Detection of human cytokines in situ using antibody and probe based methods

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2001 • 133,517 citations

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1981 • 14,396 citations

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1989 • 12,929 citations

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1986 • 3,477 citations

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1968 • 2,480 citations

The use of avidin-biotin interaction in immunoenzymatic techniques.

1979 • 1,734 citations

Lymphokine Production by Human T Cells in Disease States

1994 • 1,351 citations

Interleukin-4, -5, and -6 and tumor necrosis factor-alpha in normal and asthmatic airways: evidence for the human mast cell as a source of these cytokines.

1994 • 850 citations

Interleukin 6 is expressed in high levels in psoriatic skin and stimulates proliferation of cultured human keratinocytes.

1989 • 844 citations

Expression of mRNA for interleukin-5 in mucosal bronchial biopsies from asthma.

1991 • 841 citations

Messenger RNA expression of the cytokine gene cluster, interleukin 3 (IL-3), IL-4, IL-5, and granulocyte/macrophage colony-stimulating factor, in allergen-induced late-phase cutaneous reactions in atopic subjects.

1991 • 676 citations

Quantitative analysis of cytokine gene expression in rheumatoid arthritis.

1990 • 610 citations

Cytokine messenger RNA expression for IL-3, IL-4, IL-5, and granulocyte/macrophage-colony-stimulating factor in the nasal mucosa after local allergen provocation: relationship to tissue eosinophilia.

1992 • 572 citations

Cytokine expression in the brain during the acquired immunodeficiency syndrome

1992 • 572 citations

Immunolocalization of cytokines in the nasal mucosa of normal and perennial rhinitic subjects. The mast cell as a source of IL-4, IL-5, and IL-6 in human allergic mucosal inflammation.

1993 • 561 citations

AIDS Kaposi sarcoma-derived cells produce and respond to interleukin 6.

1990 • 500 citations

Influence of grass pollen immunotherapy on cellular infiltration and cytokine mRNA expression during allergen-induced late-phase cutaneous responses.

1993 • 479 citations

Production of cytokines around loosened cemented acetabular components. Analysis with immunohistochemical techniques and in situ hybridization.

1993 • 439 citations

Cellular localization of interleukin-8 and its inducer, tumor necrosis factor-alpha in psoriasis.

1991 • 437 citations

Acute phase response in exercise. III. Neutrophil and IL-1 beta accumulation in skeletal muscle

1993 • 413 citations

Alkaline phosphatase and peroxidase for double immunoenzymatic labelling of cellular constituents.

1978 • 408 citations

Inhibition of interleukin 8 attenuates angiogenesis in bronchogenic carcinoma.

1994 • 373 citations

Increases in Activated T Lymphocytes, Eosinophils, and Cytokine mRNA Expression for Interleukin-5 and Granulocyte/Macrophage Colony-stimulating Factor in Bronchial Biopsies after Allergen Inhalation Challenge in Atopic Asthmatics

1993 • 336 citations

Detection of interleukin-6 and alpha 2-macroglobulin immunoreactivity in cortex and hippocampus of Alzheimer's disease patients.

1992 • 329 citations

Nonradioactive labeling of oligonucleotides in vitro with the hapten digoxigenin by tailing with terminal transferase

1991 • 322 citations

Local production of tumor necrosis factor and IFN-gamma in tuberculous pleuritis.

1990 • 318 citations

Interleukin 5 synthesis by eosinophils: association with granules and immunoglobulin-dependent secretion.

1994 • 295 citations

Expression of interleukin-8 gene in inflammatory bowel disease is related to the histological grade of active inflammation.

1994 • 282 citations

In situ production of TNF-α, IL-1β and IL-2R in ANCA-positive glomerulonephritis

1993 • 278 citations

Induction of tumor necrosis factor alpha production by human hepatocytes in chronic viral hepatitis.

1994 • 267 citations

Tumor necrosis factor and its receptors in human ovarian cancer. Potential role in disease progression.

1993 • 254 citations

In vivo CD40-gp39 interactions are essential for thymus-dependent humoral immunity. I. In vivo expression of CD40 ligand, cytokines, and antibody production delineates sites of cognate T-B cell interactions.

