The diversity of antigen‐specific monoclonal antibodies from transgenic mice bearing human immunoglobulin gene miniloci

[1] Preparation of monoclonal antibodies: Strategies and procedures

1981 • 1,787 citations

A B cell-deficient mouse by targeted disruption of the membrane exon of the immunoglobulin μ chain gene

1991 • 1,783 citations

By-passing immunization

1991 • 1,603 citations

Reshaping human antibodies for therapy

1988 • 1,555 citations

Replacing the complementarity-determining regions in a human antibody with those from a mouse

1986 • 1,356 citations

Chimeric human antibody molecules: mouse antigen-binding domains with human constant region domains.

1984 • 1,002 citations

Production of functional chimaeric mouse/human antibody

1984 • 539 citations

Surface Antigen Expression and Immunoglobulin Gene Rearrangement During Mouse pre‐B Cell Development

1983 • 490 citations

Sequence homologies, N sequence insertion and JH gene utilization in VHDJH joining: implications for the joining mechanism and the ontogenetic timing of Ly1 B cell and B-CLL progenitor generation.

1990 • 396 citations

Elements regulating somatic hypermutation of an immunoglobulin κ gene: Critical role for the intron enhancer/matrix attachment region

1994 • 382 citations

Antigen-specific human antibodies from mice comprising four distinct genetic modifications

1994 • 382 citations

Antigen–specific human monoclonal antibodies from mice engineered with human Ig heavy and light chain YACs

1994 • 351 citations

Organization of human immunoglobulin heavy chain diversity gene loci.

1988 • 317 citations

mRNA sequences define an unusually restricted IgG response to 2-phenyloxazolone and its early diversification

1983 • 301 citations

A hapten-specific chimaeric IgE antibody with human physiological effector function

1985 • 289 citations

Kinetic maturation of an immune response

1991 • 274 citations

Discriminating intrinsic and actigen-selected mutational hotspots in immunoglobulin V genes

1993 • 222 citations

Human immunoglobulin D segments encoded in tandem multigenic families

1981 • 219 citations

Deletion and editing of B cells that express antibodies to DNA.

1994 • 173 citations

The use of chromosomal translocations to study human immunoglobulin gene organization: mapping DH segments within 35 kb of the C mu gene and identification of a new DH locus.

1988 • 155 citations

Engineering Antibodies for Therapy

1992 • 151 citations

A repertoire of monoclonal antibodies with human heavy chains from transgenic mice.

1989 • 145 citations

Analysis of somatic hypermutation in mouse Peyer's patches using immunoglobulin kappa light-chain transgenes.

1993 • 108 citations

Sliding distance between actin and myosin filaments per ATP molecule hydrolysed in skinned muscle fibres

1991 • 105 citations

Transgenic mice containing a human heavy chain immunoglobulin gene fragment cloned in a yeast artificial chromosome

1993 • 87 citations

Human antibody production in transgenic mice: expression from 100 kb of the human IgH locus

1991 • 82 citations

A transgenic mouse that expresses a diversity of human sequence heavy and light chain immunoglobulins

1992 • 82 citations

Human immunoglobulin transgenes undergo rearrangement, somatic mutation and class switching in mice that lack endogenous IgM

1994 • 74 citations

Creation of Mice Expressing Human Antibody Light Chains by Introduction of a Yeast Artificial Chromosome Containing the Core Region of the Human Immunoglobulin κ Locus

1993 • 45 citations

Serological and genetic characterization of a human monoclonal immunoglobulin G anti-DNA idiotype.

1994 • 44 citations

Human immunoglobulin heavy-chain minilocus recombination in transgenic mice: gene-segment use in mu and gamma transcripts.

1993 • 34 citations

Antibodies generated from human immunoglobulin miniloci in transgenic mice

1994 • 32 citations

A single-step procedure for cloning and selection of antibody-secreting hybridomas

1990 • 24 citations

Biased utilization of DHQ52 and JH4 gene segments in a human Ig transgenic minilocus is independent of antigenic selection.

1994 • 13 citations

[10] Optimal strategies for developing human—human monoclonal antibodies

1986 • 10 citations

Generation of antibody repertoires in transgenic mice

1991 • 7 citations