Molecular Determinants of Metalloproteinase Substrate Specificity: Matrix Metalloproteinase Substrate Binding Domains, Modules, and Exosites

Why genes in pieces?

1978 • 2,256 citations

Matrix Metalloproteinases and Other Matrix Proteinases in Relation to Cariology: The Era of ‘Dentin Degradomics'

2015 • 2,211 citations

Mechanism Of Cell Surface Activation Of 72-kDa Type IV Collagenase

1995 • 1,576 citations

Localization of Matrix Metalloproteinase MMP-2 to the Surface of Invasive Cells by Interaction with Integrin αvβ3

1996 • 1,555 citations

A novel metalloproteinase gene specifically expressed in stromal cells of breast carcinomas

1990 • 1,140 citations

SV40-transformed Human Lung Fibroblasts Secrete a 92-kDa Type IV Collagenase Which Is Identical to That Secreted by Normal Human Macrophages

1989 • 1,122 citations

Signal transduction through the fibronectin receptor induces collagenase and stromelysin gene expression.

1989 • 1,073 citations

H-ras oncogene-transformed human bronchial epithelial cells (TBE-1) secrete a single metalloprotease capable of degrading basement membrane collagen.

1988 • 1,050 citations

Membrane Type 1 Matrix Metalloproteinase Digests Interstitial Collagens and Other Extracellular Matrix Macromolecules

1997 • 926 citations

Matrix Metalloproteinase-2 Is an Interstitial Collagenase

1995 • 917 citations

Tissue Inhibitor of Metalloproteinase (TIMP-2)

1989 • 899 citations

[13] Evolutionary families of metallopeptidases

1995 • 794 citations

Inflammation Dampened by Gelatinase A Cleavage of Monocyte Chemoattractant Protein-3

2000 • 771 citations

Matrix metalloproteinases: structures, evolution, and diversification

1998 • 759 citations

Cellular Mechanisms for Human Procollagenase-3 (MMP-13) Activation

1996 • 741 citations

Astacins, serralysins, snake venom and matrix metalloproteinases exhibit identical zinc‐binding environments (HEXXHXXGXXH and Met‐turn) and topologies and should be grouped into a common family, the ‘metzincins’

1993 • 729 citations

Multiple modes of activation of latent human fibroblast collagenase: evidence for the role of a Cys73 active-site zinc complex in latency and a "cysteine switch" mechanism for activation.

1990 • 699 citations

Independent Regulation of Collagenase, 72-kDa Progelatinase, and Metalloendoproteinase Inhibitor Expression in Human Fibroblasts by Transforming Growth Factor-β

1989 • 649 citations

Matrix Metalloproteinase Activity Inactivates the CXC Chemokine Stromal Cell-derived Factor-1

2001 • 649 citations

2.0 Å Crystal Structure of a Four-Domain Segment of Human Fibronectin Encompassing the RGD Loop and Synergy Region

1996 • 621 citations

Furin-dependent intracellular activation of the human stromelysin-3 zymogen

1995 • 620 citations

The TIMP2 Membrane Type 1 Metalloproteinase “Receptor” Regulates the Concentration and Efficient Activation of Progelatinase A

1998 • 578 citations

The Soluble Catalytic Domain of Membrane Type 1 Matrix Metalloproteinase Cleaves the Propeptide of Progelatinase A and Initiates Autoproteolytic Activation

1996 • 564 citations

Transcriptional and post-transcriptional regulation of 72-kDa gelatinase/type IV collagenase by transforming growth factor-beta 1 in human fibroblasts. Comparisons with collagenase and tissue inhibitor of matrix metalloproteinase gene expression

1991 • 461 citations

Transmembrane-deletion Mutants of the Membrane-type Matrix Metalloproteinase-1 Process Progelatinase A and Express Intrinsic Matrix-degrading Activity

1996 • 392 citations

The collagenase gene family in humans consists of at least four members

1988 • 364 citations

Three-dimensional Type I Collagen Lattices Induce Coordinate Expression of Matrix Metalloproteinases MT1-MMP and MMP-2 in Microvascular Endothelial Cells

1998 • 358 citations

The Role of the C-terminal Domain of Human Collagenase-3 (MMP-13) in the Activation of Procollagenase-3, Substrate Specificity, and Tissue Inhibitor of Metalloproteinase Interaction

1997 • 345 citations

The X-ray crystal structure of the catalytic domain of human neutrophil collagenase inhibited by a substrate analogue reveals the essentials for catalysis and specificity.

1994 • 342 citations

Structural properties of matrix metalloproteinases

1999 • 324 citations

Structure and distribution of modules in extracellular proteins

1996 • 322 citations

Structure of the Catalytic Domain of Fibroblast Collagenase Complexed with an Inhibitor

1994 • 321 citations

The role of the C-terminal domain in collagenase and stromelysin specificity.