1993 • 250 citations

Characterization of Inflammatory Infiltrates in Chronic Chagasic Myocardial Lesions: Presence of Tumor Necrosis Factor-α+ Cells and Dominance of Granzyme A+, CD8+ Lymphocytes

1993 • 250 citations

Enhanced production of the chemotactic cytokines interleukin-8 and monocyte chemoattractant protein-1 in human abdominal aortic aneurysms.

1993 • 237 citations

Production of interleukins in human immunodeficiency virus-1-replicating lymph nodes.

1990 • 237 citations

DETECTION OF CYTOKINES AT THE CARTILAGE/PANNUS JUNCTION IN PATIENTS WITH RHEUMATOID ARTHRITIS: IMPLICATIONS FOR THE ROLE OF CYTOKINES IN CARTILAGE DESTRUCTION AND REPAIR

1992 • 233 citations

Interleukin-6 gene expression in Castleman's disease

1991 • 231 citations

Localization of interleukin-1 type I receptor and interleukin-1 beta in human endometrium throughout the menstrual cycle.

1993 • 225 citations

TNFα mRNA expression in allergic inflammation

1991 • 199 citations

Preferential messenger RNA expression of Th1-type cells (IFN-gamma+, IL-2+) in classical delayed-type (tuberculin) hypersensitivity reactions in human skin.

1992 • 192 citations

Immunohistological detection of interleukin 1-like molecules and tumour necrosis factor in human epidermis before and after UVB-irradiation in vivo

1988 • 190 citations

Production of tumor necrosis factor and interleukin-1 by macrophages from human atheromatous plaques.

1993 • 186 citations

A comparison of two immunoperoxidase staining methods based on the avidin-biotin interaction.

1984 • 175 citations

Upper Keratinocytes of Psoriatic Skin Lesions Express High Levels of NAP-1/IL-8 mRNA In Situ

1991 • 175 citations

Increased IL-6 Production by Monocytes and Keratinocytes in Patients with Psoriasis

1991 • 162 citations

Tumor Necrosis Factor-α Messenger Ribonucleic Acid and Protein in Human Endometrium1

1992 • 159 citations

Detection and localization of cytokine immunoreactivity in retro‐ocular connective tissue in Graves' opthalmopathy

1993 • 149 citations

IL-1 activity is reduced in psoriatic skin. Decreased IL-1 alpha and increased nonfunctional IL-1 beta.

1990 • 140 citations

Detection of mRNAs for macrophage products in inflammatory bowel disease by in situ hybridisation.

1992 • 138 citations

Concomitant in vivo production of 19 different cytokines in human tonsils.

1994 • 138 citations

Detection of inflammatory cytokine messenger RNA (mRNA)-expressing cells in human inflamed gingiva by combined in situ hybridization and immunohistochemistry.

1992 • 138 citations

In vitro and in vivo interleukin-8 production in human renal cortical epithelia

1992 • 138 citations

Expression of interleukin-6 and interleukin-6 receptor in Hodgkin's disease

1991 • 133 citations

In situ expression of cytokines in IgA nephritis

1993 • 133 citations

Cytokine Expression in the Spinal Cord Lesions in HTLV-l-associated Myelopathy

1994 • 131 citations

Interleukin-6, IL-6 receptor, and IL-6 nuclear factor gene expression in paget's disease

1994 • 131 citations

Interleukin-6 production in high-grade B lymphomas: correlation with the presence of malignant immunoblasts in acquired immunodeficiency syndrome and in human immunodeficiency virus-seronegative patients

1992 • 119 citations

Production of interleukin-10 by human bronchogenic carcinoma.

1994 • 119 citations

PRODUCTION OF CYTOKINES (TNF-ALPHA, IL-1-BETA) AND ENDOTHELIAL CELL ACTIVATION IN HUMAN LIVER ALLOGRAFT REJECTION1

1993 • 115 citations

Evidence for local and systemic activation of immune cells by peritumoral injections of interleukin 2 in patients with advanced squamous cell carcinoma of the head and neck.

1993 • 112 citations

In vivo production of interleukin‐10 by malignant cells in AIDS lymphomas

1992 • 112 citations

Topical glucocorticosteroid (fluticasone propionate) inhibits cells expressing cytokine mRNA for interleukin-4 in the nasal mucosa in allergen-induced rhinitis.

