Manipulation of a Nuclear NAD+ Salvage Pathway Delays Aging without Altering Steady-state NAD+ Levels

A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae.

1989 • 8,705 citations

Additional modules for versatile and economical PCR-based gene deletion and modification in Saccharomyces cerevisiae

1998 • 5,253 citations

A comprehensive analysis of protein–protein interactions in Saccharomyces cerevisiae

2000 • 4,734 citations

Functional Characterization of the S. cerevisiae Genome by Gene Deletion and Parallel Analysis

1999 • 3,929 citations

Transcriptional silencing and longevity protein Sir2 is an NAD-dependent histone deacetylase

2000 • 3,355 citations

The SIR2/3/4 complex and SIR2 alone promote longevity in Saccharomyces cerevisiae by two different mechanisms

1999 • 2,199 citations

Increased dosage of a sir-2 gene extends lifespan in Caenorhabditis elegans

2001 • 1,892 citations

Requirement of NAD and SIR2 for Life-Span Extension by Calorie Restriction in Saccharomyces cerevisiae

2000 • 1,756 citations

A new efficient gene disruption cassette for repeated use in budding yeast

1996 • 1,609 citations

Extrachromosomal rDNA Circles— A Cause of Aging in Yeast

1997 • 1,477 citations

Extension of Life-Span by Loss of CHICO, a Drosophila Insulin Receptor Substrate Protein

2001 • 1,398 citations

Position effect at S. cerevisiae telomeres: Reversible repression of Pol II transcription

1990 • 1,343 citations

Genetic pathways that regulate ageing in model organisms

2000 • 1,341 citations

Understanding the control of metabolism

1997 • 1,265 citations

Evolution of senescence: late survival sacrificed for reproduction

1991 • 1,066 citations

The silencing protein SIR2 and its homologs are NAD-dependent protein deacetylases

2000 • 946 citations

Life Span of Individual Yeast Cells

1959 • 754 citations

Sir2 links chromatin silencing, metabolism, and aging

2000 • 725 citations

Exit from Mitosis Is Triggered by Tem1-Dependent Release of the Protein Phosphatase Cdc14 from Nucleolar RENT Complex

1999 • 720 citations

A phylogenetically conserved NAD ⁺ -dependent protein deacetylase activity in the Sir2 protein family

2000 • 703 citations

Caloric restriction and aging: an update

2000 • 616 citations

An unusual form of transcriptional silencing in yeast ribosomal DNA.

1997 • 590 citations

Silent information regulator 2 family of NAD- dependent histone/protein deacetylases generates a unique product, 1- O- acetyl-ADP-ribose

2000 • 541 citations

A Conserved Regulatory System for Aging

2001 • 526 citations

Genomewide studies of histone deacetylase function in yeast

2000 • 415 citations

Accelerated Aging and Nucleolar Fragmentation in Yeast sgs1 Mutants

1997 • 399 citations

Elimination of Replication Block Protein Fob1 Extends the Life Span of Yeast Mother Cells

1999 • 398 citations

Net1, a Sir2-Associated Nucleolar Protein Required for rDNA Silencing and Nucleolar Integrity

1999 • 383 citations

An intervention resembling caloric restriction prolongs life span and retards aging in yeast

2000 • 377 citations

Identification of a small molecule inhibitor of Sir2p

2001 • 337 citations

MEC1-Dependent Redistribution of the Sir3 Silencing Protein from Telomeres to DNA Double-Strand Breaks

1999 • 332 citations

Daughter cells of Saccharomyces cerevisiae from old mothers display a reduced life span.

1994 • 309 citations

Silencers, silencing, and heritable transcriptional states

1992 • 296 citations

Role of NAD+ in the Deacetylase Activity of the SIR2-like Proteins

2000 • 251 citations

Coupling of histone deacetylation to NAD breakdown by the yeast silencing protein Sir2: Evidence for acetyl transfer from substrate to an NAD breakdown product

2000 • 245 citations

CYTOCHEMICAL STUDIES

1952 • 227 citations

A Genetic Screen for Ribosomal DNA Silencing Defects Identifies Multiple DNA Replication and Chromatin-Modulating Factors

1999 • 227 citations

Caloric restriction of rhesus monkeys lowers oxidative damage in skeletal muscle

2000 • 215 citations

A cDNA encoding a human CCAAT-binding protein cloned by functional complementation in yeast.