1992 • 311 citations

Contribution of hydrogen bonding to the conformational stability of ribonuclease T1

1992 • 273 citations

Binding of gelatinases A and B to type-I collagen and other matrix components

1995 • 270 citations

The C-terminal domain of 72 kDa gelatinase A is not required for catalysis, but is essential for membrane activation and modulates interactions with tissue inhibitors of metalloproteinases

1992 • 268 citations

Structure of full-length porcine synovial collagenase reveals a C-terminal domain containing a calcium-linked, four-bladed β-propeller

1995 • 260 citations

Identification and Characterization of a Novel Human Matrix Metalloproteinase with Unique Structural Characteristics, Chromosomal Location, and Tissue Distribution

1997 • 254 citations

The activity of the tissue inhibitors of metalloproteinases is regulated by C-terminal domain interactions: A kinetic analysis of the inhibition of gelatinase A

1993 • 237 citations

Concanavalin A produces a matrix-degradative phenotype in human fibroblasts. Induction and endogenous activation of collagenase, 72-kDa gelatinase, and Pump-1 is accompanied by the suppression of the tissue inhibitor of matrix metalloproteinases.

1990 • 235 citations

How Big Is the Universe of Exons?

1990 • 228 citations

Cloning and Characterization of Human MMP-23, a New Matrix Metalloproteinase Predominantly Expressed in Reproductive Tissues and Lacking Conserved Domains in Other Family Members

1999 • 226 citations

Proteolytic processing of membrane-type-1 matrix metalloproteinase is associated with gelatinase A activation at the cell surface

1998 • 223 citations

Extracellular Matrix Binding Properties of Recombinant Fibronectin Type II-like Modules of Human 72-kDa Gelatinase/Type IV Collagenase

1995 • 221 citations

The Structural Basis for the Elastolytic Activity of the 92-kDa and 72-kDa Gelatinases

1996 • 216 citations

Assessment of the role of the fibronectin-like domain of gelatinase A by analysis of a deletion mutant.

1994 • 211 citations

Fragments of human fibroblast collagenase. Purification and characterization

1989 • 210 citations

Structure of the catalytic domain of human fibroblast collagenase complexed with an inhibitor

1994 • 197 citations

Regulation of Membrane‐Type Matrix Metalloproteinase‐1 Expression by Growth Factors and Phorbol 12‐Myristate 13‐Acetate

1996 • 184 citations

Implication of collagen type I-induced membrane-type 1-matrix metalloproteinase expression and matrix metalloproteinase-2 activation in the metastatic progression of breast carcinoma.

1997 • 177 citations

Comparative sequence specificities of human 72- and 92-kDa gelatinases (type IV collagenases) and PUMP (matrilysin)

1993 • 172 citations

Structure of human neutrophil collagenase reveals large S1′ specificity pocket

1994 • 163 citations

Specific, High Affinity Binding of Tissue Inhibitor of Metalloproteinases-4 (TIMP-4) to the COOH-terminal Hemopexin-like Domain of Human Gelatinase A

1997 • 160 citations

Identification of Cysteine 354 of β-Tubulin as Part of the Binding Site for the A Ring of Colchicine

1996 • 160 citations

Analysis of the role of the COOH-terminal domain in the activation, proteolytic activity, and tissue inhibitor of metalloproteinase interactions of gelatinase B.

1994 • 158 citations

Membrane associated matrix metalloproteinases in metastasis

1999 • 147 citations

The 72 kDa type IV collagenase is modulated via differential expression of alpha v beta 3 and alpha 5 beta 1 integrins during human melanoma cell invasion.

1993 • 147 citations

Sequence specificities of human fibroblast and neutrophil collagenases.

1991 • 146 citations

Alanine scanning mutagenesis and functional analysis of the fibronectin-like collagen-binding domain from human 92-kDa type IV collagenase.

1992 • 146 citations

Tissue inhibitor of metalloproteinases-4 inhibits but does not support the activation of gelatinase A via efficient inhibition of membrane type 1-matrix metalloproteinase.

2001 • 140 citations

High Affinity Binding of Latent Matrix Metalloproteinase-9 to the α2(IV) Chain of Collagen IV

1998 • 138 citations

Binding of tissue inhibitor of metalloproteinases 2 to two distinct sites on human 72-kDa gelatinase. Identification of a stabilization site.

1991 • 138 citations

The Helping Hand of Collagenase-3 (MMP-13): 2.7 Å Crystal Structure of its C-terminal Haemopexin-like Domain

1996 • 137 citations

A myoglobin variant with a polar substitution in a conserved hydrophobic cluster in the heme binding pocket

1997 • 130 citations

Structure-function relationship of human neutrophil collagenase: identification of regions responsible for substrate specificity and general proteinase activity.

1993 • 128 citations

Characteristics of the action of human skin fibroblast collagenase on fibrillar collagen.

1980 • 128 citations

Sequence specificity of human skin fibroblast collagenase. Evidence for the role of collagen structure in determining the collagenase cleavage site.

1987 • 120 citations

Human skin fibroblast collagenase. Assessment of activation energy and deuterium isotope effect with collagenous substrates.

1981 • 117 citations

Cancer cell-associated fibronectin induces release of matrix metalloproteinase-2 from normal fibroblasts.

