Export of Protein: A Biochemical View

Patterns of Amino Acids near Signal‐Sequence Cleavage Sites

1983 • 2,290 citations

The spontaneous insertion of proteins into and across membranes: The helical hairpin hypothesis

1981 • 868 citations

Binding of a specific ligand inhibits import of a purified precursor protein into mitochondria

1986 • 723 citations

Secretory protein translocation across membranes—the role of the ‘docking protein’

1982 • 648 citations

Translocation of proteins across the endoplasmic reticulum. I. Signal recognition protein (SRP) binds to in-vitro-assembled polysomes synthesizing secretory protein.

1981 • 644 citations

Protein translocation across the endoplasmic reticulum. II. Isolation and characterization of the signal recognition particle receptor.

1982 • 478 citations

Transport of proteins into mitochondria: Translocational intermediates spanning contact sites between outer and inner membranes

1985 • 470 citations

Protein localization in E. coli: Is there a common step in the secretion of periplasmic and outer-membrane proteins?

1981 • 359 citations

In vitro protein translocation across the yeast endoplasmic reticulum: ATP-dependent post-translational translocation of the prepro-α-factor

1986 • 332 citations

Purification of microsomal signal peptidase as a complex.

1986 • 313 citations

Sequence of the leader peptidase gene of Escherichia coli and the orientation of leader peptidase in the bacterial envelope.

1983 • 307 citations

Proteins of rough microsomal membranes related to ribosome binding. I. Identification of ribophorins I and II, membrane proteins characteristics of rough microsomes

1978 • 281 citations

Evidence for specificity at an early step in protein export in Escherichia coli

1985 • 272 citations

Mutations which alter the function of the signal sequence of the maltose binding protein of Escherichia coli

1980 • 268 citations

Accumulation of glyceride-containing precursor of the outer membrane lipoprotein in the cytoplasmic membrane of Escherichia coli treated with globomycin.

1980 • 266 citations

Post-translational cleavage of presecretory proteins with an extract of rough microsomes from dog pancreas containing signal peptidase activity.

1977 • 259 citations

Effects of two sec genes on protein assembly into the plasma membrane of Escherichia coli.

1985 • 217 citations

A defined mutation in the protein export gene within the spc ribosomal protein operon of Escherichia coli: isolation and characterization of a new temperature-sensitive secY mutant.

1984 • 216 citations

Secretion in yeast: Reconstitution of the translocation and glycosylation of α-factor and invertase in a homologous cell-free system

1986 • 216 citations

Different exported proteins in E. coli show differences in the temporal mode of processing in vivo

1981 • 203 citations

The cleavable prepiece of an imported mitochondrial protein is sufficient to direct cytosolic dihydrofolate reductase into the mitochondrial matrix

1984 • 199 citations

EVALUATION OF COLLOIDAL GOLD AS A CYTOCHEMICAL MARKER FOR TRANSMISSION AND SCANNING ELECTRON MICROSCOPY

1979 • 196 citations

Outer Membrane of Salmonella

1973 • 184 citations

secD, a new gene involved in protein export in Escherichia coli

1987 • 173 citations

Role for membrane potential in the secretion of protein into the periplasm of Escherichia coli.

1981 • 173 citations

Energy is Required for Maturation of Exported Proteins in Escherichia coli

1981 • 157 citations

Characterization of new membrane lipoproteins and their precursors of Escherichia coli.

1981 • 146 citations

Proteins of rough microsomal membranes related to ribosome binding. II. Cross-linking of bound ribosomes to specific membrane proteins exposed at the binding sites

1978 • 142 citations

Secretion of beta-lactamase into the periplasm of Escherichia coli: evidence for a distinct release step associated with a conformational change.

1986 • 133 citations

Mechanism of signal peptide cleavage in the biosynthesis of the major lipoprotein of the Escherichia coli outer membrane.

1982 • 133 citations

The requirement for energy during export of beta-lactamase in Escherichia coli is fulfilled by the total protonmotive force.

