Active unfolding of precursor proteins during mitochondrial protein import

Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid

1978 • 4,472 citations

Proteins: Structures and Molecular Properties

1986 • 2,694 citations

Speculations on the functions of the major heat shock and glucose-regulated proteins

1986 • 1,574 citations

A subfamily of stress proteins facilitates translocation of secretory and mitochondrial precursor polypeptides

1988 • 1,504 citations

Protein Folding Intermediates: Native-State Hydrogen Exchange

1995 • 980 citations

Requirement for hsp70 in the mitochondrial matrix for translocation and folding of precursor proteins

1990 • 756 citations

Binding of a specific ligand inhibits import of a purified precursor protein into mitochondria

1986 • 723 citations

Phenotypic expression in E. coli of a DNA sequence coding for mouse dihydrofolate reductase

1978 • 426 citations

What drives the translocation of proteins?

1992 • 415 citations

Mitochondrial Hsp70/MIM44 complex facilitates protein import

1994 • 391 citations

[14] Isolation of highly purified mitochondria from Saccharomyces cerevisiae

1995 • 308 citations

[26] A T5 promoter-based transcription-translation system for the analysis of proteins in Vitro and in Vivo

1987 • 274 citations

Can Hsp70 proteins act as force-generating motors?

1995 • 270 citations

The Role of Hsp70 in Conferring Unidirectionality on Protein Translocation into Mitochondria

1994 • 269 citations

A novel in vitro transcription-translation system: accurate and efficient synthesis of single proteins from cloned DNA sequences.

1984 • 251 citations

The Escherichia coli DnaK chaperone, the 70-kDa heat shock protein eukaryotic equivalent, changes conformation upon ATP hydrolysis, thus triggering its dissociation from a bound target protein

1991 • 245 citations

A precursor protein partly translocated into yeast mitochondria is bound to a 70 kd mitochondrial stress protein.

1990 • 242 citations

Mitochondrial protein import: biochemical and genetic evidence for interaction of matrix hsp70 and the inner membrane protein MIM44.

1994 • 238 citations

The amino-terminal region of an imported mitochondrial precursor polypeptide can direct cytoplasmic dihydrofolate reductase into the mitochondrial matrix.

1984 • 236 citations

Barnase and barstar: two small proteins to fold and fit together

1989 • 222 citations

Sequential action of mitochondrial chaperones in protein import into the matrix.

1991 • 221 citations

Mitochondrial molecular chaperones: their role in protein translocation

1994 • 203 citations

A 70-kd protein of the yeast mitochondrial outer membrane is targeted and anchored via its extreme amino terminus.

1984 • 198 citations

Complex interactions between the chaperonin 60 molecular chaperone and dihydrofolate reductase

1991 • 190 citations

Dynamic interaction between Isp45 and mitochondrial hsp70 in the protein import system of the yeast mitochondrial inner membrane.

1994 • 188 citations

Imported mitochondrial proteins cytochrome b2 and cytochrome c1 are processed in two steps.

1982 • 187 citations

A mitochondrial homolog of bacterial GrpE interacts with mitochondrial hsp70 and is essential for viability.

1994 • 182 citations

Unfolding and refolding of a purified precursor protein during import into isolated mitochondria.

1988 • 182 citations

Structure, expression and regulation of a nuclear gene encoding a mitochondrial protein: the yeast L(+)-lactate cytochrome c oxidoreductase (cytochrome b2).

1985 • 178 citations

Guanidinium Chloride Induction of Partial Unfolding in Amide Proton Exchange in RNase A

1993 • 176 citations

Phosphodiester bond cleavage outside mitochondria is required for the completion of protein import into the mitochondrial matrix

1987 • 175 citations

Polypeptides traverse the mitochondrial envelope in an extended state

1990 • 172 citations

Mitochondrial protein import: Reversible binding of the presequence at the trans side of the outer membrane drives partial translocation and unfolding

1995 • 171 citations

A role for a eukaryotic GrpE-related protein, Mge1p, in protein translocation.

