Protein engineering of disulfide bonds in subtilisin BPN'

Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

1970 • 253,849 citations

DNA sequencing with chain-terminating inhibitors

1977 • 69,181 citations

The interpretation of protein structures: Estimation of static accessibility

1971 • 5,771 citations

A new force field for molecular mechanical simulation of nucleic acids and proteins

1984 • 4,553 citations

The Anatomy and Taxonomy of Protein Structure

1981 • 3,376 citations

Calcium-dependent bacteriophage DNA infection

1970 • 2,743 citations

REQUIREMENTS FOR TRANSFORMATION IN BACILLUS SUBTILIS

1961 • 2,206 citations

A graphics model building and refinement system for macromolecules

1978 • 1,858 citations

Tertiary templates for proteins

1987 • 1,485 citations

Oligonucleotide-directed mutagenesis using M13-derived vectors: an efficient and general procedure for the production of point mutations in any fragment of DNA

1982 • 764 citations

A sensitive new substrate for chymotrypsin

1979 • 584 citations

Structure of Subtilisin BPN′ at 2.5 Å Resolution

1969 • 543 citations

Different base/base mismatches are corrected with different efficiencies by the methyl-directed DNA mismatch-repair system of E. coli

1984 • 468 citations

Cloning, sequencing, and secretion of Bacillusamyloliquefacienssubtillisin inBacillus subtilis

1983 • 357 citations

Engineering an enzyme by site-directed mutagenesis to be resistant to chemical oxidation.

1985 • 334 citations

Disulfide Bond Engineered into T4 Lysozyme: Stabilization of the Protein Toward Thermal Inactivation

1984 • 311 citations

Correlation between sites of limited proteolysis and segmental mobility in thermolysin

1986 • 297 citations

Mutant forms of staphylococcal nuclease with altered patterns of guanidine hydrochloride and urea denaturation

1986 • 275 citations

Cloning of the neutral protease gene of Bacillus subtilis and the use of the cloned gene to create an in vitro-derived deletion mutation

1984 • 257 citations

In vivo formation and stability of engineered disulfide bonds in subtilisin.

1986 • 232 citations

Protein engineering of subtilisin BPN': enhanced stabilization through the introduction of two cysteines to form a disulfide bond

1987 • 224 citations

Engineering Enzyme Specificity by "Substrate-Assisted Catalysis"

1987 • 219 citations

Directed Mutagenesis of Dihydrofolate Reductase

1983 • 210 citations

The crystallographically determined structures of atypical strained disulfides engineered into subtilisin.

1986 • 182 citations

The three-dimensional structure of Bacillus amyloliquefaciens subtilisin at 1.8 A and an analysis of the structural consequences of peroxide inactivation.

1988 • 178 citations

Role of primary structure and disulfide bond formation in beta-lactamase secretion

1983 • 170 citations

Disulfide bonds and thermal stability in T4 lysozyme.

1988 • 165 citations

An engineered intersubunit disulfide enhances the stability and DNA binding of the N-terminal domain of .lambda. repressor

1986 • 149 citations

Proteases of enhanced stability: Characteization of a thermostable variant of subtilisin

1986 • 147 citations

Role of bound calcium ions in thermostable, proteolytic enzymes. Separation of intrinsic and calcium ion contributions to the kinetic thermal stability

1976 • 146 citations

Bacteriophage lambda cro mutations: effects on activity and intracellular degradation.

1986 • 145 citations

Importance of hydrogen-bond formation in stabilizing the transition state of subtilisin

1986 • 136 citations

An engineered disulfide bond in dihydrofolate reductase

1987 • 133 citations

Unfolding and refolding of the constant fragment of the immunoglobulin light chain

1982 • 124 citations

Influence of an extrinsic crosslink on the folding pathway of ribonuclease A. Conformational and thermodynamic analysis of crosslinked (7-lysine, 41-lysine)-ribonuclease A

1984 • 119 citations

Harnessing disulfide bonds using protein engineering

1987 • 116 citations

Subtilisin BPN'

1965 • 108 citations

Computer-aided model-building strategies for protein design

1986 • 107 citations

Subtilisin Novo. The Three-Dimensional Structure and Its Comparison with Subtilisin BPN'

1972 • 104 citations

Energetics of folding and unfolding of pancreatic trypsin inhibitor

1977 • 89 citations

Unfolding and refolding of the reduced constant fragment of the immunoglobulin light chain

1982 • 86 citations

Thermodynamics of protein cross-links

1978 • 85 citations

Three-dimensional structure of fungal proteinase K reveals similarity to bacterial subtilisin.

1984 • 85 citations

Kinetic analysis of the folding and unfolding of a mutant form of bovine pancreatic trypsin inhibitor lacking the cysteine-14 and -38 thiols

1988 • 84 citations

Improvement in the alkaline stability of subtilisin using an efficient random mutagenesis and screening procedure

1987 • 82 citations

The disulphide folding pathway of ribonuclease T1

1986 • 65 citations

Mutants of Bovine Pancreatic Trypsin Inhibitor Lacking Cysteines 14 and 38 Can Fold Properly

1987 • 65 citations

The Protein Folding Problem

1988 • 44 citations

Noncovalent Forces Determining Protein Structure

1979 • 16 citations