In vivo formation and stability of engineered disulfide bonds in subtilisin.

Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

1970 • 253,849 citations

Experiments in molecular genetics

1972 • 28,260 citations

Tissue sulfhydryl groups

1959 • 25,130 citations

A rapid alkaline extraction procedure for screening recombinant plasmid DNA

1979 • 15,511 citations

Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencing

1980 • 3,476 citations

The Anatomy and Taxonomy of Protein Structure

1981 • 3,376 citations

A new pair of M13 vectors for selecting either DNA strand of double-digest restriction fragments

1982 • 3,041 citations

Calcium-dependent bacteriophage DNA infection

1970 • 2,743 citations

REQUIREMENTS FOR TRANSFORMATION IN BACILLUS SUBTILIS

1961 • 2,206 citations

Dithiothreitol, a New Protective Reagent for SH Groups^*

1964 • 1,933 citations

Experimental and Theoretical Aspects of Protein Folding

1975 • 965 citations

Base composition-independent hybridization in tetramethylammonium chloride: a method for oligonucleotide screening of highly complex gene libraries.

1985 • 818 citations

Disulphide bridges in globular proteins

1981 • 770 citations

Oligonucleotide-directed mutagenesis using M13-derived vectors: an efficient and general procedure for the production of point mutations in any fragment of DNA

1982 • 764 citations

A sensitive new substrate for chymotrypsin

1979 • 584 citations

Structure of Subtilisin BPN′ at 2.5 Å Resolution

1969 • 543 citations

Stabilization of Enzymes against Thermal Inactivation

1983 • 458 citations

Rate constants and equilibrium constants for thiol-disulfide interchange reactions involving oxidized glutathione

1980 • 400 citations

Cloning, sequencing, and secretion of Bacillusamyloliquefacienssubtillisin inBacillus subtilis

1983 • 357 citations

Engineering an enzyme by site-directed mutagenesis to be resistant to chemical oxidation.

1985 • 334 citations

Disulfide Bond Engineered into T4 Lysozyme: Stabilization of the Protein Toward Thermal Inactivation

1984 • 311 citations

Kinetic role of a meta-stable native-like two-disulphide species in the folding transition of bovine pancreatic trypsin inhibitor

1984 • 308 citations

Cassette mutagenesis: an efficient method for generation of multiple mutations at defined sites

1985 • 270 citations

Cloning of the neutral protease gene of Bacillus subtilis and the use of the cloned gene to create an in vitro-derived deletion mutation

1984 • 257 citations

Directed Mutagenesis of Dihydrofolate Reductase

1983 • 210 citations

Oligonucleotide directed mutagenesis of the human β-globin gene: a general method for producing specific point mutations in cloned DNA

1981 • 207 citations

Role of primary structure and disulfide bond formation in beta-lactamase secretion

1983 • 170 citations

Bacillus subtilis Requires a "Stringent" Shine-Dalgarno Region for Gene Expression

1984 • 155 citations

Efficient site-directed mutagenesis by simultaneous use of two primers

1983 • 154 citations

Effect of single amino acid replacements on the thermal stability of the NH2-terminal domain of phage lambda repressor.

1984 • 153 citations

Conformational changes associated with proteolytic processing of presecretory proteins allow glutathione-catalyzed formation of native disulfide bonds.

1982 • 125 citations

Subtilisin BPN'

1965 • 108 citations

Subtilisin Novo. The Three-Dimensional Structure and Its Comparison with Subtilisin BPN'

1972 • 104 citations

Is protein turnover thermodynamically controlled?

1978 • 97 citations

Energetics of folding and unfolding of pancreatic trypsin inhibitor

1977 • 89 citations

Roles of the 29-138 disulfide bond of subtype A of human .alpha. interferon in its antiviral activity and conformational stability

1984 • 66 citations

In VitroDeletional Mutagenesis for Bacterial Production of the 20,000-Dalton Form of Human Pituitary Growth Hormone.

1983 • 53 citations

Chromatography and activity of thiol-subtilisin

1969 • 31 citations

Prolipoprotein modification and processing in Escherichia coli

1984 • 22 citations

Circular dichroism and gel filtration behavior of subtilisin enzymes in concentrated solutions of guanidine hydrochloride

1975 • 19 citations

Effects of pH and urea on the conformational properties of subtilisin DY

1982 • 14 citations