2002 • 111 citations

Role of zinc-binding- and hemopexin domain-encoded sequences in the substrate specificity of collagenase and stromelysin-2 as revealed by chimeric proteins.

1993 • 109 citations

The Hemopexin-like Domain (C Domain) of Human Gelatinase A (Matrix Metalloproteinase-2) Requires Ca2+ for Fibronectin and Heparin Binding

1997 • 107 citations

A helping hand for collagenases: the haemopexin-like domain

1995 • 103 citations

Exon shuffling and other ways of module exchange

1996 • 102 citations

Human progelatinase A can be activated by autolysis at a rate that is concentration‐dependent and enhanced by heparin bound to the C‐terminal domain

1993 • 99 citations

Exons encode protein functional units

1979 • 99 citations

Domain Interactions in the Gelatinase A·TIMP-2·MT1-MMP Activation Complex

2000 • 99 citations

Mapping the collagen-binding site of human fibronectin by expression in Escherichia coli.

1986 • 95 citations

The C‐terminal (haemopexin‐like) domain structure of human gelatinase A (MMP2): structural implications for its function

1996 • 94 citations

Human fibroblast stromelysin catalytic domain: expression, purification, and characterization of a C-terminally truncated form

1991 • 92 citations

Collagen binding site in collagenase can be determined using the concept of sense-antisense peptide interactions.

1992 • 89 citations

The Involvement of the Fibronectin Type II-like Modules of Human Gelatinase A in Cell Surface Localization and Activation

1998 • 89 citations

The gelatin-binding site of human 72 kDa type IV collagenase (gelatinase A)

1994 • 88 citations

An internal cysteine plays a role in the maintenance of the latency of human fibroblast collagenase

1991 • 84 citations

Regulation of Tissue Inhibitor of Matrix Metalloproteinase Expression^a

1994 • 83 citations

Purification of recombinant human prostromelysin. Studies on heat activation to give high-Mr and low-Mr active forms, and a comparison of recombinant with natural stromelysin activities

1991 • 83 citations

Identification of the Tissue Inhibitor of Metalloproteinases-2 (TIMP-2) Binding Site on the Hemopexin Carboxyl Domain of Human Gelatinase A by Site-directed Mutagenesis

1999 • 81 citations

Matrix metalloproteinases in periodontal tissue remodelling.

1992 • 76 citations

Evidence for the involvement of type II domains in collagen binding by 72 kDa type IV procollagenase

1991 • 72 citations

Exons – original building blocks of proteins?

1991 • 72 citations

On the structure and chromosome location of the 72- and 92-kDa human type IV collagenase genes

1991 • 66 citations

Further Localization of the Gelatin-binding Determinants within Fibronectin

1989 • 65 citations

Analysis of the contribution of the hinge region of human neutrophil collagenase (HNC, MMP‐8) to stability and collagenolytic activity by alanine scanning mutagenesis

1997 • 59 citations

Collagen/collagenase interaction: Does the enzyme mimic the conformation of its own substrate?

1996 • 58 citations

The collagen‐binding site of type‐II units of bovine seminal fluid protein PDC‐109 and fibronectin

1990 • 58 citations

A Novel Matrix Metalloproteinase Gene (XMMP) Encoding Vitronectin-like Motifs Is Transiently Expressed in Xenopus laevis Early Embryo Development

1997 • 57 citations

Mutational analysis of residues in and around the active site of human fibroblast-type collagenase

1994 • 57 citations

Characterization of the peptide substrate specificities of interstitial collagenase and 92-kDa gelatinase. Implications for substrate optimization

1994 • 55 citations

Domain structure and interactions of the type I and type II modules in the gelatin-binding region of fibronectin

1991 • 53 citations

The Fibronectin-like Domain Is Required for the Type V and XI Collagenolytic Activity of Gelatinase B

1998 • 46 citations

Understanding the P₁‘ Specificity of the Matrix Metalloproteinases: Effect of S₁‘ Pocket Mutations in Matrilysin and Stromelysin-1

1996 • 45 citations

Reconstructed 19 kDa Catalytic Domain of Gelatinase A Is an Active Proteinase

1995 • 45 citations

Sea Urchin Hatching Enzyme (Envelysin): cDNA Cloning and Deprivation of Protein Substrate Specificity by Autolytic Degradation^,,

1997 • 43 citations

Characteristics of 92 kDa type IV collagenase/gelatinase produced by granulocytic leukemia cells: structure, expression of cDNA in E. coli and enzymatic properties

1994 • 41 citations

Isolation and characterization of A peptide containing the site of cleavage of the chick skin collagen α1[I] chain by animal collagenases

1979 • 39 citations

NMR of modular proteins

1998 • 37 citations

Structural Insights into the Catalytic Domains of Human Matrix Metalloprotease-2 and Human Matrix Metalloprotease-9: Implications for Substrate Specificities

1997 • 25 citations

Collagen‐binding recombinant fibronectin fragments containing type II domains

1994 • 24 citations

The Activation, Expression and Function of Gelatinase A (MMP-2).

1996 • 11 citations