Genetic Studies on Protein Export in Bacteria

TnphoA: a transposon probe for protein export signals.

1985 • 1,030 citations

Signal recognition particle contains a 7S RNA essential for protein translocation across the endoplasmic reticulum

1982 • 766 citations

E. coli mutant pleiotropically defective in the export of secreted proteins

1981 • 512 citations

Purification of a membrane-associated protein complex required for protein translocation across the endoplasmic reticulum.

1980 • 502 citations

GENETIC ANALYSIS OF PROTEIN EXPORT IN ESCHERICHIA COLI K12

1985 • 432 citations

Suppressor mutations that restore export of a protein with a defective signal sequence

1981 • 382 citations

Protein localization in E. coli: Is there a common step in the secretion of periplasmic and outer-membrane proteins?

1981 • 359 citations

Regulation of a membrane component required for protein secretion in escherichia coli

1982 • 356 citations

Mechanism of Incorporation of Cell Envelope Proteins in Escherichia Coli

1982 • 344 citations

Secretion cloning vectors in Escherichia coli.

1984 • 322 citations

Mutations that alter the signal sequence of alkaline phosphatase in Escherichia coli

1983 • 281 citations

Evidence for specificity at an early step in protein export in Escherichia coli

1985 • 272 citations

Mutations in a new gene, secB, cause defective protein localization in Escherichia coli

1983 • 270 citations

Use of gene fusion to study secretion of maltose-binding protein into Escherichia coli periplasm

1979 • 267 citations

Uses of lac fusions for the study of biological problems

1985 • 261 citations

Thespcrtbosomal protein operon ofEschenchia coli:sequence and cotranscriptlon of the rlbosomal protein genes and a protein export gene

1983 • 258 citations

Disassembly and reconstitution of signal recognition particle

1983 • 242 citations

Fusions of secreted proteins to alkaline phosphatase: an approach for studying protein secretion.

1985 • 238 citations

Analysis of the distribution of charged residues in the N-terminal region of signal sequences: implications for protein export in prokaryotic and eukaryotic cells.

1984 • 230 citations

A defined mutation in the protein export gene within the spc ribosomal protein operon of Escherichia coli: isolation and characterization of a new temperature-sensitive secY mutant.

1984 • 216 citations

A temperature-sensitive mutant of E. coli exhibiting slow processing of exported proteins

1983 • 215 citations

Conditional-lethal mutations that suppress genetic defects in morphogenesis by altering structural proteins.

1975 • 212 citations

Eukaryotic signal sequence transports insulin antigen in Escherichia coli.

1980 • 203 citations

Evidence for posttranslational translocation of β-lactamase across the bacterial inner membrane

1982 • 153 citations

The ultimate localization of an outer membrane protein of Escherichia coli K-12 is not determined by the signal sequence.

1983 • 151 citations

Secretion of Interferon by Bacillus subtilis

1983 • 150 citations

Determinants for protein localization: beta-lactamase signal sequence directs globin across microsomal membranes.

1984 • 149 citations

A signal sequence is not sufficient to lead β-galactosidase out of the cytoplasm

1980 • 149 citations

Use of gene fusions to study outer membrane protein localization in Escherichia coli.

1977 • 148 citations

Bacteria mature preproinsulin to proinsulin.

1980 • 143 citations

The nucleotide sequence of the gene for malF protein, an inner membrane component of the maltose transport system of Escherichia coli. Repeated DNA sequences are found in the malE-malF intercistronic region.

1984 • 141 citations

Identification of a new gene (secA) and gene product involved in the secretion of envelope proteins in Escherichia coli

1982 • 134 citations

Secretion of Escherichia coli beta-lactamase from Bacillus subtilis by the aid of alpha-amylase signal sequence.

1982 • 131 citations

Uses of lac fusions for the study of biological problems.

1985 • 129 citations

Escherichia coli mutants accumulating the precursor of a secreted protein in the cytoplasm

1979 • 129 citations

The role of the beta-lactamase signal sequence in the secretion of proteins by Escherichia coli.

1984 • 128 citations

The ompA signal peptide directed secretion of Staphylococcal nuclease A by Escherichia coli.

1985 • 128 citations

A previously unidentified gene in the spc operon of Escherichia coli K12 specifies a component of the protein export machinery

1982 • 126 citations

Localization and processing of outer membrane and periplasmic proteins in Escherichia coli strains harboring export-specific suppressor mutations.

1982 • 124 citations

Diverse effects of mutations in the signal sequence on the secretion of β-lactamase in Salmonella typhimurium

1982 • 121 citations

Identification of the secY (prlA) gene product involved in protein export in Escherichia coli

1984 • 119 citations

Failure of E. coli K-12 to transport PhoE-LacZ hybrid proteins out of the cytoplasm.

