Importance of hydrogen-bond formation in stabilizing the transition state of subtilisin

Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

1970 • 253,849 citations

Experiments in molecular genetics

1972 • 28,260 citations

Methods in Enzymology , Vol

1966 • 26,261 citations

[2] New M13 vectors for cloning

1983 • 5,402 citations

A new pair of M13 vectors for selecting either DNA strand of double-digest restriction fragments

1982 • 3,041 citations

Supercoil Sequencing: A Fast and Simple Method for Sequencing Plasmid DNA

1985 • 2,505 citations

Hydrogen bonding and biological specificity analysed by protein engineering

1985 • 1,050 citations

Biological Macromolecules and Assemblies

1987 • 400 citations

Subtilisin. Stereochemical mechanism involving transition-state stabilization

1972 • 376 citations

Cloning, sequencing, and secretion of Bacillusamyloliquefacienssubtillisin inBacillus subtilis

1983 • 357 citations

Cassette mutagenesis: an efficient method for generation of multiple mutations at defined sites

1985 • 270 citations

Direct determination of the protonation states of aspartic acid-102 and histidine-57 in the tetrahedral intermediate of the serine proteases: neutron structure of trypsin

1981 • 266 citations

The mechanism of the reaction of chymotrypsin with p-nitrophenyl acetate

1956 • 259 citations

A Family of Protein-Cutting Proteins

1974 • 157 citations

16 Subtilisins: Primary Structure, Chemical and Physical Properties

1971 • 155 citations

Bacillus subtilis Requires a "Stringent" Shine-Dalgarno Region for Gene Expression

1984 • 155 citations

A large increase in enzyme–substrate affinity by protein engineering

1984 • 147 citations

Kinetics of subtilisin and thiolsubtilisin

1983 • 139 citations

The Reactivity of Thiol-subtilisin, an Enzyme Containing a Synthetic Functional Group^*

1967 • 120 citations

Structure-Reactivity Correlations and General Acid-Base Catalysis in Enzymic Transacylation Reactions

1972 • 35 citations