Identification of Cysteine 354 of β-Tubulin as Part of the Binding Site for the A Ring of Colchicine

A gas-liquid solid phase peptide and protein sequenator.

1981 • 2,238 citations

Colchicine-binding protein of mammalian brain and its relation to microtubules

1968 • 1,140 citations

Rapid analysis of amino acid phenylthiohydantoins by high-performance liquid chromatography

1977 • 645 citations

SELECTIVE CLEAVAGE OF THE METHIONYL PEPTIDE BONDS IN RIBONUCLEASE WITH CYANOGEN BROMIDE¹

1961 • 420 citations

Structure of tubulin at 6.5 Å and location of the taxol-binding site

1995 • 369 citations

[15] Cleavage at aspartylprolyl bonds

1977 • 319 citations

Complete amino acid sequence of beta-tubulin from porcine brain.

1981 • 303 citations

Identification of a gene for β-tubulin in aspergillus nidulans

1978 • 294 citations

Interactions of colchicine with tubulin

1991 • 276 citations

3'-(p-azidobenzamido)taxol photolabels the N-terminal 31 amino acids of beta-tubulin.

1994 • 244 citations

Characterization of the Taxol Binding Site on the Microtubule

1995 • 229 citations

Separation of active tubulin and microtubule-associated proteins by ultracentrifugation and isolation of a component causing the formation of microtubule bundles

1984 • 224 citations

Tubulin sulfhydryl groups as probes and targets for antimitotic and antimicrotubule agents

1991 • 205 citations

CHO mutants resistant to colchicine, colcemid or griseofulvin have an altered β-tubulin

1980 • 201 citations

Localization of the colchicine-binding site of tubulin.

1993 • 156 citations

Direct Photoaffinity Labeling of Tubulin With Taxol

1992 • 156 citations

"Hydrophobic collapse" of taxol and Taxotere solution conformations in mixtures of water and organic solvent

1993 • 146 citations

Structure-reactivity correlations for reactions of substituted phenolate anions with acetate and formate esters

1993 • 116 citations

NMR and molecular modeling study of the conformations of taxol and of its side chain methylester in aqueous and non-aqueous solution.

1993 • 99 citations

Conformation of Taxotere® and analogues determined by NMR spectroscopy and molecular modeling studies

1993 • 78 citations

Exchangeable GTP binding site of beta-tubulin. Identification of cysteine 12 as the major site of cross-linking by direct photoaffinity labeling.

1993 • 76 citations

Structural and functional alterations in microtubule protein from Chinese hamster ovary cell mutants.

1981 • 60 citations

Direct photoaffinity labeling of tubulin with colchicine.

1991 • 59 citations

Specific affinity labelling of tubulin with bromocolchicine

1976 • 59 citations

Identification of the cysteine residue of .beta.-tubulin alkylated by the antimitotic agent 2,4-dichlorobenzyl thiocyanate, facilitated by separatio of the protein subunits of tubulin by hydrophobic column chromatography

1989 • 57 citations

Practical Protein Chemistry-A Handbook

1986 • 57 citations

The crystal structure of colchicine. A new application of magic integers to multiple-solution direct methods

1978 • 51 citations

Photoaffinity labeling of tubulin with (2-nitro-4-azidophenyl)deacetylcolchicine: direct evidence for two colchicine binding sites

1989 • 44 citations

A photoaffinity derivative of colchicine: 6'-(4'-azido-2'-nitrophenylamino)hexanoyldeacetylcolchicine. Photolabeling and location of the colchicine-binding site on the alpha-subunit of tubulin.

1985 • 44 citations

Alkylation of Estradiol 17beta-Dehydrogenase from Human Placenta with 3-Chloroacetylpyridine - Adenine Dinucleotide

1976 • 34 citations

Mechanism of action of the antimitotic drug 2,4-dichlorobenzyl thiocyanate: alkylation of sulfhydryl group(s) of ß-tubulin

1989 • 29 citations

Binding of antimitotic drugs around cysteine residues of tubulin

1978 • 23 citations

2,4-Dichlorobenzyl thiocyanate, an antimitotic agent that alters microtubule morphology.

1986 • 19 citations

Reaction of chloroacetyl‐CoA with rabbit fatty acid synthase

1982 • 19 citations

Chloroacetates of 2- and 3-demethylthiocolchicine: Specific covalent interactions with tubulin with preferential labeling of the β-subunit

1992 • 12 citations

Synthesis of ¹⁴C labelled electrophilic ligands of the colchicine binding site of tubulin: Chloroacetates of demethylthiocolchicines and of N‐acetylcolchinol; isothiocyanate of 9‐deoxy‐N‐acetylcolchinol

1993 • 5 citations