) and inducible cyclooxygenase (COX-2): Rationale for selective inhibition and progress to date

1996 • 248 citations

Serum and Glucocorticoid Regulation of Gene Transcription and Expression of the Prostaglandin H Synthase-1 and Prostaglandin H Synthase-2 Isozymes

1993 • 224 citations

Comparison of Hydroperoxide Initiator Requirements for the Cyclooxygenase Activities of Prostaglandin H Synthase-1 and −2

1995 • 219 citations

Lipopolysaccharide priming of alveolar macrophages for enhanced synthesis of prostanoids involves induction of a novel prostaglandin H synthase.

1992 • 212 citations

Enzymatic conversion of prostaglandin endoperoxide H2 and arachidonic acid to prostacyclin by cultured human endothelial cells.

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Isolation and characterization of thromboxane synthase from human platelets as a cytochrome P-450 enzyme.

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Selective inhibition of cyclooxygenase 2

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Expression of the protein product of the prostaglandin synthase-2/TIS10 gene in mitogen-stimulated Swiss 3T3 cells.

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Effect of inhibitor time-dependency on selectivity towards cyclooxygenase isoforms

1995 • 156 citations

Nimesulide

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Stimulation of prostaglandin formation by vasoactive mediators in cultured human endothelial cells

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Regulation of prostaglandin endoperoxide synthase (cyclooxygenase) isozyme expression

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Lipopolysaccharide induces prostaglandin H synthase-2 in alveolar macrophages

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A Review of Gastric Ulcer and Gastroduodenal Injury in Normal Volunteers Receiving Aspirin and Other Non-Steroidal Anti-Inflammatory Drugs

1989 • 123 citations

Interaction between peroxidase and cyclooxygenase activities in prostaglandin-endoperoxide synthase. Interpretation of reaction kinetics.

1994 • 122 citations

The Catalytic Outcomes of the Constitutive and the Mitogen Inducible Isoforms of Prostaglandin H2 Synthase Are Markedly Affected by Glutathione and Glutathione Peroxidase(s)

1995 • 119 citations

Biosynthesis and metabolism of 15-hydroperoxy-5,8,11,13-eicosatetraenoic acid by human umbilical vein endothelial cells.

1984 • 117 citations

Prostaglandin D, E, and F synthases

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On the Organization and Mechanism of Prostaglandin Synthetase

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Formation of 9-Hydroxyoctadecadienoic Acid from Linoleic Acid in Endothelial Cells

1989 • 89 citations

IL-1 and related cytokines enhance thrombin-stimulated PGI2 production in cultured endothelial cells without affecting thrombin-stimulated von Willebrand factor secretion or platelet-activating factor biosynthesis.

1989 • 83 citations

Effects of the novel anti‐inflammatory compounds, N‐[2‐(cyclohexyloxy)‐4‐nitrophenyl] methanesulphonamide (NS‐398) and 5‐methanesulphonamido‐6‐(2, 4‐difluorothiophenyl)‐1‐indanone (L‐745, 337), on the cyclo‐oxygenase activity of human blood prostaglandin endoperoxide synthases

1995 • 83 citations

Transcriptional regulation of endothelial constitutive PGHS-1 expression by phorbol ester

1996 • 82 citations

Induction of cyclooxygenase ii in human synovial microvessel endothelial cells by interleukin‐1

1994 • 72 citations

Prostacyclin and thromboxane synthases

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Identification of novel arachidonic acid metabolites formed by prostaglandin H synthase

1987 • 62 citations

Interleukin-1 Enhances the Ability of Cultured Human Umbilical Vein Endothelial Cells to Oxidize Linoleic Acid

1995 • 59 citations

Epidermal Cell—Polymorphonuclear Leukocyte Cooperation in the Formation of Leukotriene B4 by Transcellular Biosynthesis

1992 • 54 citations

Metabolism of [1-14C] — arachidonic acid by cultured calf aortic endothelial cells: Evidence for the presence of a lipoxygenase pathway

1985 • 52 citations

Effect of NS-398, a new nonsteroidal anti-inflammatory agent, on gastric ulceration and acid secretion in rats.

1993 • 52 citations

Chemical and enzymic conversions of the prostaglandin endoperoxide PGH2.

1980 • 46 citations

Intimate Relation between Cyclooxygenase and Peroxidase Activities of Prostaglandin H Synthase. Peroxidase Reaction of Ferulic Acid and Its Influence on the Reaction of Arachidonic Acid

1994 • 33 citations

Profile of prostaglandins induced by endothelin-1 in human brain capillary endothelium

1993 • 33 citations

Interleukin-1 increases 15-hydroxyeicosatetraenoic acid formation in cultured human endothelial cells

1993 • 32 citations

Analysis and Quantitation of Splicing Variants of the TPA-Inducible PGHS-1 mRNA in Rat Tracheal Epithelial Cells

1995 • 26 citations

Responses of Human Dermal Microvascular Endothelial Cells to Histamine and Their Modulation by Interleukin 1 and Substance P

1991 • 18 citations

The enzymic oxidation of 6-methylpurine

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Gastrointestinal damage demonstrated with nabumetone or etodolac in preclinical studies

1993 • 9 citations