Protein engineering of subtilisin BPN': enhanced stabilization through the introduction of two cysteines to form a disulfide bond

Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

1970 • 253,849 citations

The Anatomy and Taxonomy of Protein Structure

1981 • 3,376 citations

Protein denaturation. C. Theoretical models for the mechanism of denaturation.

1970 • 1,550 citations

A thermodynamic approach to the problem of stabilization of globular protein structure: A calorimetric study

1974 • 1,384 citations

[32] Oligonucleotide-directed mutagenesis of DNA fragments cloned into M13 vectors

1983 • 1,093 citations

Disulphide bridges in globular proteins

1981 • 770 citations

A sensitive new substrate for chymotrypsin

1979 • 584 citations

Theory of Elastic Mechanisms in Fibrous Proteins

1956 • 497 citations

Cloning, sequencing, and secretion of Bacillusamyloliquefacienssubtillisin inBacillus subtilis

1983 • 357 citations

Disulfide Bond Engineered into T4 Lysozyme: Stabilization of the Protein Toward Thermal Inactivation

1984 • 311 citations

Genes for alkaline protease and neutral protease from Bacillus amyloliquefaciens contain a large open reading frame between the regions coding for signal sequence and mature protein

1984 • 282 citations

Site-directed mutagenesis and the role of the oxyanion hole in subtilisin.

1986 • 236 citations

In vivo formation and stability of engineered disulfide bonds in subtilisin.

1986 • 232 citations

Directed Mutagenesis of Dihydrofolate Reductase

1983 • 210 citations

Thermal denaturation of Streptomyces subtilisin inhibitor, subtilisin BPN', and the inhibitor-subtilisin complex

1981 • 192 citations

Role of primary structure and disulfide bond formation in beta-lactamase secretion

1983 • 170 citations

Effect of single amino acid replacements on the thermal stability of the NH2-terminal domain of phage lambda repressor.

1984 • 153 citations

An engineered intersubunit disulfide enhances the stability and DNA binding of the N-terminal domain of .lambda. repressor

1986 • 149 citations

Role of bound calcium ions in thermostable, proteolytic enzymes. Separation of intrinsic and calcium ion contributions to the kinetic thermal stability

1976 • 146 citations

Statistical mechanics of noncovalent bonds in polyamino acids. VIII. Covalent loops in proteins

1965 • 130 citations

Influence of an extrinsic crosslink on the folding pathway of ribonuclease A. Conformational and thermodynamic analysis of crosslinked (7-lysine, 41-lysine)-ribonuclease A

1984 • 119 citations

Complete primary structure of thermitase from Thermoactinomyces vulgaris and its structural features related to the subtilisin‐type proteinases

1985 • 114 citations

Computer-aided model-building strategies for protein design

1986 • 107 citations

Site-saturation studies of beta-lactamase: production and characterization of mutant beta-lactamases with all possible amino acid substitutions at residue 71.

1986 • 73 citations

Effect of single amino acid substitutions on the thermal stability of the .alpha. subunit of tryptophan synthase

1980 • 60 citations

Protease-deficient Bacillus subtilis host strains for production of Staphylococcal protein A

1987 • 37 citations

An intramolecular crosslinkage of lysozyme. Formation of crosslinks between lysine-1 and histidine-15 with bis(bromoacetamide) derivatives by a two-stage reaction procedure and properties of the resulting derivatives

1985 • 37 citations

Inhibition of subtilisin by substituted arylboronic acids

1981 • 24 citations

The 2-A resolution structure of a thermostable ribonuclease A chemically cross-linked between lysine residues 7 and 41.

1985 • 21 citations