1985 • 117 citations

Mutations altering the cellular localization of the phage lambda receptor, an Escherichia coli outer membrane protein.

1978 • 106 citations

Periplasmic enzymes in gram-negative bacteria

1979 • 101 citations

Protein export in Escherichia coli requires a soluble activity.

1984 • 100 citations

Conversion of beta-galactosidase to a membrane-bound state by gene fusion.

1976 • 100 citations

Alkaline phosphatase and OmpA protein can be translocated posttranslationally into membrane vesicles of Escherichia coli

1985 • 98 citations

A prokaryotic membrane anchor sequence: carboxyl terminus of bacteriophage f1 gene III protein retains it in the membrane.

1982 • 94 citations

Segregation of mutant ovalbumins and ovalbumin-globin fusion proteins in Xenopus oocytes

1984 • 89 citations

Transcription and translation of foreign genes in Bacillus subtilis by the aid of a secretion vector

1985 • 87 citations

Nine amino acid residues at the NH2-terminal of lipoprotein are sufficient for its modification, processing, and localization in the outer membrane of Escherichia coli.

1984 • 87 citations

Biosynthesis and periplasmic segregation of human proinsulin in Escherichia coli.

1981 • 81 citations

Sequence information within the lamB gene is required for proper routing of the bacteriophage λ receptor protein to the outer membrane of Escherichia coli K-12

1982 • 79 citations

Bacillus licheniformis penicillinase synthesized in Escherichia coli contains covalently linked fatty acid and glyceride.

1981 • 76 citations

Proper interaction between at least two components is required for efficient export of proteins to the Escherichia coli cell envelope

1985 • 74 citations

Trans‐membrane Translocation of Proteins

1980 • 71 citations

Intragenic regions required for LamB export.

1984 • 70 citations

An ‘internal” signal sequence directs secretion and processing of proinsulin in bacteria

1981 • 68 citations

Molecular components of the signal sequence that function in the initiation of protein export.

1982 • 61 citations

Mutation that suppresses the protein export defect of the secY mutation and causes cold-sensitive growth of Escherichia coli

1984 • 54 citations

The product of gene secC is involved in the synthesis of exported proteins in E. coli

1984 • 52 citations

In vitro construction and characterization of phoA-lacZ gene fusions in Escherichia coli

1983 • 50 citations

Identification of five new essential genes involved in the synthesis of a secreted protein in Escherichia coli

1985 • 49 citations

The use of extragenic suppressors to define genes involved in protein export in Escherichia coli

1984 • 49 citations

Signal sequence mutations disrupt feedback between secretion of an exported protein and its synthesis in E. coli

1984 • 46 citations

Escherichia coli 6S RNA is not essential for growth or protein secretion

1985 • 45 citations

Synthesis and secretion of biologically active mouse Interferon-β using a Bacillus subtilis α-amylase secretion vector

1985 • 41 citations

Efficient expression of cloned complementary DNAs for secretory proteins after injection into Xenopus oocytes

1984 • 41 citations

Pseudomonas Aeruginosa Secretes and Correctly Processes Human Growth Hormone

1984 • 41 citations

Export and processing of MalE-LacZ hybrid proteins in Escherichia coli

1984 • 35 citations

Gene encoding a hybrid OmpF-PhoE pore protein in the outer membrane of Escherichia coli K12

1984 • 35 citations

Mutation prlF1 relieves the lethality associated with export of beta-galactosidase hybrid proteins in Escherichia coli

1984 • 33 citations

Genetics of bacteria

1985 • 28 citations

Effects of secA mutations on the synthesis and secretion of proteins in Escherichia coli. Evidence for a major export system for cell envelope proteins.

1986 • 27 citations

Secretion and processing of an immunoglobulin light chain in Escherichia coli

1984 • 26 citations

Characterization of an amber mutation in the structural gene for ribosomal protein L15, which impairs the expression of the protein export gene, secY, in Escherichia coli.

1984 • 25 citations

Biosynthesis of Bacillus licheniformis penicillinase in Escherichia coli and in Bacillus subtilis

1983 • 20 citations

Expression of chemically synthesized -neo-endorphin gene fused to E. coli alkaline phosphatase

1983 • 19 citations

Alkaline Phosphatase Fusions: A Tag to Identify Mutations that Result in Increased Expression of Secreted Human Growth Hormone from Escherichia coli

1986 • 19 citations

Both linked and unlinked mutations can alter the intracellular site of synthesis of exported proteins of Escherichia coli

1985 • 16 citations

The penicillinase of Bacillus licheniformis is an outer membrane protein in Escherichia coli

1983 • 14 citations

Genetic control of protein secretion and localization

1986 • 11 citations

Selection for mutants altered in the expression or export of outer membrane porin OmpF

1985 • 10 citations

Use of Gene Fusions to Study Biological Problems

1984 • 6 citations