Separable functions of Nef disrupt two aspects of T cell receptor machinery: CD4 expression and CD3 signaling

Molecular Cloning: A Laboratory Manual

2001 • 133,517 citations

Molecular cloning: A laboratory manual

1990 • 85,659 citations

SV40-transformed simian cells support the replication of early SV40 mutants

1981 • 2,564 citations

Signal transduction by lymphocyte antigen receptors

1994 • 2,145 citations

Importance of the nef gene for maintenance of high virus loads and for development of AIDS

1991 • 1,613 citations

Genomic Structure of an Attenuated Quasi Species of HIV-1 from a Blood Transfusion Donor and Recipients

1995 • 1,158 citations

Absence of Intact nef Sequences in a Long-Term Survivor with Nonprogressive HIV-1 Infection

1995 • 1,008 citations

Serine phosphorylation-independent downregulation of cell-surface CD4 by nef

1991 • 760 citations

Nef induces CD4 endocytosis: Requirement for a critical dileucine motif in the membrane-proximal CD4 cytoplasmic domain

1994 • 701 citations

Differential transcriptional activation by Oct-1 and Oct-2: Interdependent activation domains induce Oct-2 phosphorylation

1990 • 696 citations

Proline-rich (PxxP) motifs in HIV-1 Nef bind to SH3 domains of a subset of Src kinases and are required for the enhanced growth of Nef+ viruses but not for down-regulation of CD4.

1995 • 557 citations

The T Cell Receptor as a Multicomponent Signalling Machine: CD4/CD8 Coreceptors and CD45 in T Cell Activation

1992 • 539 citations

The human immunodeficiency virus-1 nef gene product: a positive factor for viral infection and replication in primary lymphocytes and macrophages.

1994 • 536 citations

Inhibition of cell proliferation by p107, a relative of the retinoblastoma protein.

1993 • 531 citations

Impaired proliferation of peripheral B cells and indication of autoimmune disease in lyn-deficient mice

1995 • 475 citations

Crystal Structure of the Conserved Core of HIV-1 Nef Complexed with a Src Family SH3 Domain

1996 • 433 citations

The importance of nef in the induction of human immunodeficiency virus type 1 replication from primary quiescent CD4 lymphocytes.

1994 • 401 citations

Nef stimulates human immunodeficiency virus type 1 proviral DNA synthesis

1995 • 385 citations

The solution structure of HIV-1 Nef reveals an unexpected fold and permits delineation of the binding surface for the SH3 domain of Hck tyrosine protein kinase

1996 • 336 citations

The Complete Nucleotide Sequence of a Pathogenic Molecular Clone of Simian Immunodeficiency Virus

1990 • 335 citations

HIV-1 nef leads to inhibition or activation of T cells depending on its intracellular localization

1994 • 305 citations

Resting and anergic B cells are defective in CD28-dependent costimulation of naive CD4+ T cells.

1994 • 299 citations

Downregulation of cell-surface CD4 expression by simian immunodeficiency virus Nef prevents viral super infection.

1993 • 291 citations

Altered T cell activation and development in transgenic mice expressing the HIV-1 nef gene.

1993 • 282 citations

Identification of a nef allele that causes lymphocyte activation and acute disease in Macaque monkeys

1995 • 252 citations

Analysis of human immunodeficiency virus type 1 nef gene sequences present in vivo

1993 • 241 citations

Functional overlap in the src gene family: inactivation of hck and fgr impairs natural immunity.

1994 • 232 citations

Human immunodeficiency virus type 1 Nef associateswith a cellular serine kinase in T lymphocytes.

1994 • 227 citations

High frequency of defective nef alleles in a long-term survivor with nonprogressive human immunodeficiency virus type 1 infection

1996 • 222 citations

Expression of the type 1 human immunodeficiency virus Nef protein in T cells prevents antigen receptor-mediated induction of interleukin 2 mRNA.

1991 • 192 citations

CD4 down-regulation by nef alleles isolated from human immunodeficiency virus type 1-infected individuals.

1993 • 186 citations

Human immunodeficiency virus type 1 Nef-induced down-modulation of CD4 is due to rapid internalization and degradation of surface CD4

1994 • 179 citations

Characterization of a cell surface-expressed disulfide-linked dimer involved in murine T cell activation.

1988 • 160 citations

Natural HIV-1 NEF accelerates virus replication in primary human lymphocytes

1992 • 158 citations

Human immunodeficiency virus type 1 Nef associates with a member of the p21-activated kinase family

1996 • 154 citations

Association of tyrosine kinase p56lck with CD4 inhibits the induction of growth through the αβ T-cell receptor

1992 • 146 citations

A Conserved Domain and Membrane Targeting of Nef from HIV and SIV Are Required for Association with a Cellular Serine Kinase Activity

1995 • 122 citations

Nef from primary isolates of human immunodeficiency virus type 1 suppresses surface CD4 expression in human and mouse T cells

1993 • 118 citations

Human immunodeficiency virus type 1 Nef and p56lck protein-tyrosine kinase interact with a common element in CD4 cytoplasmic tail.

1995 • 100 citations

The p60c-src family of protein-tyrosine kinases: structure, regulation, and function.

1992 • 97 citations

The HCF repeat is an unusual proteolytic cleavage signal.

1995 • 94 citations

The cytoplasmic domain of CD4 is sufficient for its down-regulation from the cell surface by human immunodeficiency virus type 1 Nef

1994 • 89 citations

CD4 cell surface downregulation in HIV-1 Nef transgenic mice is a consequence of intracellular sequestration.

1993 • 87 citations

Severe immunodeficiency associated with a human immunodeficiency virus 1 NEF/3'-long terminal repeat transgene.

1994 • 84 citations

Mutational Analysis of HIV-1 Nef: Identification of Two Mutants That Are Temperature-Sensitive for CD4 Downregulation

1996 • 76 citations

Specific Th1 cytokine down-regulation associated with primary clinically derived human immunodeficiency virus type 1 Nef gene-induced expression.

1996 • 76 citations

The HIV-1 Nef Protein Interferes with Phosphatidylinositol 3-Kinase Activation 1

1996 • 56 citations

The association of Nef with a cellular serine/threonine kinase and its enhancement of infectivity are viral isolate dependent

1996 • 56 citations

HIV-1 Nef inhibits a common activation pathway in NIH-3T3 cells.

1994 • 54 citations

Towards unraveling the complexity of T cell signal transduction

1995 • 33 citations

Transient assay for Nef-induced down-regulation of CD4 antigen expression on the cell surface

1995 • 8 citations