1994 • 110 citations

Distribution of interleukin-6 in normal and diseased human kidney.

1991 • 110 citations

The cytokine facts book

1995 • 109 citations

Cellular localization of tumor necrosis factor (TNF)-alpha transcripts in normal bowel and in necrotizing enterocolitis. TNF gene expression by Paneth cells, intestinal eosinophils, and macrophages.

1993 • 106 citations

Lymphotoxin, tumour necrosis factor and interleukin‐6 gene transcripts are present in Hodgkin and Reed‐Sternberg cells of most Hodgkin's disease cases

1993 • 105 citations

Human Placenta Constitutively Produces Interleukin-8 during Pregnancy and Enhances its Production in Intrauterine Infection1

1992 • 104 citations

IN SITU EXPRESSION OF TUMOR NECROSIS FACTOR-ALPHA, INTERFERON-GAMMA, AND INTERLEUKIN-2 RECEPTORS IN RENAL ALLOGRAFT BIOPSIES

1992 • 104 citations

Analysis of naturally occurring delayed-type hypersensitivity reactions in leprosy by in situ hybridization.

1989 • 103 citations

DIFFERENTIAL IN SITU EXPRESSION OF CYTOKINES IN RENAL ALLOGRAFT REJECTION

1991 • 102 citations

Interleukin-8 Immunoreactivity in the Skin of Healthy Subjects and Patients with Palmoplantar Pustulosis and Psoriasis

1992 • 101 citations

In vivo cytokine profiles in allergic and irritant contact dermatitis

1995 • 100 citations

Human Keratinocyte Locomotion: The Effect of Selected Cytokines

1992 • 99 citations

The interleukin 2 gene is expressed in the syncytiotrophoblast of the human placenta.

1989 • 98 citations

Cytokine expression in T-cell lymphomas and Hodgkin's disease. Its possible implication in autocrine or paracrine production as a potential basis for neoplastic growth.

1991 • 98 citations

IL-6 production by human T lymphocytes. Expression in HTLV-1-infected but not in normal T cells.

1991 • 96 citations

The digoxigenin:anti-digoxigenin (DIG) technology—a survey on the concept and realization of a novel bioanalytical indicator system

1991 • 96 citations

T lymphocytes and mast cells express messenger RNA for interleukin-4 in the nasal mucosa in allergen-induced rhinitis.

1994 • 94 citations

Interleukin-6 production in high-grade B lymphomas: correlation with the presence of malignant immunoblasts in acquired immunodeficiency syndrome and in human immunodeficiency virus-seronegative patients

1992 • 92 citations

Patterns of cytokine gene expression in infectious mononucleosis

1994 • 88 citations

Interleukin-1 and Interleukin-6 in Psoriasis

1990 • 84 citations

In situ hybridization of IL-6 in rheumatoid arthritis

1992 • 82 citations

Expression of mRNA for cytokines in tumor‐infiltrating mononuclear cells in ovarian adenocarcinoma and invasive breast cancer

1992 • 79 citations

Multiple immunoenzyme staining techniques use of fluoresceinated, biotinylated and unlabelled monoclonal antibodies

1989 • 78 citations

Immunopathology and cytokine detection in the skin lesions of patients with kawasaki disease

1993 • 77 citations

Increased expression of interleukin 6 mRNA in cardiac myxomas.

1993 • 76 citations

Immunologic responsiveness in American cutaneous leishmaniasis lesions.

1990 • 76 citations

EVIDENCE FOR A NONCLASSICAL PATHWAY OF GRAFT REJECTION INVOLVING INTERLEUKIN 5 AND EOSINOPHILS

1993 • 73 citations

Immunohistologic assessment of cytokine production of infiltrating cells in various forms of leprosy.

1990 • 73 citations

Expression of interleukin-1 and interleukin-1 receptor antagonist by human rheumatoid synovial tissue macrophages

1992 • 72 citations

Localization and Characterization of the Interleukin 1 Immunoreactive Pool (IL-1 alpha and beta Forms) in Normal Human Epidermis

1989 • 72 citations

Investigation of cytokine gene expression in human colorectal cancer.

