Simian Immunodeficiency Virus and Human Immunodeficiency Virus Type 1 Nef Proteins Show Distinct Patterns and Mechanisms of Src Kinase Activation

Production of high-titer helper-free retroviruses by transient transfection.

1993 • 2,280 citations

Importance of the nef gene for maintenance of high virus loads and for development of AIDS

1991 • 1,613 citations

Crystal structure of the Src family tyrosine kinase Hck

1997 • 1,165 citations

Endocytosis of major histocompatibility complex class I molecules is induced by the HIV–1 Nef protein

1996 • 1,041 citations

Serine phosphorylation-independent downregulation of cell-surface CD4 by nef

1991 • 760 citations

Activation of the Sire-family tyrosine kinase Hck by SH3 domain displacement

1997 • 601 citations

Proline-rich (PxxP) motifs in HIV-1 Nef bind to SH3 domains of a subset of Src kinases and are required for the enhanced growth of Nef+ viruses but not for down-regulation of CD4.

1995 • 557 citations

HIV F/3' orf encodes a phosphorylated GTP-binding protein resembling an oncogene product

1987 • 523 citations

Crystal Structure of the Conserved Core of HIV-1 Nef Complexed with a Src Family SH3 Domain

1996 • 433 citations

Nef stimulates human immunodeficiency virus type 1 proviral DNA synthesis

1995 • 385 citations

Nef Interacts with the μ Subunit of Clathrin Adaptor Complexes and Reveals a Cryptic Sorting Signal in MHC I Molecules

1998 • 383 citations

The solution structure of HIV-1 Nef reveals an unexpected fold and permits delineation of the binding surface for the SH3 domain of Hck tyrosine protein kinase

1996 • 336 citations

Downregulation of cell-surface CD4 expression by simian immunodeficiency virus Nef prevents viral super infection.

1993 • 291 citations

Altered T cell activation and development in transgenic mice expressing the HIV-1 nef gene.

1993 • 282 citations

Human immunodeficiency virus type 1 Nef increases the efficiency of reverse transcription in the infected cell

1995 • 266 citations

Identification of a nef allele that causes lymphocyte activation and acute disease in Macaque monkeys

1995 • 252 citations

A dileucine motif in HIV-1 Nef is essential for sorting into clathrin-coated pits and for downregulation of CD4

1998 • 232 citations

The crystal structure of HIV-1 Nef protein bound to the Fyn kinase SH3 domain suggests a role for this complex in altered T cell receptor signaling

1997 • 208 citations

Separable functions of Nef disrupt two aspects of T cell receptor machinery: CD4 expression and CD3 signaling

1997 • 206 citations

SH3-mediated Hck Tyrosine Kinase Activation and Fibroblast Transformation by the Nef Protein of HIV-1

1997 • 201 citations

Requirement of human immunodeficiency virus type 1 nef for in vivo replication and pathogenicity

1994 • 195 citations

Expression of the type 1 human immunodeficiency virus Nef protein in T cells prevents antigen receptor-mediated induction of interleukin 2 mRNA.

1991 • 192 citations

Dissociation of the CD4 downregulation and viral infectivity enhancement functions of human immunodeficiency virus type 1 Nef

1995 • 191 citations

Physical and Functional Interaction of Nef with Lck

1996 • 165 citations

The N-Terminus of Nef from HIV-1/SIV Associates with a Protein Complex Containing Lck and a Serine Kinase

1997 • 156 citations

Expression of the human immunodeficiency virus type 1 (HIV-1) nef gene during HIV-1 production increases progeny particle infectivity independently of gp160 or viral entry

1995 • 140 citations

Cytoplasmic localization of the HTLV-III 3′orf protein in cultured T cells

1986 • 134 citations

Human immunodeficiency virus type 1 Nef binds directly to Lck and mitogen-activated protein kinase, inhibiting kinase activity

1996 • 134 citations

Human immunodeficiency virus type 1 Nef protein inhibits activation pathways in peripheral blood mononuclear cells and T-cell lines

1995 • 128 citations

HIV-1 Nef Association with Cellular Serine Kinase Correlates with Enhanced Virion Infectivity and Efficient Proviral DNA Synthesis

1996 • 94 citations

Association of simian immunodeficiency virus Nef with cellular serine/threonine kinases is dispensable for the development of AIDS in rhesus macaques

1997 • 92 citations

CD4 cell surface downregulation in HIV-1 Nef transgenic mice is a consequence of intracellular sequestration.

1993 • 87 citations

Severe immunodeficiency associated with a human immunodeficiency virus 1 NEF/3'-long terminal repeat transgene.

1994 • 84 citations

Specific Th1 cytokine down-regulation associated with primary clinically derived human immunodeficiency virus type 1 Nef gene-induced expression.

1996 • 76 citations

Nef 27, but Not the Nef 25 Isoform of Human Immunodeficiency Virus-Type 1 pNL4.3 Down-Regulates Surface CD4 and IL-2R Expression in Peripheral Blood Mononuclear Cells and Transformed T Cells

1994 • 62 citations

Activation of the T-cell receptor signaling pathway by Nef from an aggressive strain of simian immunodeficiency virus

1997 • 58 citations

Biochemical and biological comparison of HIV-1 NEF and ras gene products

1991 • 52 citations

The Human Immunodeficiency Virus Type 1 NEF Protein Binds the Src-Related Tyrosine Kinase Lck SH2 Domain Through a Novel Phosphotyrosine Independent Mechanism

1998 • 45 citations

The nef gene products of both simian and human immunodeficiency viruses enhance virus infectivity and are functionally interchangeable

1997 • 42 citations

Large-scale production and characterization of recombinant human immunodeficiency virus type 1 Nef

1994 • 24 citations

Evidence for intact CD28 signaling in T cell hyporesponsiveness induced by the HIV‐1 nef gene

1996 • 21 citations

DNA Tumor Viruses and Src Family Tyrosine Kinases, an Intimate Relationship

1997 • 17 citations