Immobilized pH gradient two‐dimensional gel electrophoresis and mass spectrometric identification of cytokine‐regulated proteins in ME‐180 cervical carcinoma cells

An approach to correlate tandem mass spectral data of peptides with amino acid sequences in a protein database

1994 • 6,412 citations

Rapid identification of proteins by peptide-mass fingerprinting

1993 • 1,619 citations

Error-Tolerant Identification of Peptides in Sequence Databases by Peptide Sequence Tags

1994 • 1,475 citations

Identifying proteins from two-dimensional gels by molecular mass searching of peptide fragments in protein sequence databases.

1993 • 1,301 citations

In-gel digestion of proteins for internal sequence analysis after one- or two-dimensional gel electrophoresis

1992 • 1,259 citations

Isoelectric focusing in immobilized pH gradients: Principle, methodology and some applications

1982 • 963 citations

Use of mass spectrometric molecular weight information to identify proteins in sequence databases

1993 • 936 citations

Catalysis of guanine nucleotide exchange on Ran by the mitotic regulator RCC1

1991 • 703 citations

Peptide Mass Maps: A Highly Informative Approach to Protein Identification

1993 • 678 citations

Protein Identification by Mass Profile Fingerprinting

1993 • 595 citations

A giant nucleopore protein that binds Ran/TC4

1995 • 492 citations

Metabolic acidosis stimulates muscle protein degradation by activating the adenosine triphosphate-dependent pathway involving ubiquitin and proteasomes.

1994 • 394 citations

Sample application by in‐gel rehydration improves the resolution of two‐dimensional electrophoresis with immobilized pH gradients in the first dimension

1994 • 367 citations

Functions of the Proteasome: the Lysis at the End of the Tunnel

1995 • 302 citations

Characterization of Four Novel ras-Like Genes Expressed in a Human Teratocarcinoma Cell Line

1990 • 285 citations

Mass spectrometric sequencing of linear peptides by product‐ion analysis in a reflectron time‐of‐flight mass spectrometer using matrix‐assisted laser desorption ionization

1993 • 269 citations

Techniques in Protein Chemistry V

1993 • 268 citations

Characterization of proteins that interact with the cell-cycle regulatory protein Ran/TC4

1993 • 261 citations

Selective protein degradation: A journey's end within the proteasome

1995 • 259 citations

Human RanGTPase-activating protein RanGAP1 is a homologue of yeast Rna1p involved in mRNA processing and transport.

1995 • 247 citations

Rapid mass spectrometric peptide sequencing and mass matching for characterization of human melanoma proteins isolated by two-dimensional PAGE.

1995 • 206 citations

Characterization of four novel ras-like genes expressed in a human teratocarcinoma cell line.

1990 • 202 citations

Identification of MECL-1 (LMP-10) as the third IFN-gamma-inducible proteasome subunit.

1996 • 196 citations

Micropreparative two‐dimensional electrophoresis allowing the separation of samples containing milligram amounts of proteins

1993 • 191 citations

cDNA Cloning and Interferon γ Down-Regulation of Proteasomal Aubunits X and Y

1994 • 181 citations

A third interferon‐γ‐induced subunit exchange in the 20S proteasome

1996 • 168 citations

Proteasome components with reciprocal expression to that of the MHC-encoded LMP proteins

1994 • 157 citations

Interlaboratory reproducibility of yeast protein patterns analyzed by immobilized pH gradient two‐dimensional gel electrophoresis

1995 • 152 citations

Mass Spectrometry in the Biological Sciences

1996 • 148 citations

Human RanGTPase activating protein RanGAP1 is a homolog of yeast RNA1p involved in messenger RNA processing and transport

1995 • 125 citations

The human keratinocyte two‐dimensional gel protein database (update 1992): Towards an integrated approach to the study of cell proliferation, differentiation and skin diseases

1992 • 125 citations

Imidazole‐SDS‐Zn reverse staining of proteins in gels containing or not SDS and microsequence of individual unmodified electroblotted proteins

1992 • 107 citations

Cytokines : a practical approach

1995 • 101 citations

A family of Ran binding proteins that includes nucleoporins.

1995 • 79 citations

Highly resolving two-dimensional gels for protein sequencing.

1991 • 79 citations

Epitope mapping of the gastrin‐releasing peptide/anti‐bombesin monoclonal antibody complex by proteolysis followed by matrix‐assisted laser desorption ionization mass spectrometry

1994 • 79 citations

Mass spectrometric and Edman sequencing of lipocortin I isolated by two-dimensional SDS/PAGE of human melanoma lysates.

1993 • 78 citations

Effects of mutant Ran/TC4 proteins on cell cycle progression.

1994 • 77 citations

Modulation of the growth of transformed cells by human tumor necrosis factor-alpha and interferon-gamma.

1987 • 71 citations

Separate Domains of the Ran GTPase Interact with Different Factors To Regulate Nuclear Protein Import and RNA Processing

1995 • 68 citations

Horizontal two‐dimensional electrophoresis with immobilized pH gradients using PhastSystem

1988 • 67 citations

The human keratinocyte two‐dimensional protein database (update 1994): Towards an integrated approach to the study of cell proliferation, differentiation and skin diseases

1994 • 54 citations

A Permanent Zn2+ Reverse Staining Method for the Detection and Quantification of Proteins in Polyacrylamide Gels

1993 • 47 citations

Micropreparative immobilized pH gradient two‐dimensional electrophoresis in combination with protein microsequencing for the analysis of human liver proteins

1995 • 42 citations

Role of protein phosphorylation in TNF‐induced apoptosis: Phosphatase inhibitors synergize with TNF to activate DNA fragmentation in normal as well as TNF‐resistant U937 variants

1993 • 38 citations

Identification of cytokine‐regulated proteins in normal and malignant cells by the combination of two‐dimensional polyacrylamide gel electrophoresis, mass spectrometry, Edman degradation and immunoblotting and approaches to the analysis of their functional roles

1996 • 25 citations

Synergistic induction of polypeptides by tumor necrosis factor and interferon‐gamma in cells sensitive or resistant to tumor necrosis factor: Assessment by computer based analysis of two‐dimensional gels using the PDQUEST system

1990 • 21 citations

Isolation and Characterization of α-Type HC3 and β-Type HC5 Subunit Genes of Human Proteasomes

1994 • 19 citations

Enhanced synthesis of tumor necrosis factor-inducible proteins, plasminogen activator inhibitor-2, manganese superoxide dismutase, and protein 28/5.6, is selectively triggered by the 55-kDa tumor necrosis factor receptor in human melanoma cells

1994 • 16 citations

The Involvement of Protein Tyrosine Kinase Activity in a Tumor Necrosis Factor Resistance Mechanism

1995 • 12 citations

Differing patterns of human protooncogene expression in peripheral blood and bone marrow acute leukemia cells.

1987 • 10 citations

Mass Spectrometric Identification of Proteins Isolated By Two-Dimensional Gel Electrophoresis

1996 • 8 citations

Two-dimensional gel electrophoresis for purification, sequencing, and identification of cytokine-induced proteins in normal and malignant cells

1995 • 6 citations