1990 • 71 citations

Tumor necrosis factor-alpha and IFN-gamma expression in human thymus. Localization and overexpression in Down syndrome (trisomy 21).

1992 • 70 citations

In situ hybridization of interleukin-1 in CD14-positive cells in rheumatoid arthritis

1992 • 70 citations

Distribution of interleukin 1 receptor antagonist protein (IRAP), interleukin 1 receptor, and interleukin 1α in normal and psoriatic skin. Decreased expression of IRAP in psoriatic lesional epidermis

1992 • 68 citations

In situ expression of messenger RNA of interleukin-1 and interleukin-6 in psoriasis: interleukin-6 involved in formation of psoriatic lesions

1991 • 67 citations

Immunological functions and in vivo cell-cell interactions of T cells in the spleen.

1992 • 65 citations

Localization of interleukin-1 type I receptor and interleukin-1 beta in human endometrium throughout the menstrual cycle

1993 • 64 citations

Interleukin-6 in normal skin and psoriasis.

1992 • 63 citations

Detection of interleukin-6 and interleukin-1 production in human thyroid epithelial cells by non-radioactivein situhybridization and immunohistochemical methods

1991 • 62 citations

Ubiquitous Expression of TNF‐α/Cachectin Immunoreactivity in Human Endometrium

1991 • 60 citations

Immunohistochemistry II

1994 • 60 citations

Detection of endogenous cytokines in sera or in lymph nodes obtained from patients with sarcoidosis

1991 • 57 citations

IL-2 mRNA expression in Tac-positive malignant lymphomas.

1990 • 56 citations

Tumor necrosis factor expression by human ovarian carcinoma in vivo.

1991 • 56 citations

In situ production of interleukins in hyperplastic thymus from myasthenia gravis patients

1991 • 53 citations

Production of cytokines in sarcoid lymph nodes: Preferential expression of interleukin-1β and interferon-γ genes

1992 • 51 citations

Ependymal cells of the choroid plexus express tumour necrosis factor‐α¹

1993 • 51 citations

Tumor necrosis factor alpha and lymphotoxin production in Hodgkin's disease.

1990 • 49 citations

The role of cytokines in the generation of skin lesions in dermatitis herpetiformis

1993 • 48 citations

Tumor necrosis factor production during human renal allograft rejection is associated with depression of plasma protein C and free protein S levels and decreased intragraft thrombomodulin expression.

1992 • 48 citations

Production of matrix metalloproteinases 2 and 3 (stromelysin) by stromal cells of giant cell tumor of bone.

1992 • 47 citations

What's New in “in Situ Hybridization”

1987 • 46 citations

Recruitment of T lymphocytes and induction of tumor necrosis factor in thyroid cancer by a local immunotherapy

1992 • 45 citations

Interleukin-6 and TNFα production in human renal cell carcinoma

1993 • 44 citations

TGFβ-1 and TNFα Expression in the Epidermis of Patients with Epidermodysplasia Verruciformis

1991 • 44 citations

Expression of IL-6 mRNA in normal rat and human pituitaries and in human pituitary adenomas.

1994 • 44 citations

Tumor necrosis factor-α release during systemic reaction in cold urticaria

1994 • 43 citations

Detection of in vivo production of tumour necrosis factor-alpha by human thyroid epithelial cells.

1992 • 41 citations

Epidermal Interleukin 1α Functional Activity and Interleukin 8 Immunoreactivity Are Increased in Patients with Cutaneous T-Cell Lymphoma

1991 • 41 citations

Immunohistochemical detection of co-localizing cytokine and antibody producing cells in the extrafollicular area of human palatine tonsils

1993 • 41 citations

Immunoreactivity for IL-1 beta and TNF alpha in human lymphoid and nonlymphoid tissues.

1989 • 40 citations

Se´zary syndrome with elevated serum IgE and hypereosinophilia: Role of dysregulated cytokine production

1993 • 39 citations

Keratinocyte-expression of interleukin-6 but not of tumour necrosis factor-alpha is increased in the allergic and the irritant patch test reaction.