1994 • 167 citations

Presequence and mature part of preproteins strongly influence the dependence of mitochondrial protein import on heat shock protein 70 in the matrix.

1993 • 158 citations

A mitochondrial import factor purified from rat liver cytosol is an ATP-dependent conformational modulator for precursor proteins.

1993 • 157 citations

Point mutations destabilizing a precursor protein enhance its post-translational import into mitochondria.

1988 • 128 citations

Movement of the position of the transition state in protein folding

1995 • 126 citations

Latent membrane perturbation activity of a mitochondrial precursor protein is exposed by unfolding.

1988 • 120 citations

Import of cytochrome b₂ to the mitochondrial intermembrane space: The tightly folded heme‐binding domain makes import dependent upon matrix ATP

1993 • 116 citations

Refolding of Barnase in the Presence of GroE

1993 • 108 citations

Disrupted yeast mitochondria can import precursor proteins directly through their inner membrane.

1989 • 108 citations

Protein import into the yeast mitochondrial matrix. A new translocation intermediate between the two mitochondrial membranes.

1991 • 107 citations

The Yeast Mitochondrial Protein Import Receptor Mas20p Binds Precursor Proteins through Electrostatic Interaction with the Positively Charged Presequence

1995 • 105 citations

Precursor proteins in transit through mitochondrial contact sites interact with hsp70 in the matrix

1990 • 98 citations

A Mutation in the Yeast Heat-Shock Factor Gene Causes Temperature-Sensitive Defects in Both Mitochondrial Protein Import and the Cell Cycle

1991 • 94 citations

Protein Sorting: Pulling in the proteins

1995 • 94 citations

Characterization of the mitochondrial processing peptidase of Neurospora crassa.

1994 • 93 citations

Precursor binding to yeast mitochondria. A general role for the outer membrane protein Mas70p.

1993 • 91 citations

Mitochondrial GrpE Is Present in a Complex with hsp70 and Preproteins in Transit across Membranes

1994 • 91 citations

Hsp60-independent protein folding in the matrix of yeast mitochondria.

1996 • 91 citations

Solution small-angle x-ray scattering study of the molecular chaperone Hsc70 and its subfragments

1995 • 90 citations

The signal that sorts yeast cytochrome b2 to the mitochondrial intermembrane space contains three distinct functional regions.

1993 • 85 citations

Extrapolation to water of kinetic and equilibrium data for the unfolding of barnase in urea solutions

1994 • 78 citations

Conformational Characterization of DnaK and Its Complexes by Small-Angle X-ray Scattering

1996 • 72 citations

More models of muscle movement

1994 • 71 citations

Mitochondrial GrpE is present in a complex with hsp70 and preproteins in transit across membranes.

1994 • 69 citations

Expression of Bacillus amyloliquefaciens extracellular ribonuclease (barnase) in Escherichia coli following an inactivating mutation

1987 • 67 citations

A matrix ATP requirement for presequence translocation across the inner membrane of mitochondria.

1993 • 60 citations

The sorting signal of cytochrome b2 promotes early divergence from the general mitochondrial import pathway and restricts the unfoldase activity of matrix Hsp70.

1995 • 57 citations

Dihydrofolate reductase

1986 • 56 citations

A mutation in the yeast heat-shock factor gene causes temperature-sensitive defects in both mitochondrial protein import and the cell cycle.

1991 • 48 citations

Precursor of mitochondrial aspartate aminotransferase synthesized in Escherichia coli is complexed with heat‐shock protein DnaK

1992 • 33 citations

Chapter 17 Import of Precursor Proteins into Yeast Submitochondrial Particles

1991 • 14 citations

Analysis of Protein Folding by Protein Engineering

1994 • 7 citations

Phenotypic expression in E. coli of a DNA sequence coding for mouse dihydrofolate reductase. 1978.

1992 • 4 citations