Sample application by in‐gel rehydration improves the resolution of two‐dimensional electrophoresis with immobilized pH gradients in the first dimension

Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa

1987 • 11,491 citations

Sequence from picomole quantities of proteins electroblotted onto polyvinylidene difluoride membranes.

1987 • 5,066 citations

Two‐dimensional electrophoresis. The current state of two‐dimensional electrophoresis with immobilized pH gradients

1988 • 2,431 citations

In-gel digestion of proteins for internal sequence analysis after one- or two-dimensional gel electrophoresis

1992 • 1,259 citations

The focusing positions of polypeptides in immobilized pH gradients can be predicted from their amino acid sequences

1993 • 969 citations

Methods for increasing the resolution of two-dimensional protein electrophoresis

1988 • 650 citations

Quantitation of microgram amounts of protein in two‐dimensional polyacrylamide gel electrophoresis sample buffer

1985 • 489 citations

Immobilized pH Gradients: Theory and Methodology

1990 • 363 citations

Elimination of point streaking on silver stained two‐dimensional gels by addition of iodoacetamide to the equilibration buffer

1987 • 271 citations

A comparison between low background silver diammine and silver nitrate protein stains

1992 • 266 citations

Micropreparative two‐dimensional electrophoresis allowing the separation of samples containing milligram amounts of proteins

1993 • 191 citations

A new multiphasic buffer system for sodium dodecyl sulfate‐polyacrylamide gel electrophoresis of proteins and peptides with molecular masses 100 000–1000, and their detection with picomolar sensitivity

1991 • 96 citations

Polymerization kinetics of polyacrylamide gels containing immobilized ph gradients for isoelectric focusing

1984 • 79 citations

Mass spectrometric and Edman sequencing of lipocortin I isolated by two-dimensional SDS/PAGE of human melanoma lysates.

1993 • 78 citations

Human liver protein map: Update 1993

1993 • 75 citations

Of matrices and men

1989 • 67 citations

Horizontal two‐dimensional electrophoresis with immobilized pH gradients in the first dimension in the presence of nonionic detergent

1987 • 59 citations

Immobilized pH gradients: Effect of salts, added carrier ampholytes and voltage gradients on protein patterns

1988 • 56 citations

Fractionation techniques in a hydro-organic environment

1984 • 44 citations

Tris-tricine and Tris-borate buffer systems provide better estimates of human mesothelial cell intermediate filament protein molecular weights than the standard Tris-glycine system

1991 • 42 citations

Effect of various detergents on protein migration in the second dimension of two-dimensional gels

1991 • 36 citations

The Immobiline family: From “vacuum” to “plenum” chemistry

1992 • 29 citations

Two‐dimensional electrophoresis with immobilized pH gradients in the first dimension: Protein focusing as a function of time

1987 • 24 citations

HydroLinkTM gel electrophoresis (HLGE). I. Matrix characterization

1989 • 24 citations

A universal method for two-dimensional polyacrylamide gel electrophoresis of membrane proteins using isoelectric focusing on immobilized pH gradients in the first dimension

1991 • 22 citations

A reliable two‐dimensional gel electrophoresis procedure for separating neural proteins

1991 • 17 citations

Immobilized pH 2.5–11 gradients for two‐dimensional electrophoresis

1992 • 15 citations

Factors affecting the range of pH gradients in the isoelectric focusing dimennsion of two‐dimensional gel electrophoresis: The effects of reservoir electrolytes and loading procedures

1985 • 13 citations