1991 • 39 citations

Enhanced Expression of Resident Leukocyte Interferon γ mRNA in Endometriosis

1993 • 39 citations

Tumor Necrosis Factor and IL-1β Expression in Pediatric Patients with Inflammatory Bowel Disease

1993 • 38 citations

In vivo kinetics and characterization of IFN-gamma-producing cells during a thymus-independent immune response.

1991 • 37 citations

Cytokines in health and disease

1993 • 36 citations

Immunohistochemical demonstration of interleukin-1 receptor antagonist protein and interleukin-1 in human lymphoid tissue and granulomas.

1992 • 36 citations

Local production of interleukin-6 during acute rejection in human renal allografts

1993 • 36 citations

Membrane and Cytosolic Interleukin-1 Alpha and Beta in Normal Human Epidermal Cells: Variability of Epitope Exposure in Immunohistochemistry

1990 • 36 citations

Immunohistochemical studies on NAP-1/IL-8 in contact eczema and atopic dermatitis

1992 • 33 citations

Interleukin-2 and Interferon-gamma Production in Follicular Lymphomas

1991 • 32 citations

In situ hybridization techniques for the analysis of gene expression: Applications in tumor pathology

1994 • 32 citations

In vivo immunohistochemical identification of tumor necrosis factor/cachectin in human lymphoid tissue.

1989 • 32 citations

Localization of interleukin 6 mRNA in human tonsils by in situ hybridization

1989 • 31 citations

in situ expression of the IL‐I‐α and tnf‐α genes by reed‐sternberg cells in Hodgkin's disease

1992 • 29 citations

Double immunocytochemical staining for the in situ study of allotype distribution during an anti-trinitrophenyl immune response in chimeric rabbits.

1986 • 29 citations

Genes for Interleukin-1, Interleukin-6, and Tumor Necrosis Factor are Expressed at Markedly Reduced Levels in the Livers of Patients with Severe Liver Disease

1991 • 28 citations

Tumor necrosis factor-alpha in the retina in acquired immune deficiency syndrome.

1992 • 27 citations

Cytokine expression and endothelial cell and lymphocyte activation in human cardiac allograft rejection: an immunohistochemical study of endomyocardial biopsy samples.

1992 • 26 citations

New advances on cytokines

1993 • 25 citations

Sequential double immunocytochemical staining for in situ identification of an auto-anti-allotype immune response in allotype-suppressed rabbits.

1988 • 25 citations

In situ Localization of lnterleukin-6 in Normal Skin and Atrophic Cutaneous Disease

1992 • 21 citations

An investigation of T-cell subset phenotype and function in the rheumatoid synovium using in situ hybridization for IL-2 mRNA.

1991 • 19 citations

Detection and localization of HIV RNA and TNF mRNA in rectal biopsies from patients with AIDS

1993 • 18 citations

Monokine gene expression in normal human liver: selective involvement of the portal compartment

1992 • 18 citations

Detection of IL-2 at mRNA and protein levels in synovial infiltrates from inflammatory arthropathies using biotinylated oligonucleotide probes in situ

1991 • 17 citations

In situ hybridization of the mRNA for interferon-γ, interferon-αE, interferon-β, interleukin-1β and interleukin-6 and characterization of infiltrating cells in thyroid tissues

1992 • 16 citations

Phenotypic characterization of individual interferon‐γ‐producing cells after OKT3 antibody activation

1986 • 14 citations

Non-isotopic in situ detection of mRNA for interleukin-4 in archival human tissue

1994 • 14 citations

The distribution of immunoreactive interferon-gamma-containing cells in normal human tissues.

1992 • 11 citations

Fixation of cryo-sections under HIV-1 inactivating conditions: integrity of antigen binding sites and cell surface antigens

1991 • 11 citations

Differential in situ expression of cytokine genes in human renal transplantation.

1993 • 10 citations

In situ expression of cytokines and serine esterase B in small‐bowel allograft rejection

1993 • 10 citations

IL-1 mRNA expression detected by in situ hybridization and polymerase chain reaction in human thymus and thymoma. Correlation with late T-cell maturation in normal versus neoplastic thymus.

1993 • 4 citations

Immunohistochemistry in the analysis of lymphoid organs and their function

1996 • 4 citations

Immunohisto- chemistry in the analysis of lymphoid organs and their function

1996 • 1 citations