Proteases of enhanced stability: Characteization of a thermostable variant of subtilisin

A thermodynamic approach to the problem of stabilization of globular protein structure: A calorimetric study

1974 • 1,384 citations

[32] Oligonucleotide-directed mutagenesis of DNA fragments cloned into M13 vectors

1983 • 1,093 citations

Electrostatic Effects in Proteins

1978 • 734 citations

Proteinase K from Tritirachium album Limber

1974 • 607 citations

A sensitive new substrate for chymotrypsin

1979 • 584 citations

Thermal stability and protein structure

1979 • 518 citations

Local mutagenesis: a method for generating viral mutants with base substitutions in preselected regions of the viral genome.

1978 • 282 citations

Genes for alkaline protease and neutral protease from Bacillus amyloliquefaciens contain a large open reading frame between the regions coding for signal sequence and mature protein

1984 • 282 citations

Isolation of a thermostable enzyme variant by cloning and selection in a thermophile.

1986 • 279 citations

The beta bulge: a common small unit of nonrepetitive protein structure.

1978 • 237 citations

Site-directed mutagenesis and the role of the oxyanion hole in subtilisin.

1986 • 236 citations

In vivo formation and stability of engineered disulfide bonds in subtilisin.

1986 • 232 citations

Protein engineering of subtilisin BPN': enhanced stabilization through the introduction of two cysteines to form a disulfide bond

1987 • 224 citations

Molecular basis of thermostability in the lysozyme from bacteriophage T4

1979 • 212 citations

Directed Mutagenesis of Dihydrofolate Reductase

1983 • 210 citations

Cloning, sequencing and expression of subtilisin Carlsberg fromBacillus licheniformis

1985 • 204 citations

Thermal denaturation of Streptomyces subtilisin inhibitor, subtilisin BPN', and the inhibitor-subtilisin complex

1981 • 192 citations

GENETIC ANALYSIS OF STAPHYLOCOCCAL NUCLEASE: IDENTIFICATION OF THREE INTRAGENIC "GLOBAL" SUPPRESSORS OF NUCLEASE-MINUS MUTATIONS

1985 • 189 citations

Computing in crystallography

1981 • 159 citations

Effect of single amino acid replacements on the thermal stability of the NH2-terminal domain of phage lambda repressor.

1984 • 153 citations

Thermodynamic stability and point mutations of bacteriophage T4 lysozyme

1984 • 149 citations

Role of bound calcium ions in thermostable, proteolytic enzymes. Separation of intrinsic and calcium ion contributions to the kinetic thermal stability

1976 • 146 citations

Heat Stability of a Tetrameric Enzyme, D-Glyceraldehyde-3-PhosphateDehydrogenase

1980 • 119 citations

Complete primary structure of thermitase from Thermoactinomyces vulgaris and its structural features related to the subtilisin‐type proteinases

1985 • 114 citations

Crystal and molecular structure of the inhibitor eglin from leeches in complex with subtilisin Carlsberg

1985 • 96 citations

Unpaired cysteine-54 interferes with the ability of an engineered disulfide to stabilize T4 lysozyme

1986 • 91 citations

Three-dimensional structure of fungal proteinase K reveals similarity to bacterial subtilisin.

1984 • 85 citations

Crystallization and Crystal Data of Monellin

1975 • 77 citations

Site-saturation studies of beta-lactamase: production and characterization of mutant beta-lactamases with all possible amino acid substitutions at residue 71.

1986 • 73 citations

Effect of single amino acid substitutions on the thermal stability of the .alpha. subunit of tryptophan synthase

1980 • 60 citations

Subtilisin Amylosacchariticus

1972 • 55 citations

Active-site mutants of .beta.-lactamase: use of an inactive double mutant to study requirements for catalysis

1986 • 52 citations

Influence of calcium binding on the thermal stability of ‘thermitase’, a serine protease from Thermoactinomyces vulgaris

1981 • 42 citations

Determination of the Complete Amino-Acid Sequence of Subtilisin DY and its Comparison with the Primary Structures of the Subtilisins BPN’, Carlsberg and Amylosacchariticus

1985 • 37 citations

Protease-deficient Bacillus subtilis host strains for production of Staphylococcal protein A

1987 • 37 citations

Effect of single amino acid substitutions at the same position on stability of a two-domain protein

1982 • 37 citations

Proteinase K fromTritirachium albumLimber. I. Molecular Mass and Sequence around the Active Site Serine Residue

1985 • 35 citations

A comparison of crystallographic data of the subtilopeptidases B and C

1967 • 25 citations

Inhibition of subtilisin by substituted arylboronic acids

1981 • 24 citations

Complete amino acid sequence of alkaline mesentericopeptidase

1986 • 22 citations

[34] Thermomycolin

1976 • 14 citations