1984 • 132 citations

An Escherichia coli mutant with an amino acid alteration within the signal sequence of outer membrane prolipoprotein.

1978 • 131 citations

In Vivo Function and Membrane Binding Properties Are Correlated for Escherichia coli lamB Signal Peptides

1985 • 130 citations

The SecY membrane component of the bacterial protein export machinery: analysis by new electrophoretic methods for integral membrane proteins.

1985 • 129 citations

Localization and processing of outer membrane and periplasmic proteins in Escherichia coli strains harboring export-specific suppressor mutations.

1982 • 124 citations

Nucleotide sequence of the Escherichia coli prolipoprotein signal peptidase (lsp) gene.

1984 • 116 citations

Mechanisms of membrane assembly: effects of energy poisons on the conversion of soluble M13 coliphage procoat to membrane-bound coat protein.

1980 • 115 citations

Energetics and intermediates of the assembly of Protein OmpA into the outer membrane of Escherichia coli.

1983 • 114 citations

The nature of information, required for export and sorting, present within the outer membrane protein OmpA of Escherichia coli K-12.

1985 • 114 citations

Biosynthesis of Mitochondrial Porin and Insertion into the Outer Mitochondrial Membrane of Neuruspora crassa

1982 • 113 citations

Selectivity of Intracellular Proteolysis: Protein Substrates Activate the ATP-Dependent Protease (La)

1986 • 112 citations

Filamentous phage pre-coat is an integral membrane protein: Analysis by a new method of membrane preparation

1982 • 112 citations

Intragenic suppressor mutations that restore export of maltose binding protein with a truncated signal peptide

1984 • 111 citations

Import of proteins into mitochondria. In vitro studies on the biogenesis of the outer membrane.

1983 • 102 citations

Protein export in Escherichia coli requires a soluble activity.

1984 • 100 citations

Intergration of the receptor for bacteriophage lambda in the outer membrane of Escherichia coli: coupling with cell division

1975 • 99 citations

Role of the mature protein sequence of maltose-binding protein in its secretion across the E. coli cytoplasmic membrane

1981 • 69 citations

Molecular cloning and sequencing of the sppA gene and characterization of the encoded protease IV, a signal peptide peptidase, of Escherichia coli.

1986 • 66 citations

Post-translational export of maltose-binding protein in Escherichia coli strains harboring malE signal sequence mutations and either prl+ or prl suppressor alleles.

1985 • 63 citations

Protease IV, a cytoplasmic membrane protein of Escherichia coli, has signal peptide peptidase activity.

1984 • 63 citations

Translocation of nascent secretory proteins across membranes can occur late in translation.

1986 • 62 citations

Requirement for Signal Peptide Cleavage of Escherichia coli Prolipoprotein

1983 • 57 citations

ULTRASTRUCTURE AND ORGANIZATION OF THE BACTERIAL ENVELOPE*

1974 • 52 citations

Normal precursors of periplasmic proteins accumulated in the cytoplasm are not exported post‐translationally in Escherichia coli

1984 • 50 citations

Chapter 3 The Use of Genetic Techniques to Analyze Protein Export in Escherichia coli

1985 • 50 citations

The use of extragenic suppressors to define genes involved in protein export in Escherichia coli

1984 • 49 citations

Suppressors of the secY24 mutation: identification and characterization of additional ssy genes in Escherichia coli

1986 • 49 citations

Mapping of the lipoprotein signal peptidase gene (lsp)

1984 • 30 citations

A signal sequence mutant defective in export of ribose-binding protein and a corresponding pseudorevertant isolated without imposed selection.

1985 • 28 citations

Procoat, the precursor of M13 coat protein, inserts post-translationally into the membrane of cells infected by wild-type virus

1981 • 23 citations

Secretion and processing of ribose-binding protein in Escherichia coli.

1982 • 23 citations

Functional implications of secondary structure analysis of wild type and mutant bacterial signal peptides.

1981 • 17 citations

Secretory S complex of Bacillus subtilis forms a large, organized structure when released from ribosomes.

1985 • 15 citations