cDNA cloning and functional activity of a glucocorticoid-regulated inflammatory cyclooxygenase.

Single-Step Method of RNA Isolation by Acid Guanidinium Thiocyanate–Phenol–Chloroform Extraction

1987 • 64,270 citations

A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity

1983 • 26,063 citations

Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease

1979 • 22,482 citations

Purification of Biologically Active Globin Messenger RNA by Chromatography on Oligothymidylic acid-Cellulose

1972 • 6,922 citations

High-efficiency transformation of mammalian cells by plasmid DNA.

1987 • 5,377 citations

Unidirectional digestion with exonuclease III creates targeted breakpoints for DNA sequencing

1984 • 4,231 citations

A conserved AU sequence from the 3′ untranslated region of GM-CSF mRNA mediates selective mRNA degradation

1986 • 4,200 citations

Patterns of Amino Acids near Signal‐Sequence Cleavage Sites

1983 • 2,290 citations

TIS10, a phorbol ester tumor promoter-inducible mRNA from Swiss 3T3 cells, encodes a novel prostaglandin synthase/cyclooxygenase homologue

1991 • 1,814 citations

In situ detection of mycoplasma contamination in cell cultures by fluorescent Hoechst 33258 stain

1977 • 1,743 citations

Expression of a mitogen-responsive gene encoding prostaglandin synthase is regulated by mRNA splicing.

1991 • 1,734 citations

Identification of a common nucleotide sequence in the 3'-untranslated region of mRNA molecules specifying inflammatory mediators.

1986 • 1,591 citations

The induction and suppression of prostaglandin H2 synthase (cyclooxygenase) in human monocytes.

1990 • 940 citations

Primary structure of prostaglandin G/H synthase from sheep vesicular gland determined from the complementary DNA sequence.

1988 • 604 citations

A serum- and glucocorticoid-regulated 4-kilobase mRNA encodes a cyclooxygenase-related protein.

1991 • 571 citations

Cyclooxygenase is an immediate-early gene induced by interleukin-1 in human endothelial cells.

1990 • 484 citations

Purification of the cyclooxygenase that forms prostaglandins. Demonstration of two forms of iron in the holoenzyme.

1976 • 435 citations

Isolation and characterization of the complementary DNA for sheep seminal vesicle prostaglandin endoperoxide synthase (cyclooxygenase).

1988 • 431 citations

The aspirin and heme-binding sites of ovine and murine prostaglandin endoperoxide synthases.

1990 • 429 citations

The CTAB-DNA precipitation method: a common mini-scale preparation of template DNA from phagemids, phages or plasmids suitable for sequencing.

1989 • 414 citations

Selective regulation of cellular cyclooxygenase by dexamethasone and endotoxin in mice.

1990 • 404 citations

Regulation of fibroblast cyclooxygenase synthesis by interleukin-1.

1988 • 401 citations

Mouse cells transformed by bovine papillomavirus contain only extrachromosomal viral DNA sequences.

1981 • 387 citations

Temporal and pharmacological division of fibroblast cyclooxygenase expression into transcriptional and translational phases.

1989 • 235 citations

The AU-rich sequences in the 3' untranslated region mediate the increased turnover of interferon mRNA induced by glucocorticoids.

1991 • 170 citations

Aspirin inhibits interleukin 1-induced prostaglandin H synthase expression in cultured endothelial cells.

1991 • 140 citations

Corticosteroids suppress cyclooxygenase messenger RNA levels and prostanoid synthesis in cultured vascular cells

1988 • 119 citations

Identification of sequences within the murine granulocyte-macrophage colony-stimulating factor mRNA 3'-untranslated region that mediate mRNA stabilization induced by mitogen treatment of EL-4 thymoma cells.

1991 • 87 citations

Plasmids for the cloning and expresion of full-length double-stranded cDNAs under control of the SV40 early or late gene promoter

1983 • 84 citations

Persistent induction of cyclooxygenase in p60v-src-transformed 3T3 fibroblasts.

1990 • 82 citations

Eicosanoid forming enzyme mRNA in human tissues. Analysis by quantitative polymerase chain reaction

1991 • 79 citations

Influence of natural and recombinant interleukin 2 on endothelial cell arachidonate metabolism. Induction of de novo synthesis of prostaglandin H synthase.

1988 • 71 citations

Posttranscriptional Regulation of Surfactant Protein-A Messenger RNA in Human Fetal Lungin Vitroby Glucocorticoids

1991 • 68 citations

[15] Very-high-resolution two-dimensional electrophoretic separation of proteins on giant gels

1983 • 68 citations

Effect of in Vitro Virus Infection on Response of Human Monocytes and Lymphocytes to Mitogen Stimulation

1978 • 65 citations

Isolation of the cDNA for human prostaglandin H synthase

1986 • 55 citations

Glucocorticoid-induced reduction of prostanoid synthesis in TPA-differentiated U937 cells is mainly due to a reduced cyclooxygenase activity

1990 • 41 citations

Growth factor- and dexamethasone-induced proteins in Swiss 3T3 cells. Relationship to DNA synthesis.

1985 • 24 citations

Structure-function relationships in sheep, mouse, and human prostaglandin endoperoxide G/H synthases.

1990 • 23 citations

Regulation of prostanoids synthesis in human fibroblasts and human blood monocytes by interleukin-1, endotoxin, and glucocorticoids.

1990 • 16 citations

Bovine papillomavirus type 1 alters the processing of host glucose- and calcium-modulated endoplasmic reticulum proteins

1991 • 5 citations