1991 • 198 citations

Enhanced Gluconeogenesis and Increased Energy Storage as Hallmarks of Aging in Saccharomyces cerevisiae

2001 • 181 citations

Mechanisms of life span determination in Caenorhabditis elegans☆

1999 • 177 citations

Sip2p and its partner Snf1p kinase affect aging in S. cerevisiae

2000 • 165 citations

A cytosolic NAD-dependent deacetylase, Hst2p, can modulate nucleolar and telomeric silencing in yeast

2001 • 163 citations

Effects of Mutations in DNA Repair Genes on Formation of Ribosomal DNA Circles and Life Span in Saccharomyces cerevisiae

1999 • 153 citations

Some Aspects of Cell Division in Saccharomyces cerevisiae

1950 • 137 citations

Characterization of a human gene with sequence homology to Saccharomyces cerevisiae SIR2

1999 • 130 citations

Net1 Stimulates RNA Polymerase I Transcription and Regulates Nucleolar Structure Independently of Controlling Mitotic Exit

2001 • 130 citations

Identification and functional analysis of the Saccharomyces cerevisiae nicotinamidase gene, PNC1

2001 • 124 citations

Mutations in signal transduction proteins increase stress resistance and longevity in yeast, nematodes, fruit flies, and mammalian neuronal cells

1999 • 123 citations

Interactions between three common subunits of yeast RNA polymerases I and III.

1993 • 114 citations

A novel multi-purpose cassette for repeated integrative epitope tagging of genes in Saccharomyces cerevisiae

2000 • 98 citations

The Sir2 protein family: A novel deacetylase for gene silencing and more

2000 • 83 citations

A gene with specific and global effects on recombination of sequences from tandemly repeated genes in Saccharomyces cerevisiae.

1993 • 80 citations

Effect of Replicative Age on Transcriptional Silencing Near Telomeres inSaccharomyces cerevisiae

1996 • 75 citations

Structure of nicotinamide mononucleotide adenylyltransferase: a key enzyme in NAD+ biosynthesis

2000 • 69 citations

Identification and characterization of YLR328W, the Saccharomyces cerevisiae structural gene encoding NMN adenylyltransferase. Expression and characterization of the recombinant enzyme

1999 • 57 citations

Pyridine nucleotide cycle of Salmonella typhimurium: isolation and characterization of pncA, pncB, and pncC mutants and utilization of exogenous nicotinamide adenine dinucleotide

1979 • 56 citations

Identification, Characterization, and Crystal Structure ofBacillus subtilis Nicotinic Acid Mononucleotide Adenylyltransferase

2002 • 54 citations

Insights into Ligand Binding and Catalysis of a Central Step in NAD+ Synthesis

2001 • 53 citations

Variation of cofactor levels in Escherichia coli. Sequence analysis and expression of the pncB gene encoding nicotinic acid phosphoribosyltransferase.

1990 • 48 citations

New clues to old yeast

2001 • 46 citations

Conversion of a Cosubstrate to an Inhibitor: Phosphorylation Mutants of Nicotinic Acid Phosphoribosyltransferase

1998 • 31 citations

Structural gene for NAD synthetase in Salmonella typhimurium

1988 • 30 citations

Genetic analysis of aging in Saccharomyces cerevisiae

1996 • 28 citations

A cross-linked control system I. Properties of a triphosphate-dependent nicotinic acid mononucleotide pyrophosphorylase from Bacillus subtilis

1964 • 23 citations

[2] Energy coupling through molecular discrimination: Nicotinate phosphoribosyltransferase

1999 • 22 citations

Cloning and nucleic acid sequence of the Salmonella typhimurium pncB gene and structure of nicotinate phosphoribosyltransferase

1991 • 21 citations