Engineering an enzyme by site-directed mutagenesis to be resistant to chemical oxidation.

An Algorithm for Least-Squares Estimation of Nonlinear Parameters

1963 • 29,357 citations

Atlas of protein sequence and structure

1965 • 4,462 citations

REQUIREMENTS FOR TRANSFORMATION IN BACILLUS SUBTILIS

1961 • 2,206 citations

Laboratory techniques in biochemistry and molecular biology

1973 • 2,129 citations

Chemical Modification of Proteins

2012 • 1,097 citations

Oligonucleotide-directed mutagenesis using M13-derived vectors: an efficient and general procedure for the production of point mutations in any fragment of DNA

1982 • 764 citations

A sensitive new substrate for chymotrypsin

1979 • 584 citations

Structure of Subtilisin BPN′ at 2.5 Å Resolution

1969 • 543 citations

Chemical Modification of Proteins

1974 • 419 citations

Cloning, sequencing, and secretion of Bacillusamyloliquefacienssubtillisin inBacillus subtilis

1983 • 357 citations

Enzymatic reduction of protein-bound methionine sulfoxide.

1981 • 345 citations

Biochemistry and physiological role of methionine sulfoxide residues in proteins

1983 • 295 citations

Cloning of the neutral protease gene of Bacillus subtilis and the use of the cloned gene to create an in vitro-derived deletion mutation

1984 • 257 citations

Synthesis in yeast of a functional oxidation-resistant mutant of human α1-antitrypsin

1984 • 209 citations

Oligonucleotide directed mutagenesis of the human β-globin gene: a general method for producing specific point mutations in cloned DNA

1981 • 207 citations

X-ray crystallographic study of the binding of peptide chloromethyl ketone inhibitors to subtilisin BPN

1972 • 168 citations

Subtilisin BPN'

1965 • 108 citations

The Effect on Subtilisin Activity of Oxidizing a Methionine Residue

1969 • 107 citations

Subtilisin Novo. The Three-Dimensional Structure and Its Comparison with Subtilisin BPN'

1972 • 104 citations